Fig 1: Changes in KIT gene expression by GATA1 siRNAs. (a) qRT-PCR analysis was performed for K562-WT (left) and one of the K562-G1s clone, C1 #26 (right). The vertical axis represents a relative value with each value of non-target siRNA set at 1. Results are averages of three independent experiments. Error bars represent standard deviation. *P < 0.05, **P < 0.01 (two-side t-test). (b) Representative immunoblots of siRNA experiments with K562-WT (left) and G1s C1 #26 (right). Western blotting was performed with anti-GATA1 antibody (D24E4, Cell signaling technology), anti-GATA2 (AF2046, R&D) anti-c-Kit (SC-17806, Santa Cruz) and anti-ß-actin antibody (Sigma-Aldrich). Images were obtained using ChemiDoc MP.