Fig 2: Inhibition of proliferation of T cells stimulated with OKT3/IL2 in the presence or absence of HLA-G, and with or without HLA-G- or LILRB1-blocking antibodies. A: Scheme of the interaction between 4H84 coated beads with HLA-G5. B: Cell surface expression of LILRB1 on lymphocytes analyzed by FASC using the CD85J mAb. The isotype control mAb was tested on resting T cells (Iso). LILRB1 is expressed both on resting and activated T cells. C: Thymidine incorporation into T cells stimulated with OKT3/IL2 and incubated or not with HLA-G- or LILRB1-blocking antibodies or the HLA-G-blocking antibody (87G) D: The inhibition of T-cell proliferation associated with HLA-G5 was dramatically reduced when the HLA-G-inhibitory mAb(87G)- or LILRB1-blocking antibody were added to T cells in the presence of HLA-G.

Fig 3: Immuno-precipitation of ILT-2, SHP-1, or SHP-2 in T cells in the presence or absence of HLA-G.T cells were stimulated with OKT3/IL2, and were incubated with HLA-G5-coated beads for 0, 15 or 30 min. After cell lysis, LILRB1, SHP-1, or SHP-2 were immuno-purified and the co-precipitated molecules were submitted to gel electrophoresis and then transferred to a PVDF membrane. SHP-1, SHP-2, and LILRB1 were detected by western-blot analysis. A: Immunoprecipitation of LILRB1. LILRB1, SHP-1 and SHP-2 were detected by western blot. The ratio of SHP-1 or SHP-2/LILRB1 have been determined by densitometry. B: Immunoprecipitation of SHP-1. LILRB1 and SHP-1 were detected by western blot. The ratio of LILRB1/SHP-1 has been determined by densitometry. C Immunoprecipitation of SHP-2. LILRB1 and SHP-2 were detected by western blot. The ratio of LILRB1/SHP-2 has been determined by densitometry.