Fig 2: Effect of PHC on Fas protein expression in the lung alveolar area of rats. In the T/HS, PHC1 and PHC2 groups, the protein levels of Fas were markedly increased compared with the sham group. However, Fas protein expression was decreased in the PHC1 and PHC2 groups compared with the T/HS group. Black arrows indicate Fas protein expression. PHC, penehyclidine hydrochloride; T/HS, blunt chest trauma and hemorrhagic shock.

Fig 3: Effect of PHC on the expression of Fas, FasL, caspase-3 and caspase-8. (A) Western blotting was performed to assess protein levels of Fas, FasL, caspase-3 and caspase-8. (B) Statistical analysis of (B) Fas and FasL, and (C) caspase-3 and caspase-8. Increased levels of all proteins were observed in the lung tissue of T/HS, PHC1 and PHC2 treated rats when compared with the Sham group. However, protein levels were decreased in the PHC1 and PHC2 groups compared with the T/HS group. Data are expressed as mean ± standard error of the mean. *P<0.05 vs. the Sham group; #P<0.05 vs. the T/HS group. PHC, penehyclidine hydrochloride; FasL, Fas ligand; T/HS, blunt chest trauma and hemorrhagic shock.