Fig 1: GCN5 recruitment to gluconeogenic gene promoters is regulated by cAMP in a CITED2- and PGC-1a-dependent manner in primary hepatocytes.(a) ChIP-qPCR analysis of the occupancy of G6pc and Pck1 promoters with GCN5 in cells depleted of CITED2 and exposed to pCPT-cAMP for the indicated times. (b) ChIP-qPCR analysis of the occupancy of the G6pc promoter with CBP or epigenomic marks in cells depleted of GCN5 or CITED2 and exposed to pCPT-cAMP for the indicated times. (c) ChIP-qPCR analysis of the occupancy of the G6pc promoter with GCN5, CBP or epigenomic modifications in cells depleted of PGC-1a and exposed to pCPT-cAMP for 6 h. (d) ChIP-qPCR analysis of the occupancy of the G6pc promoter with FLAG-tagged PGC-1a, HNF-4a or FLAG-FoxO1 in cells depleted of GCN5, CITED2 or PGC-1a and exposed to pCPT-cAMP for 6 h. All data are means±s.e.m. (n=3). *P<0.05, **P<0.01 versus control or as indicated (ANOVA with Bonferroni's post hoc test). Adenoviral vectors encoding GCN5, CITED2 or PGC-1a shRNAs, FLAG–PGC-1a or FLAG-FoxO1 were used for these experiments. ANOVA, analysis of variance; qPCR, quantitative PCR.