Immunohistochemistry (Paraffin) analysis of RAP1 in human testis tissue sections using the RAP1 Monoclonal Antibody (Product # MA5-37708) at a dilution of 1:25. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1:25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.