Human LIN-28A Antibody AF3757 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemHuman LIN-28A Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $519.00Supplier Page View Company Product Page
Catalog NumberAF3757
NCBI Gene Aliaseslin-28A, CSDD1, LIN28, LIN-28, ZCCHC1
ApplicationsImmunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Simple Western,Immunocytochemistry
ReactivityHuman,Mouse,Primate - Macaca mulatta (Rhesus Macaque),Primate - Pan troglodytes (Chimpanzee),Transgenic Mouse
TargetLIN-28A
Quantity100 ug (also 25 ug)
Conjugate/TagUnconjugated
IsotypeIgG
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
HostGoat
NCBI Full Gene Namelin-28 homolog A
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant human LIN-28A, Met1-Asn209, Accession # Q9H9Z2
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Unconjugated

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Proteomic analysis of cisplatin-induced spermatogenesis defects in miceAnnals of MedicineDecember 31, 2026Pengyu Li, Ziran Chen, Zexuan Zhang, Hongbin Li, Yingnan Zhangtemperature to reduce nonspecific binding. Primary antibodies used were: anti-LIN28A (R&D Systems, AF3757) and anti-ZBTB16 (Santa Cruz, sc-28319) at a 1:100 dilution, anti-SYCP3 (ab97672; Abcam) and anti-CDK1

(2) RUNX proteins and CBFβ create an interconnected network of transcriptional regulation in the male germline†Biology of ReproductionApril 13, 2026Mustika Rahmawati, Carson J Black, Danika D Dalvi, Amanda J Brown, Kassie M Stadler, Tia M Hoisington, Nathan C LawFlow cytometry, CUT&RUN, IP, WB,","IF, Flow: 1:200CUT&RUN: 1 μgIP: 2 μgWB 1:500" Goat anti-LIN28A,"R&D Systems, AF3757","IF, Flow cytometry",IF 1:250Flow cytometry 1:125 Goat anti-cKIT,"R&D Systems, AF1356",IF,1:125 Goat

(3) LIN28A-Dependent Kinome and Phosphoproteome Reprogramming Promotes Imatinib ResistanceMolecular & Cellular ProteomicsJanuary 19, 2026Owen F.J. Hovey, Mallory I. Frederick, Quan M. Quach, Jenica H. Kakadia, Alyssa Wu, Kyle Yang, Tingting Wu, Xiang Ruan, Tomonori Kaneko, Courtney Voss, et al.min), membranes were incubated overnight at 4 °C with primary antibodies against: LIN28A (Bio-Techne, AF3757), GAPDH (CST, #97166), PTEN (CST #13866), RICTOR (Bethyl Laboratories, A300-459A) GSK3-α/β (Santa Cruz,

(4) ZBTB16/PLZF regulates juvenile spermatogonial stem cell development through an extensive transcription factor poising networkNature Structural & Molecular BiologyMarch 3, 2025Chongil Yi, Yuka Kitamura, So Maezawa, Satoshi H. Namekawa, Bradley R. Cairns1:100 (10.0 µg ml−1)), rabbit anti-pH2AX (Cell Signaling, 9718; 1:400), goat anti-LIN28A (R&D systems, AF3757; 1:400), rabbit anti-SALL4 (Abcam, ab29112; 1:1,000 (1 µg ml−1)), mouse anti-SOX3 (Santa Cruz, sc-101155;

(5) Claudin-11 regulates immunological barrier formation and spermatogonial proliferation through stem cell factorCommun BiolJanuary 30, 2025Taichi Sugawara, Kayoko Sonoda, Nattapran Chompusri, Kazuhiro Noguchi, Seiji Okada, Mikio Furuse, Tomohiko WakayamaSigma–Aldrich); goat polyclonal anti-PLZF (1:300; #AF2944; R&D Systems); goat polyclonal anti-LIN28A (1:300; #AF3757; R&D Systems); goat polyclonal anti-KIT (1:300; #AF1356; R&D Systems); rabbit polyclonal anti-SCP3 (1:300;

(6) ARF6, a component of intercellular bridges, is essential for spermatogenesis in miceDevelopmental biologyApril 1, 2024Wong HN, Chen T, Wang PJ, Holzman LB.et al., 2006), anti-ARF6 (rabbit, Abcam ab131261, used at 1:250) and anti-LIN28 (goat, R&D Systems AF3757, used at 1:250) antisera, Alexa Fluor −488, −594 and −647 conjugated secondary antibodies (Invitrogen

(7) Transgene-Free Cynomolgus Monkey iPSCs Generated under Chemically Defined ConditionsCellsMarch 21, 2024Tereshchenko Y, Esiyok N, Garea-Rodríguez E, Repetto D, Behr R, Rodríguez-Polo I.(cTNT),Miltenyi Biotec,130-106-687,1:10 Primary Antibodies,LIN28,"R&D systems (Minneapolis, MN, USA)",AF3757,1:300 Primary Antibodies,NANOG,"Cell Signalling (Danvers, MA, USA)",4903,1:400 Primary Antibodies,OCT4A,Cell

(8) Core binding factor subunit β plays diverse and essential roles in the male germlineFrontiers in Cell and Developmental BiologyNovember 2, 2023Mustika Rahmawati, Kassie M. Stadler, Blanca Lopez-Biladeau, Tia M. Hoisington, Nathan C. Law#,Application,Concentration Rabbit anti CBFβ,"Novus Biologicals, NBP1-87300",IF,1:200 Goat anti LIN28A,"R&D Systems, AF3757","IF, NanoString",IF 1:250 NanoString 1:125 Goat anti cKIT,"R&D Systems, AF1356","IF, IHC",1:125 Rabbit

(9) Inactivation of Tumor Suppressor CYLD Inhibits Fibroblast Reprogramming to PluripotencyCancersOctober 15, 2023Bekas N, Samiotaki M, Papathanasiou M, Mokos P, Pseftogas A, Xanthopoulos K, Thanos D, Mosialos G, Dafou D.Santa Cruz Biotechnology, Dallas, TX, USA), NANOG (C-4, sc-376915, Santa Cruz Biotechnology, Dallas, TX, USA) and LIN28 (AF3757, R&D Systems, Minneapolis, MN, USA). As a secondary antibody, Alexa Fluor™ 555 Highly Cross-Adsorbed

(10) Semi-automated optimized method to isolate CRISPR/Cas9 edited human pluripotent stem cell clonesStem cell research & therapyApril 27, 2023Frank E, Cailleret M, Nelep C, Fragner P, Polentes J, Herardot E, El Kassar L, Giraud-Triboult K, Monville C, Ben M'Barek K.tritonX100 and then incubated overnight with OCT3/4 (Santa Cruz, sc-5279, 1:100) and LIN28A (R&D SYSTEMS, AF3757, 1:200). The day after, cells were washed and incubated with corresponding secondary antibodies (Invitrogen).

(11) Permissive epigenomes endow reprogramming competence to transcriptional regulatorsNature chemical biologyJanuary 1, 2021Kim KP, Choi J, Yoon J, Bruder JM, Shin B, Kim J, Arauzo-Bravo MJ, Han D, Wu G, Han DW, et al.

(12) Non-Human Primate iPSC Generation, Cultivation, and Cardiac Differentiation under Chemically Defined ConditionsCellsMay 29, 2020Michael Stauske et al.,Miltenyi Biotec,130-106-687,1:10 Cardiac troponin T (cTNT) 2,Thermo Fisher,MS295PABX,1:200 LIN28,R&D Systems,AF3757,1:300 Myosin light chain 2a (MLC2a),Synaptic Systems,311-011,1:200 NANOG,Cell Signalling,4903,1:400 SALL4,Abcam,ab57577,1:200 Smooth muscle actin

(13) Modeling of early neural development in vitro by direct neurosphere formation culture of chimpanzee induced pluripotent stem cellsStem cell researchApril 1, 2020Kitajima R, Nakai R, Imamura T, Kameda T, Kozuka D, Hirai H, Ito H, Imai H, Imamura M.

(14) Improvement of transfection with reprogramming factors in urinederived cellsBIOCELLJanuary 1, 2020OLIVIA A. ROBLES-RODR虶UEZ, MAR虯 J. LOERA-ARIAS, JOS�J. P蒖EZ-TRUJILLO, ARNULFO VILLANUEVA-OLIVO, ERNESTO PIC覰-GALINDO, LAURA VILLARREAL-MARTNEZ, ADOLFO SOTO-DOM蚇GUEZ, HUMBERTO RODR虶UEZROCHA ARACELY GARC虯-GARC虯, ODILA SAUCEDO-C罵DENAS, ROBERTO MONTES DE OCA-LUNAR & D Systems, Minneapolis, MN, USA, #AF1997), anti-Lin28 (1:50; R&D Systems, Minneapolis, MN, USA, #AF3757), anti-Oct4 (1:100; R&D Systems, Minneapolis, MN, USA, #MAB17591), and anti-Klf4 (1:50; R & D Systems,

(15) Exonuclease Domain-Containing 1 Enhances MIWI2 piRNA Biogenesis via Its Interaction with TDRD12Cell reportsSeptember 25, 2018Pandey RR, Homolka D, Olotu O, Sachidanandam R, Kotaja N, Pillai RS.

(16) Derivation of induced pluripotent stem cells in Japanese macaque (Macaca fuscata)Scientific reportsAugust 15, 2018Nakai R, Ohnuki M, Kuroki K, Ito H, Hirai H, Kitajima R, Fujimoto T, Nakagawa M, Enard W, Imamura M.(BD Biosciences, 611202), rabbit anti-NANOG (REPROCELL, RCAB0003P), goat anti-LIN28A (R&D systems, AF3757), rabbit anti-DPPA4 (Abcam, ab154642), mouse anti-SOX2 (R&D systems, MAB2018), rabbit anti-SALL4 (Abcam,

(17) Biallelic loss of human CTNNA2, encoding αN-catenin, leads to ARP2/3 complex overactivity and disordered cortical neuronal migrationNature geneticsAugust 1, 2018Schaffer AE, Breuss MW, Caglayan AO, Al-Sanaa N, Al-Abdulwahed HY, Kaymakçalan H, Yılmaz C, Zaki MS, Rosti RO, Copeland B, et al.

(18) Identification of dynamic undifferentiated cell states within the male germlineNature communicationsJuly 19, 2018La HM, Mäkelä JA, Chan AL, Rossello FJ, Nefzger CM, Legrand JMD, De Seram M, Polo JM, Hobbs RM.anti-c-KIT (AF1356, 1:250), anti-mouse/rat PDX1 (AF2517, 1:250), anti-PLZF (AF2944, 1:500), anti-LIN28A (AF3757, 1:500) and anti-SOX3 (AF2569, 1:250) (R&D Systems), rabbit anti-mCherry (ab167453, 1:2000), anti-OCT4

(19) Autologous and Heterologous Cell Therapy for Hemophilia B toward Functional Restoration of Factor IXCell reportsMay 1, 2018Ramaswamy S, Tonnu N, Menon T, Lewis BM, Green KT, Wampler D, Monahan PE, Verma IM.

(20) Modeling Short QT Syndrome Using Human-Induced Pluripotent Stem Cell-Derived CardiomyocytesJournal of the American Heart AssociationMarch 24, 2018El-Battrawy I, Lan H, Cyganek L, Zhao Z, Li X, Buljubasic F, Lang S, Yücel G, Sattler K, Zimmermann WH, et al.rabbit IgG 1:100 Thermo Fisher Scientific, #PA1-097 hLIN28 polyclonal goat IgG 1:300 R&D Systems, #AF3757 hSSEA4 monoclonal mouse IgG3 1:100 Thermo Fisher Scientific, #MA1-021 hTRA-1-60 monoclonal mouse

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Human LIN-28A Antibody

LIN-28A Antibody

LIN-28A Antibody

LIN-28A Antibody

LIN-28A Antibody

Human LIN-28A Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (34)

Reviews

Human LIN-28A Antibody from R&D Systems, a Bio-Techne Brand

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