Fig 2: The expression and location of ANKHD1 in clinical samples and UCEC cells.Immunohistochemistry of ANKHD1 (a) between metastasis and primary UCEC samples. RT-PCR (b) and Western blot (c) for the expression of ANKHD1 in carcinoma and para-carcinoma. Immunofluorescence staining of ANKHD1 (d). RT-PCR (e) and Western blot (f) of blank, shRNA, and NC group. The result of DNA electrophoresis (g). ****p < 0.0001. Data shown are the mean ± SD of at least three independent experiments.

Fig 3: ANKHD1-BP3 regulates the activity of the PI3K-AKT pathway and the plot of the hypothesis.Bubble maps (a) of the KEGG pathway. ANKHD1-BP3 regulates the activity of the PI3K-AKT pathway in Ishikawa (b). The plot of hypothesis (c) showed that HSPB1 regulated ANKHD1, and the transcript of ANKHD1 was involved in the tyrosine metabolism pathway, which affected the distant metastasis. Figure 8c was created with BioRender.com.