Description
Anti-Rabbit IgG for IP (HRP) is a conformation-specific secondary antibody that binds exclusively to native, non-denatured IgG and does not recognize denatured IgG. Conformation-specific secondary antibodies target spatial epitopes. When antibodies are denatured by high temperatures, they lose their native conformation and are no longer recognized by such antibodies—neither the heavy chain nor the light chain is detected. This effectively avoids interference from the heavy chain (55 kDa) and light chain (25 kDa) in immunoprecipitation (IP) experiments