PAR1/Thrombin Receptor Antibody (N2-11)

Product Specs
ItemPAR1/Thrombin Receptor Antibody (N2-11) - BSA Free
CompanyNovus Biologicals, a Bio-Techne Brand
Price $429.00Supplier Page View Company Product Page
Catalog NumberNBP1-71770
ApplicationsIF/IHC,ELISA,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunofluorescence,Immunocytochemistry/ Immunofluorescence
ReactivityHuman,Mouse
TargetPAR1/Thrombin Receptor
Conjugate/TagUnconjugated
IsotypeIgG1
HostMouse
Quantity0.1 ml (also 0.025 ml)
ImmunogenThe oligopeptide CNATLDPRSFLL from human Thrombin Receptor. [Swiss-Prot# P25116]
Concentration1 mg/ml
FormatPBS
CloneN2-11
NCBI Gene AliasesCF2R, PAR1, Cf2r, TR, HTR, Par1, ThrR, PAR-1
ClonalityMonoclonal
NCBI Full Gene Namecoagulation factor II thrombin receptor, coagulation factor II (thrombin) receptor
PurityProtein G purified
Molecular Weight52 kDa
Research AreaApoptosis, Cancer, GPCR
Antibody TypePrimary Antibodies
StorageStore at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Sizes Available: 0.1 ml (also 0.025 ml)

Questions and Answers

What species does this antibody work with, and has it been used in relevant studies?

The PAR1/Thrombin Receptor Antibody has been shown to react with both human and mouse species. It has been utilized in various studies, including those investigating the role of PAR1 in neural stem cell expansion and differentiation in the subventricular zone of adult mice (), as well as in studies examining its involvement in tumor growth and macrophage interactions in cancer models ().

Can this antibody be used for immunofluorescence, and what has been documented regarding its application?

Yes, the PAR1/Thrombin Receptor Antibody has been successfully used for immunofluorescence applications. For instance, it was employed to assess PAR1 expression in human myometrial cells, demonstrating its role in thrombin-induced contractions (). Additionally, it was used to confirm the presence of PAR1 in neural stem cells within the adult mouse brain ().

Is there any evidence of this antibody being used in studies related to metabolic dysfunction?

Yes, the PAR1/Thrombin Receptor Antibody has been referenced in studies exploring its role in metabolic dysfunction. For example, it was used to investigate the effects of coagulation factors and PAR1 signaling in brown adipose tissue under metabolic stress, highlighting its potential impact on systemic glucose intolerance and brown adipocyte function ().

What applications has this antibody been validated for, based on scientific literature?

The PAR1/Thrombin Receptor Antibody has been validated for use in several applications, including Western blotting, ELISA, immunocytochemistry, immunofluorescence, and immunohistochemistry. Its effectiveness has been documented in studies focusing on neural stem cell behavior, myometrial contractions, and tumor biology, indicating its versatility in various research contexts.

Novus Biologicals, a Bio-Techne Brand

United States: 10771 E Easter Ave

Centennial, Colorado 80112

United States

Phone:
303-730-1950
888-506-6887

Email: novus@novusbio.com

Company Profile

Website: https://www.novusbio.com

(1) Dicer Neosynthesis Regulates Platelet Reactivity: A Mechanism Altered in Type 2 DiabetesCirculation researchAugust 29, 2025Bury L, Piselli E, Manni G, Momi S, Ciarrocca Taranta G, Guglielmini G, Fanelli C, De Fano M, Rowley JW, Weyrich AS, et al.(protease-activated receptor 1) by Western blotting with anti–N-terminus PAR1 antibody (Novus Biologicals; cat.n. NBP1-71770; 1:1000) raised against the soluble amino-terminal thrombin cleavage peptide region of PAR1 as primary

(2) KLK6/PAR1 Axis Promotes Tumor Growth and Metastasis by Regulating Cross-Talk between Tumor Cells and MacrophagesCellsDecember 16, 2022Hwang YS, Cho HJ, Park ES, Lim J, Yoon HR, Kim JT, Yoon SR, Jung H, Choe YK, Kim YH, et al.(Santa Cruz, sc-550609), GFP (Santa Cruz, sc-9996), PAR1 (Novus Biologicals, Novus, Centennial, CO, USA, NBP1-71770), and PAR2 (Abcam, Cambridge, UK, ab180953) antibodies were obtained from Santa Cruz Biotechnology,

(3) Coagulation factors promote brown adipose tissue dysfunction and abnormal systemic metabolism in obesityiScienceJuly 15, 2022Hayashi Y, Shimizu I, Yoshida Y, Ikegami R, Suda M, Katsuumi G, Fujiki S, Ozaki K, Abe M, Sakimura K, et al.size. The following antibodies and fluorescent dyes were used: anti-PAR1 antibody (NOVUS Biologicals, NBP1-71770), anti-Factor VII antibody (Abcam, ab97614), anti-tissue factor antibody (Abcam, ab189483), wheat germ

(4) Intrinsic Expression of Coagulation Factors and Protease Activated Receptor 1 (PAR1) in Photoreceptors and Inner Retinal LayersInternational journal of molecular sciencesJanuary 17, 2022Goldberg Z, Sher I, Qassim L, Chapman J, Rotenstreich Y, Shavit-Stein E.

(5) Mechanisms of thrombin-Induced myometrial contractions: Potential targets of progesteronePloS oneJanuary 1, 2020Nishimura F, Mogami H, Moriuchi K, Chigusa Y, Mandai M, Kondoh E.NBP1-58268, Research Resource Identifier (RRID): AB_11023777, 1:100) and PAR1 (N2-11, Novus Biologicals, NBP1-71770, RRID: AB_11027203, 1:100). Thereafter, sections were incubated with Alexa Fluor 488 (Goat anti-Mouse

(6) Dabigatran Suppresses PAR-1/SphK/S1P Activation of Astrocytes in Experimental Autoimmune Encephalomyelitis ModelFrontiers in molecular neuroscienceJanuary 1, 2020Chen R, Cao X, Luo W, Yang H, Luo X, Yu J, Luo J.

(7) Early initiation of a factor Xa inhibitor can attenuate tissue repair and neurorestoration after middle cerebral artery occlusionBrain researchSeptember 1, 2019Komori M, Ago T, Wakisaka Y, Nakamura K, Tachibana M, Yoshikawa Y, Shibahara T, Yamanaka K, Kuroda J, Kitazono T.

(8) Weisheng-Tang Ameliorates Acute Ischemic Brain Damage in Mice by Maintaining Blood-Brain Barrier IntegrityOxidative medicine and cellular longevityJanuary 1, 2019Kim MJ, Park KH, Lee JY, Ha KT, Choi BT, Baek JU, Yun YJ, Lee SY, Shin HK.

(9) The Thrombin Receptor Restricts Subventricular Zone Neural Stem Cell Expansion and DifferentiationScientific reportsJune 19, 2018Choi CI, Yoon H, Drucker KL, Langley MR, Kleppe L, Scarisbrick IA.and in situ hybridization techniques. PAR1 immunoreactivity was detected with a monoclonal antibody (NBP1-71770, Novus Biologicals, Littleton, CO). PAR1 was co-localized with either Nestin (NB100-1604, Novus Biologicals,

(10) Protease activated receptor-1 antagonist ameliorates the clinical symptoms of experimental autoimmune encephalomyelitis via inhibiting breakdown of blood-brain barrierJournal of neurochemistryNovember 1, 2015Kim HN, Kim YR, Ahn SM, Lee SK, Shin HK, Choi BT.

(11) In vivo nano-imaging of membrane dynamics in metastatic tumor cells using quantum dotsThe Journal of biological chemistryJanuary 22, 2010Gonda K, Watanabe TM, Ohuchi N, Higuchi H.

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