RUNX2/CBFA1 Antibody

Product Specs
ItemRUNX2/CBFA1 Antibody - BSA Free
CompanyNovus Biologicals, a Bio-Techne Brand
Price $429.00Supplier Page View Company Product Page
Catalog NumberNBP1-77461
Conjugate/TagUnconjugated
ImmunogenThis RUNX2/CBFA1 Antibody was prepared from a synthetic peptide made to an internal region of the human RUNX2 protein (within residues 300-375). [Swiss-Prot Q13950]
FormatPBS and 30% Glycerol
ApplicationsIF/IHC,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunocytochemistry/ Immunofluorescence
TargetRUNX2/CBFA1
NCBI Gene AliasesOSF2, CLCD, AML3, CBFA1, PEA2aA, CCD, CBF-alpha-1, PEBP2aA, CCD1, OSF-2
ReactivityHuman,Mouse
Concentration1 mg/ml
HostRabbit
Quantity0.1 ml (also 0.025 ml)
NCBI Full Gene NameRUNX family transcription factor 2
ClonalityPolyclonal
PurityImmunogen affinity purified
Research AreaEpigenetics, Growth and Development, Stem Cells, Transcription Factors and Regulators
IsotypeIgG
Antibody TypePrimary Antibodies
Molecular Weight56.6 kDa
StorageStore at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles

Specifications/Features

Available conjugates: Unconjugated

Questions and Answers

What species does this antibody work with, and has it been used in relevant studies?

The RUNX2/CBFA1 Antibody is reactive with both human and mouse species. It has been utilized in various studies, including those investigating the differentiation of tenocytes into hypertrophic chondrocyte-like cells, where it was used to assess RUNX2 expression in calcified tendon tissues ().

Can this antibody be effectively used in immunohistochemistry, and what evidence supports its use?

Yes, this antibody has been shown to work in immunohistochemistry applications. For instance, it was employed in a study examining cardiac fibroblasts and their osteogenic differentiation, where RUNX2 expression was analyzed in tissue sections to understand its role in pathological heart calcification ().

What specific applications has this antibody been cited in, and how has it contributed to research findings?

The RUNX2/CBFA1 Antibody has been cited in applications such as Western blotting, immunocytochemistry, immunofluorescence, and immunohistochemistry. It has contributed to research by helping to elucidate the role of RUNX2 in processes like nephrocalcinosis and osteogenic differentiation in renal cells, highlighting its importance in understanding mineralization mechanisms (, ).

Is there any information on the concentration or dilution used for this antibody in published studies?

In various studies, the RUNX2/CBFA1 Antibody has been used at dilutions such as 1:200 for immunohistochemistry and 1:50 for in-cell Western assays, demonstrating its versatility and effectiveness in different experimental setups (, ).

Novus Biologicals, a Bio-Techne Brand

United States: 10771 E Easter Ave

Centennial, Colorado 80112

United States

Phone:
303-730-1950
888-506-6887

Email: novus@novusbio.com

Company Profile

Website: https://www.novusbio.com

(1) Identification of novel macrophages and bone morphogenetic protein signals causing ectopic calcification and impairing muscle regenerationiScienceJuly 18, 2025Shi L, He Z, Hiraga T, Liu Z, Yamashita T, Iwamoto R, Mizoguchi T, Nakamichi Y, Kubota Y, Udagawa N, et al.1:200), anti-myogenin rabbit polyclonal (PA-116750, Invitrogen, 1:200), anti-Runx2 rabbit polyclonal (NBP1-77461, Novus Biologicals, 1:200) FITC-conjugated Ki-67 (SolA15) monoclonal (11-5698-82, eBioscience, 1:100)

(2) Exploring the Link Between Autophagy‐Lysosomal Dysfunction and Early Heterotopic Ossification in TendonsAdvanced ScienceJuly 1, 2024Chang‐He Gao, Qian‐Qian Wan, Jan‐Fei Yan, Yi‐Na Zhu, Lei Tian, Jian‐Hua Wei, Bin Feng, Li‐Na Niu, Kai Jiao(Santa Cruz Biotechnology, catalogue number: sc‐376362), LAMP1 (Novus Biologicals, catalogue number: NBP1‐77461), RAB7 (Abcam, catalogue number: Ab137029), and V‐ATP6V1A (Abcam, catalogue number: ab199326). α‐SMA

(3) In vitro development and optimization of cell-laden injectable bioprinted gelatin methacryloyl (GelMA) microgels mineralized on the nanoscaleBiomaterials AdvancesMay 1, 2024Mauricio Gonçalves da Costa Sousa, Gabriela de Souza Balbinot, Ramesh Subbiah, Rahul Madathiparambil Visalakshan, Anthony Tahayeri, Maria Elisa Lima Verde, Avathamsa Athirasala, Genevieve Romanowicz, Robert E. Guldberg, Luiz E. Bertassoniin PBS and incubated with the primary antibody (rabbit polyclonal SOST antibody (Novus Biologicals, NBP1-77461)) (1 : 100 dilution), overnight at 4 °C. After washing with PBS, the samples were incubated with secondary

(4) In vitro development and optimization of cell-laden injectable bioprinted gelatin methacryloyl (GelMA) microgels mineralized on the nanoscalebioRxiv : the preprint server for biologyOctober 12, 2023Sousa MGDC, Balbinot GS, Subbiah R, Visalakshan RM, Tahayeri A, Lima Verde ME, Athirasala A, Romanowicz G, Guldberg RE, Bertassoni LE.

(5) Adseverin, an actin-binding protein, modulates hypertrophic chondrocyte differentiation and osteoarthritis progressionScience advancesAugust 4, 2023Chan B, Glogauer M, Wang Y, Wrana J, Chan K, Beier F, Bali S, Hinz B, Parreno J, Ashraf S, et al.Ihh (1:100; ab39634, Abcam), Ki67 (1:50; ab15580, Abcam), αSMA (1:200; ab5694, Abcam), Runx2 (1:50; NBP1-77461, Novus Biologicals), MMP13 (1:200; 18165-1-AP, Proteintech), and collagen type X (1:1000; ab260040,

(6) Excess Growth Hormone Triggers Inflammation-Associated Arthropathy, Subchondral Bone Loss, and ArthralgiaThe American journal of pathologyJune 1, 2023Poudel SB, Ruff RR, Yildirim G, Dixit M, Michot B, Gibbs JL, Ortiz SD, Kopchick JJ, Kirsch T, Yakar S.

(7) Engineering of an Osteoinductive and Growth Factor-Free Injectable Bone-Like Microgel for Bone RegenerationAdvanced healthcare materialsApril 1, 2023Subbiah R, Lin EY, Athirasala A, Romanowicz GE, Lin ASP, Califano JV, Guldberg RE, Bertassoni LE.staining of RUNX2 and YAP as described above using polyclonal anti-RUNX2 antibody produced in rabbit (NBP1–77461, Novus Biologicals), monoclonal anti-YAP1 produced in mouse (WH0010413M1, Sigma-Aldrich) respectively

(8) A Polymer Canvas with the Stiffness of the Bone Matrix to Study and Control Mesenchymal Stem Cell ResponseAdvanced healthcare materialsApril 1, 2023Zanut A, Li R, Deng R, Liu X, Rejhon M, Chen W, Weck M, de Peppo GM, Riedo E.

(9) Nanoscale mineralization of cell-laden methacrylated gelatin hydrogels using calcium carbonate–calcium citrate core–shell microparticles†Journal of Materials Chemistry BDecember 1, 2021Ramesh Subbiah, Gabriela de Souza Balbinot, Avathamsa Athirasala, Fabricio Mezzomo Collares, Grigoriy Sereda, Luiz E. Bertassoniincubated with the primary antibody (rabbit polyclonal anti-RUNX2/CBFA1 antibody (Novus Biologicals, NBP1–77461)) (1 : 100 dilution), overnight at 4 °C. After washing with PBS, the samples were incubated with secondary

(10) Targeting local lymphatics to ameliorate heterotopic ossification via FGFR3-BMPR1a pathwayNature communicationsJuly 19, 2021Zhang D, Huang J, Sun X, Chen H, Huang S, Yang J, Du X, Tan Q, Luo F, Zhang R, et al.incubated at 4 °C overnight with primary antibodies including Sox9 (1:200; ab185230, Abcam), Runx2 (1:100; NBP1-77461, Novusbio), CD31 (1:100; FAB3628G, R&D Systems), LYVE1 (1:200; ab14917, Abcam/1:100; 14-0443-82, eBioscience),

(11) Involvement of miR-30a-5p and miR-30d in Endothelial to Mesenchymal Transition and Early Osteogenic Commitment under Inflammatory Stress in HUVECBiomoleculesFebruary 5, 2021Ciavarella C, Motta I, Vasuri F, Fittipaldi S, Valente S, Pollutri D, Ricci F, Gargiulo M, Pasquinelli G.

(12) Locally delivered adjuvant biofilm-penetrating antibiotics rescue impaired endochondral fracture healing caused by MRSA infectionJournal of orthopaedic research : official publication of the Orthopaedic Research SocietyFebruary 1, 2021Cahill SV, Kwon HK, Back J, Lee I, Lee S, Alder KD, Hao Z, Yu KE, Dussik CM, Kyriakides TR, et al.

(13) The Role of Panx3 in Age-Associated and Injury-Induced Intervertebral Disc DegenerationInternational journal of molecular sciencesJanuary 22, 2021Serjeant M, Moon PM, Quinonez D, Penuela S, Beier F, Séguin CA.IL, USA; 18165-1-AP); rabbit polyclonal anti-RUNX2 (1:100; Novus Biologicals, Centennial, CO, USA; NBP1-77461). As negative control, slides were incubated overnight in blocking solution in the absence of primary antibody

(14) Smooth Muscle Cell Reprogramming in Aortic AneurysmsCell stem cellApril 2, 2020Chen PY, Qin L, Li G, Malagon-Lopez J, Wang Z, Bergaya S, Gujja S, Caulk AW, Murtada SI, Zhang X, et al.3E10),Active Motif,"Cat# 61083, RRID:AB_2687450" Rabbit polyclonal anti-Runx2,NOVUS BIOLOGICALS,"Cat# NBP1-77461, RRID:AB_11003000" Rat monoclonal Anti-Ly-6A/E (Clone E13-161.7),BD Biosciences,"Cat# 553333, RRID:AB_394789" "Rabbit

(15) Rotator Cuff Tenocytes Differentiate into Hypertrophic Chondrocyte-Like Cells to Produce Calcium Deposits in an Alkaline Phosphatase-Dependent MannerJournal of clinical medicineSeptember 26, 2019Darrieutort-Laffite C, Arnolfo P, Garraud T, Adrait A, Couté Y, Louarn G, Trichet V, Layrolle P, Le Goff B, Blanchard F.of the samples. Sections were incubated with primary antibodies targeting RUNX2 (Novus Biologicals NBP1-77461, 1/200), SOX9 (ab3697, 1/400, Abcam, UK), Type II collagen (COL2A1) (sc-52658, 1/1000, Santa Cruz Biotechnology,

(16) Rapid fabrication of vascularized and innervated cell-laden bone models with biomimetic intrafibrillar collagen mineralizationNature communicationsAugust 6, 2019Thrivikraman G, Athirasala A, Gordon R, Zhang L, Bergan R, Keene DR, Jones JM, Xie H, Chen Z, Tao J, et al.anti-CD31 (Dako, JC70A) (1:200 dilution), rabbit polyclonal anti-RUNX2/CBFA1 antibody (Novus Biologicals, NBP1-77461) (1:100 dilution), mouse monoclonal anti-αSMA (Invitrogen, MA5-11547) (1:400 dilution), mouse monoclonal

(17) Human proximal tubular cells can form calcium phosphate deposits in osteogenic culture: role of cell death and osteoblast-like transdifferentiationCell death discoveryJanuary 1, 2019Priante G, Ceol M, Gianesello L, Furlan C, Del Prete D, Anglani F.(Asp214),D64E10,rabbit,Cell Signaling Technology,CST-5625,1:1000 Runx2/CBFA1,—,rabbit,Novus Biologicals,NBP1-77461,1:50 β-Tubulin,H235,rabbit,Santa Cruz Biotechnology,sc-9104,1:50 Primary antibodies and dilutions

(18) Caspase-independent programmed cell death triggers Ca₂PO₄ deposition in an in vitro model of nephrocalcinosisBioscience reportsFebruary 28, 2018Priante G, Quaggio F, Gianesello L, Ceol M, Cristofaro R, Terrin L, Furlan C, Del Prete D, Anglani F.) D64E10 Rabbit Cell Signaling Technology CST-5625 1:1000 Runx2/CBFA1 - Rabbit Novus Biologicals NBP1-77461 1:50 β-tubulin H235 Rabbit Santa Cruz Biotechnology sc-9104 1:50 Abbreviation: PARP, poly-(ADP-ribose)

(19) Cardiac Fibroblasts Adopt Osteogenic Fates and Can Be Targeted to Attenuate Pathological Heart CalcificationCell stem cellFebruary 2, 2017Pillai ICL, Li S, Romay M, Lam L, Lu Y, Huang J, Dillard N, Zemanova M, Rubbi L, Wang Y, et al.normal goat serum for 1 hour and then stained with primary antibodies against Runx2 (Novus biologicals, NBP1–77461), Osteocalcin (Abcam, ab 93876), Osterix (Santacruz, sc 22536-R), CD146 (Abcam, ab75769), NG2 (EMD Millipore,

(20) Deterministic encapsulation of single cells in thin tunable microgels for niche modelling and therapeutic deliveryNature materialsFebruary 1, 2017Mao AS, Shin JW, Utech S, Wang H, Uzun O, Li W, Cooper M, Hu Y, Zhang L, Weitz DA, et al.

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