Immunohistochemical (formalin-fixed, paraffin-embedded) staining of Human Hippocampus tissue using Product # PA5-19819, anti-Vasopressin antibody. Primary antibody was used at a concentration of 1 µg/mL and exposed for 15 mins at room temp. The sample was pretreated using heat mediated antigen retrieval with Sodium Citrate Buffer (pH6/20mins). The detection method was a HRP conjugated polymer, DAB chromogen and the sample was counterstained with haematoxylin and mounted with DPX.