RIP3 (B-2) Antibody sc-374639 from Santa Cruz Biotechnology, Inc.

Product Specs
ItemRIP3 (B-2) Antibody
CompanySanta Cruz Biotechnology, Inc.
Price $316.00Supplier Page View Company Product Page
Catalog Numbersc-374639
Quantity200 µg/ml
Reactivitymouse, rat, human
Antibody TypePrimary Antibodies
Hostmouse
ApplicationsWB, IP, IF, IHC(P), ELISA
Conjugate/TagUnconjugated
ClonalityMonoclonal
CloneB-2
NCBI Gene AliasesRIP3, Rip3, 2610528K09Rik
NCBI Full Gene Namereceptor interacting serine/threonine kinase 3, receptor-interacting serine-threonine kinase 3
TargetRIP3
FormatPurified
IsotypeIgG1

Questions and Answers

Can this RIP3 antibody be used to study necroptosis in various cell types?

Yes, the RIP3 (B-2) antibody has been utilized in studies investigating necroptosis, as evidenced by its application in flow cytometric detection of necroptotic cells in Jurkat cells, where it was shown to upregulate RIP3 during necroptosis induction (). Additionally, it has been referenced in studies examining necroptosis in macrophages and its role in various diseases ().

Is this antibody effective for Western blotting applications?

The RIP3 (B-2) antibody has been successfully used in Western blotting, as indicated by its application in multiple studies, including those examining the phosphorylation states of RIPK3 and its interactions with other proteins involved in necroptosis (, ). It has been cited in protocols requiring specific detection of RIP3 in various experimental contexts.

What species can this antibody react with, and is it suitable for human studies?

The RIP3 (B-2) antibody is reactive with mouse, rat, and human species, making it suitable for studies involving human samples, as indicated by its use in research involving human cell lines and tissues (, ).

Are there any specific applications in which this antibody has been shown to be particularly useful?

The RIP3 (B-2) antibody has been shown to work effectively in applications such as immunofluorescence and immunohistochemistry, as demonstrated in studies investigating the role of RIP3 in necroptosis and its implications in various diseases, including cancer and inflammatory conditions (, ).

Santa Cruz Biotechnology, Inc.

10410 Finnell Street

Dallas, Texas 75220

United States

Phone: 1-800-457-3801
831-457-3801

Company Profile

Website: http://www.scbt.com/

(1) Arsenic-Induced PPARγ, with the Coordinated Action of p62, Inhibits Apoptosis and Necroptosis and Activates the DNA Damage Response in A549 Lung Cancer Cells, Leading to CarcinogenesisCellsApril 8, 2026Hak-Ryul Kim, Seon-Hee Ohfrom Sigma-Aldrich (St. Louis, MO, USA). SQSTM1/p62 (sc-25575), Fas (sc-7886), p53 (sc-126), RIP3 (sc-374639), p21 (sc-6246), and K48-ubiquitin (sc-8017), as well as rhodamine-conjugated goat anti-rabbit (sc-2091)

(2) NLK facilitates Caspase-8 activation to drive macrophage PANoptosis in sepsisClin Transl MedFebruary 11, 2026Yun Xia, Ren-Qi Yao, Hui-Fan Liu, Guo-Qing Jing, Delida Aidebaike, Jing Zuo, Shi-Qi Wan, Die Wu, Peng-Yue Zhao, Hai-Long Gong, et al.and IgG (B900620) were purchased from Proteintech Group, Inc. (Wuhan, China). NLK (sc‐48361), RIPK3 (sc‐374639), and ASC (sc‐514414) antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

(3) Ginseng Polysaccharides Protect Against Endoplasmic Reticulum Stress-Induced Damage via PI3K/Akt Signalling Pathway in Bovine Ovarian Granulosa CellsCellsJanuary 17, 2026Hongjie Wang, Yi Fang, Lei Huang, Xu Yang, Xin Ma, Yang Lyu, Guo Jing, He Ding, Hongyu Liu, Wenfa LyuCat#: 70-306), anti-Receptor-Interacting Protein Kinase 3 (RIPK3) (Santa Cruz, Dallas, TX, USA, Cat#: Sc-374639), anti-Mixed-Lineage Kinase Domain-Like Protein (MLKL) (Proteintech, Cat#: 66675-1-1g), and anti-β-Actin

(4) Inflammation-driven biomimetic nano-polyphenol drug delivery system alleviates severe acute pancreatitis by inhibiting macrophage PANoptosis and pancreatic enzymes oversecretionJournal of Advanced ResearchJanuary 1, 2026Junyong Wu, Hai Huang, Wenjie Xu, Beibei Cui, Pengcheng Sun, XinYan Hao, Shihe Jiang, Xuyang Hou, Xiaoyan Qi, Zuxing Wei, et al.CST, USA), Ly6G (cat. BP0075-1, Biocell, USA), GOLPH3 (cat. 67777-1-Ig, Proteintech, USA), RIPK3 (cat. sc-374639, Santa Cruz, USA), Amylase (cat. sc-46657, Santa Cruz, USA). The following day, tissue sections and

(5) Necroptotic signaling orchestrates glioblastoma malignancy and potentiates temozolomide responseCell Death DisDecember 22, 2025Yuanyuan Li, Yuxin Qiu, Wenqing Gao, Lu Geng, Zerui Wu, Teng Zhang, Jiasong Pan, Jianglong Lu, Danfeng Zhang, Adi Idris, et al.temperature and incubated in primary antibodies against RIPK1 (Santa Cruz, sc133102), RIPK3 (Santa Cruz, sc374639), MLKL (Abcam, ab184718), p-MLKL (Abcam, ab187091), E-Cadherin (Beyotime, AF0138), N-Cadherin (Beyotime,

(6) Autophagy-Induced Microglial Death Contributes to Neuroinflammation in Acute Optic Nerve InjuryInvest Ophthalmol Vis SciDecember 16, 2025Shuping Ke, Jiajian Liang, Wai Kit Chu, Yan Tong, Suyao Wu, Weiqing Ye, Shaolang Chen, Qingping Liu, Dingguo Huang, Weiqi Chen, et al.lysates were prepared and incubated overnight at 4°C with either an antibody targeting RIP3 (25 µg, sc-374639; Santa Cruz Biotechnology) or a Mouse IgG1 Isotype Control (25 µg, 10400C; Thermo Fisher Scientific).

(7) Transition between cell states of sensitivity reveals molecular vulnerability of drug-tolerant cellsMolecular Systems BiologyOctober 1, 2025Ludovic Peyre, Marielle Péré, Mickael Meyer, Benjamin Bian, Marina Moureau-Barbato, Walid Djema, Bernard Mari, Georges Vassaux, Jérémie Roux(CST-14993) were obtained from Cell Signaling Technology, Inc. The mouse monoclonal antibody RIPK3 (sc-374639) from Santa Cruz Biotechnology, and FLIP (NF6) from Bio-Techne. The mouse monoclonal antibody GAPDH

(8) Mycobacterium tuberculosis, Mycobacterium kansasii and Rhodococcus equi induce macrophage necroptosis in the presence of a caspase inhibitor acting on a non-canonical target(s)bioRxivSeptember 11, 2025Rong Hu, Pei Li, Jie Han, Jiamin Huang, Guoyi Huang, Xiuju Jiang, Daniel Pfau, Guobao Li, Yi-Nan Gong, Carl F. Nathan, et al.(MAR1–5A3; #I-1188) were purchased from Leinco Technologies. Antibodies for β-actin (#sc-47778) and RIPK3 (#sc-374639) were from Santa Cruz Biotechnology. Antibodies for phospho-RIP1 (Ser166; #31122S), MLKL (#37705S for

(9) MLKL Modulates Necroptosis and Neuroinflammation in a Mouse Model of MSInflammationJuly 2, 2025Minjun Xiao, Ge Gao, Jingjing Mu, Qi Sun, Yun Zhao, Xueli FanMLKL (Proteintech Group, 66675–1-Ig, Monoclonal, Mouse/IgG1, China), RIPK3 (Santa Cruz Biotechnology, sc-374639, Monoclonal, Mouse/IgG1 κ, China), NLRP3 (Proteintech Group, 68102–1-Ig, Monoclonal, Mouse/IgG2a, China),

(10) Tumor Integrin-Targeted Glucose Oxidase Enzyme Promotes ROS-Mediated Cell Death that Combines with Interferon Alpha Therapy for Tumor ControlMolecular Cancer TherapeuticsJanuary 2, 2025Jordan A. Stinson, Allison Sheen, Brianna M. Lax, Grace N. Yang, Lauren Duhamel, Luciano Santollani, Elizabeth Fink, Joseph R. Palmeri, Karl Dane Wittrup(BD Cytofix/Cytoperm kit) similar to prior reports(47). Antibodies against RIP3 kinase (Santa Cruz sc-374639; Alexafluor-488; RRID:AB_10992232) and activated caspase 3 (BD 560626; Alexafluor-647; RRID:AB_1727414)

(11) Suppression of ZBP1-mediated NLRP3 inflammasome by the tegument protein VP22 facilitates pseudorabies virus infectionmBioDecember 11, 2024Zicheng Ma, Depeng Liu, Wandi Cao, Lei Guo, Kesen Liu, Juan Bai, Xingyi Li, Ping Jiang, Xing LiuSanta Cruz Biotechnology, sc-271483), anti-RIPK3 (Cell Signaling Technology, #95702; Santa Cruz Biotechnology, sc-374639), anti-Caspase-8 (Cell Signaling Technology, #4790; Santa Cruz Biotechnology, sc-81656), anti-Caspase-1

(12) Porcine parvovirus infection induces necroptosis of porcine placental trophoblast cells via a ZBP1-mediated pathwayVeterinary ResearchNovember 29, 2024Ning Xu, Qian Du, Yijiao Cheng, Lichen Nie, Peipei Ma, Dingwen Feng, Yong Huang, Dewen Tong(Beijing, China). Mouse anti-ZBP1 monoclonal antibody (sc-271483) and mouse anti-RIPK3 monoclonal antibody (sc-374639) were purchased from Santa Cruz (USA). Rabbit anti-p-RIPK3 monoclonal antibody (93654S) was purchased

(13) Different amyloid β42 preparations induce different cell death pathways in the model of SH-SY5Y neuroblastoma cellsCellular & Molecular Biology LettersNovember 17, 2024Alp Yigit Özdemir, Kateřina Hofbauerová, Vladimír Kopecký, Jiří Novotný, Vladimír Rudajevspectrin α II (sc-48382), HSP70 (sc-1060), HSP90 (sc-13119), HSP27 (sc-101699), MLKL (sc-293201), RIP3 (sc-374639), β-amyloid antibody (B-4) (sc-28365), rabbit anti-mouse IgG-HRP (sc-358914), mouse anti-rabbit IgG-HRP

(14) Protective role of cytosolic prion protein against virus infection in prion-infected cellsJournal of virologySeptember 17, 2024Hara H, Chida J, Batchuluun B, Takahashi E, Kido H, Sakaguchi S.mouse anti-phosphorylated-MLKL (Ser345) Ab (MABC1158; Millipore, Burlington MA), mouse anti-RIP3 Ab (sc-374639; Santa Cruz Biothechnology, Dallas, TX), rabbit anti-phosphorylated-RIP3 (Thr231, Ser232) Ab (ab222320;

(15) Wheel-Running Exercise Alleviates Anxiety-Like Behavior via Down-Regulating S-Nitrosylation of Gephyrin in the Basolateral Amygdala of Male RatsAdvanced science (Weinheim, Baden-Wurttemberg, Germany)September 1, 2024Yang PF, Nie TL, Sun XN, Xu LX, Ma C, Wang F, Long LH, Chen JG.610 584, BD, USA), Caspase‐3 (1:1000, #9661, Cell Signaling Technology, Danvers, USA), RIP3 (1:500, SC‐374639, Santa Cruz Biotechnology, California, USA), Bcl‐2 (1:200, SC‐7382, Santa Cruz Biotechnology, California,

(16) NLRC5 senses NAD(+) depletion, forming a PANoptosome and driving PANoptosis and inflammationCellJuly 25, 2024Sundaram B, Pandian N, Kim HJ, Abdelaal HM, Mall R, Indari O, Sarkar R, Tweedell RE, Alonzo EQ, Klein J, et al.AB_203256 anti-human NLRP12 (for IP),Abcepta,Cat# AP14014a; RRID:AB_10893749 anti-RIPK3 (for IP),Santa Cruz,Cat# sc-374639; RRID:AB_10332232 anti-Myc,Cell Signaling Technology,Cat# 2276; RRID:AB_331783 anti-NLRP3,AdipoGen,Cat#

(17) Exploring cell death mechanisms in spheroid cultures using a novel application of the RIP3-caspase3-assayScientific ReportsJuly 11, 2024C. I. Philippi, J. Hagens, K. M. Heuer, H. C. Schmidt, P. Schuppert, L. Pagerols Raluy, M. Trochimiuk, Z. Li, M. J. Bunders, K. Reinshagen, et al.(#564096, dilution 1:50; BD Biosciences, San Jose, CA, USA) and anti-RIP3-Alexa Fluor 488 (clone B-2, #sc-374639, dilution 1:125, Santa Cruz Biotechnology, Dallas, TX, USA) for 30 min at 4 °C in the dark. Cells were

(18) ZBP1 condensate formation synergizes Z-NAs recognition and signal transductionCell Death & DiseaseJuly 9, 2024Feiyan Xie, Di Wu, Jing Huang, Xuehe Liu, Yanfang Shen, Jinqing Huang, Zhipeng Su, Jixi Lisupernatant were separated by SDS-PAGE, and incubated with specific antibodies: anti-RIP3 (Santa Cruz, sc-374639), anti-MLKL (Abcam, ab184718), anti-p-RIPK3 (Abcam, ab209384), anti-p-MLKL (Abcam, ab187091), anti-GFP

(19) MLKL, a new actor of UVB-induced apoptosis in human diploid dermal fibroblastsCell Death DiscoveryMay 14, 2024Anne-Sophie Gary, Sophie Amouret, Alicia Montoni, Patrick J. RochetteUSA), α-PARP (F2, #sc-8007, 200 ug/mL, 1:500, Santa Cruz Biotechnology, Dallas, TX, USA), α-RIPK3 (B2, Sc-374639, 1:1000, Santa Cruz Biotechnology) or α-RIPK3 (6E6.2, #MABC28, 1:1000, Millipore, Oakville, CA) and

(20) Local hyperthermia mediated by gold nanoparticle-integrated silicone-covered stent: feasibility and tissue response in a rat esophageal modelEuropean Radiology ExperimentalApril 3, 2024Jiaywei Tsauo, Yue Liu, Xiaowu Zhang, Yan Fu, He Zhao, Tao Gong, Jingui Li, Xiao LiQbiogene, Darmstadt, Germany) and a primary antibody against Receptor-interacting kinase 3 (RIP3) (sc-374639; Santa Cruz Biotechnology, CA, USA; diluted 1:50). After staining, sections were examined using a digital

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RIP3 (B-2) Antibody

RIP3 (B-2) Antibody from Santa Cruz Biotechnology, Inc.

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Santa Cruz Biotechnology, Inc.

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RIP3 (B-2) Antibody from Santa Cruz Biotechnology, Inc.

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