Western blot was performed using Anti-FABP4 Polyclonal Antibody (Product # PA5-17248) and a 15 kDa band corresponding to Fatty acid-binding protein, adipocyte was observed across cell lines and tissues. Whole cell extracts (40 µg lysate) of 3T3-L1 (Lane 1), 3T3-L1 differentiated to adipocytes (Lane 2), Mouse Adipose (Lane 3), Rat Adipose (Lane 4), Mouse Brain (Lane 5), Rat Brain (Lane 6) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0341BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:20000 dilution using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076). Upon differentiation of 3T3-L1 cells to adipocytes the expression of FABP4 should upregulate as observed. Also, a relative expression was observed in Adipose (high expressing tissue model) versus brain (low expressing tissue model) as reported.