Fig 1: K414R mutation inhibits lipolysis. K414R lentivirus was used to infect 3T3‐L1 mature adipocytes. Following treatment with ISO 1 µM for 30 min and TNF‐α 50 ng/mL for 3 h, a decrease in the content of non‐esterified fatty acids (NEFA) and glycerol in the culture medium of the K414R group was observed (A‐B). K414R lentivirus was used to infect 3T3‐L1 mature adipocytes. Following treatment with ISO 1 µM for 30 min and TNF‐α 50 ng/mL for 3 h, ELISA showed a decrease in the content of leptin (C) and an increase in adiponectin content (D) in the culture medium of the K414R group. K414R lentivirus was used to infect 3T3‐L1 mature adipocytes. Western blot showed that the expression of fatty acid synthase (FAS) was increased, and the expression of the lipolytic enzymes HSL, p‐HSL and ATG was decreased, and the expression of perilipin was increased (E). Data are shown as the means ±standard deviations of three independent experiments performed in triplicate. *** P < .001