TMPRSS2 Antibody PA5-14264 from Thermo Fisher Scientific

Product Specs
ItemTMPRSS2 Antibody
CompanyThermo Fisher Scientific
Price Supplier Page
Catalog NumberPA5-14264
Quantity400 µL
Conjugate/TagUnconjugated
HostRabbit
TargetTMPRSS2
ApplicationsFlow Cytometry, Immunohistochemistry (Paraffin), Western Blot
FormatPBS with 0.09% sodium azide; pH 7.4
ReactivityHuman
NCBI Gene AliasesPRSS10
ClonalityPolyclonal
NCBI Full Gene Nametransmembrane serine protease 2
Concentration2.0 mg/mL
ImmunogenKLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human TMPRSS2
IsotypeIgG
Storage Temperature-20° C, Avoid Freeze/Thaw Cycles
PurityAmmonium sulfate precipitation, Size-exclusion - Dialysis
Antibody TypePrimary
StorageStore at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles

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Thermo Fisher Scientific

81 Wyman Street

Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

Company Profile

Website: www.thermofisher.com

(1) A pH-driven receptor switch from ACE2 to NPC1 enables endosomal entry of SARS-CoV-2ResearchSquareMarch 4, 2026Yong-Hui Zheng, Zhongshun Liu, Yu Pang, Ilyas Khan, Li Ou, Yixiang Hu, Sunan Li, Justin Richner, Tongqing Zhou1:1000 for Western blotting) were obtained from Proteintech. Rabbit polyclonal anti-TMPRSS2 (Cat. No. PA5-14264; 1:50 for flow cytometry) was from Invitrogen. Goat anti-human ACE2 affinity-purified IgG (Cat. No.

(2) High-Fidelity and Cost-Effective Engineering of SARS-CoV-2VirusesDecember 11, 2025Marco Olguin-Nava, Thomas Hennig, Charlene Börtlein, Patrick Bohn, Uddhav B. Ambi, Alexander Gabel, Lina M. Günter, Anne-Sophie Gribling-Burrer, Nora Schmidt, Neva Caliskan, et al.Danvers, MA, USA), anti-ACE2 (cat. 66699, Proteintech Group, Rosemont, IL, USA), anti-TMPRSS2 (cat. PA5-14264, Thermo Fisher Scientific, Waltham, MA, USA), or anti-Actin (cat. sc-47778, Santa Cruz Biotechnology,

(3) Spike substitutions E484D, P812R and Q954H mediate ACE2-independent entry of SARS-CoV-2 across different cell linesPloS oneJanuary 1, 2025Vizgirda G, Underwood AP, Fahnøe U, Weis N, Ramirez S, Bukh J.0.01 µg/ml for 2 h. For blocking of TMPRSS2, an anti-TMPRSS2 antibody (Thermo Fisher Scientific, Cat#PA5−14264) was added to the cells in quadruplicate at 50 µg/ml, 25 µg/ml, 12.5 µg/ml, 6.25 µg/ml and 3.125 µg/ml.

(4) Uptake of severe acute respiratory syndrome coronavirus 2 spike protein mediated by angiotensin converting enzyme 2 and ganglioside in human cerebrovascular cellsFrontiers in neuroscienceJanuary 1, 2023McQuaid C, Solorzano A, Dickerson I, Deane R.used at a low (10 μg/ml) and high (60 μg/ml) concentration; anti-TMPRSSE2 antibody (Invitrogen, Cat# PA5-14264) used at 13 μg/ml; anti-CD147 antibody (Invitrogen, Cat# 34-5600) used at 2.5 μg/ml; anti-NP-1 antibody

(5) Highly restricted SARS-CoV-2 receptor expression and resistance to infection by primary human monocytes and monocyte-derived macrophagesJournal of leukocyte biologySeptember 1, 2022Zankharia U, Yadav A, Yi Y, Hahn BH, Collman RG.554714), and incubated with polyclonal anti‐TMPRSS2 primary antibody (rabbit anti‐hu TMPRSS2; cat# PA5‐14264; Invitrogen) diluted 1:25 for 30 min in the dark at 4°C. Cells were washed and APC‐conjugated secondary

(6) Peptidomimetic inhibitors of TMPRSS2 block SARS-CoV-2 infection in cell cultureCommunications biologyJuly 8, 2022Wettstein L, Knaff PM, Kersten C, Müller P, Weil T, Conzelmann C, Müller JA, Brückner M, Hoffmann M, Pöhlmann S, et al.phosphate-buffered saline (DPBS, Sigma-Aldrich) and incubated with the TMPRSS2 antibody (ThermoFisher Scientific, PA5-14264) at final concentrations of 10, 20, 40, and 100 µg/mL for 30 min at 4 °C. After the separation from

(7) Antiviral Potential of the Antimicrobial Drug Atovaquone against SARS-CoV-2 and Emerging Variants of ConcernACS infectious diseasesNovember 12, 2021Carter-Timofte ME, Arulanandam R, Kurmasheva N, Fu K, Laroche G, Taha Z, van der Horst D, Cassin L, van der Sluis RM, Palermo E, et al.clone AC384, Novus Biologicals Cat#NBP2-80038PCP, or unconjugated rabbit antihuman TMPRSS2, Invitrogen PA5-14264). Cells were then washed twice, and for ACE2 analysis, they were fixated using 1% formaldehyde (Avantor,

(8) Polyphenylene carboxymethylene (PPCM) microbicide repurposed as antiviral against SARS-CoV-2. Proof of concept in primary human undifferentiated epithelial cellsAntiviral ResearchOctober 1, 2021Olivier Escaffre, Alexander N. Freibergused in this study were rabbit anti-ACE2 (Invitrogen, #MA5-32307, d/500), anti-TMPRSS2 (Invitrogen, #PA5-14264, d/5000) and anti-actin (Cell signaling, clone 13E5, d/5000). Stripping (2% SDS, 0.05 M Tris-HCl, 1%

(9) Expression of SARS-CoV-2-related receptors in cells of the neurovascular unit: implications for HIV-1 infectionJournal of neuroinflammationJuly 29, 2021Torices S, Cabrera R, Stangis M, Naranjo O, Fattakhov N, Teglas T, Adesse D, Toborek M.anti-claudin-5 (Thermo Fisher Scientific, Cat#341600) and rabbit anti-TMPRSS2 (Thermo Fisher Scientific, Cat# PA5-14264). Membranes were imaged using the Licor CLX imaging system after incubation with the secondary antibody

(10) IMMUNO-COV v2.0: Development and Validation of a High-Throughput Clinical Assay for Measuring SARS-CoV-2-Neutralizing Antibody TitersmSphereJune 30, 2021Vandergaast R, Carey T, Reiter S, Lathrum C, Lech P, Gnanadurai C, Haselton M, Buehler J, Narjari R, Schnebeck L, et al.resuspended in 100 μl of a 0.5% saponin solution containing 4 μg rabbit anti-TMPRSS2 (Invitrogen catalog no. PA5-14264). After 30 min on ice, samples were washed twice with 1 ml FACS buffer and resuspended in 100 μl of

(11) A high-throughput screen for TMPRSS2 expression identifies FDA-approved compounds that can limit SARS-CoV-2 entryNature communicationsJune 23, 2021Chen Y, Lear TB, Evankovich JW, Larsen MB, Lin B, Alfaras I, Kennerdell JR, Salminen L, Camarco DP, Lockwood KC, et al.Triton-X-100 for 0.5 h, and blocked with Duolink Blocking solution at 37 °C for 2 h. Anti-TMPRSS2 (Thermo, PA5-14264) and anti-DCAF1 (Santa Cruz Biotechnology, (C-8, sc-376850) antibodies were incubated with cells overnight.

(12) Alpha-1 antitrypsin inhibits TMPRSS2 protease activity and SARS-CoV-2 infectionNature communicationsMarch 19, 2021Wettstein L, Weil T, Conzelmann C, Müller JA, Groß R, Hirschenberger M, Seidel A, Klute S, Zech F, Prelli Bozzo C, et al.of TMPRSS2 was verified using a TMPRSS2 antibody (rabbit anti-human IgG, ThermoFischer Scientific, PA5-14264) dissolved in HEPES buffer to a concentration of 0.2 mg/ml (1:10 of 2 mg/ml stock). As a negative control,

(13) Human Erythroid Progenitors Are Directly Infected by SARS-CoV-2: Implications for Emerging Erythropoiesis in Severe COVID-19 PatientsStem cell reportsMarch 9, 2021Huerga Encabo H, Grey W, Garcia-Albornoz M, Wood H, Ulferts R, Aramburu IV, Kulasekararaj AG, Mufti G, Papayannopoulos V, Beale R, et al.antibody (R&D Systems, Cat# AF933) at 15 μg/mL and polyclonal rabbit anti-TMPRSS2 (ThermoFisher, Cat# PA5-14264) at 15 μg/mL were used for the primary antibody incubation. Donkey anti-goat Alexa 647 (Invitrogen,

(14) Expression of SARS-CoV-2-related Receptors in Cells of the Neurovascular Unit: Implications for HIV-1 InfectionResearch squareFebruary 24, 2021Torices S, Cabrera R, Stangis M, Naranjo O, Adesse D, Toborek M.anti-claudin-5 (Thermo Fisher Scientific, Cat#341600) and rabbit anti-TMPRSS2 (Thermo Fisher Scientific, Cat# PA5–14264). Membranes were imaged using the Licor CLX imaging system after incubation with the secondary antibody

(15) Infection of bat and human intestinal organoids by SARS-CoV-2Nature medicineJuly 1, 2020Zhou J, Li C, Liu X, Chiu MC, Zhao X, Wang D, Wei Y, Lee A, Zhang AJ, Chu H, et al.secondary antibodies. Other antibodies used in the study included α-ACE2 (AF933, R&D Systems), α-TMPRSS2 (PA5-14264, Invitrogen), goat anti-rabbit IgG Alexa Fluor 594 (A-11037, Invitrogen) and donkey Table 3 | RT–PCR assay

(16) Development and validation of IMMUNO-COV™: a high-throughput clinical assay for detecting antibodies that neutralize SARS-CoV-2bioRxiv : the preprint server for biologyMay 27, 2020Vandergaast R, Carey T, Reiter S, Lech P, Gnanadurai C, Tesfay M, Buehler J, Suksanpaisan L, Naik S, Brunton B, et al.

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TMPRSS2 Polyclonal Antibody

TMPRSS2 Antibody from Thermo Fisher Scientific

Thermo Fisher Scientific

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TMPRSS2 Antibody from Thermo Fisher Scientific

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