MBP Antibody (12)

Product Specs
ItemMBP Antibody (12) - BSA Free
CompanyNovus Biologicals, a Bio-Techne Brand
Price $419.00Supplier Page View Company Product Page
Catalog NumberNB600-717
ImmunogenBovine MBP
Conjugate/TagUnconjugated
ApplicationsIF/IHC,ELISA,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Radioimmunodiffusion,Radioimmunoassay,Immunocytochemistry/ Immunofluorescence
Clone12
Format0.1 M Tris
TargetMBP
HostRat
ReactivityBovine,Canine,Feline,Guinea Pig,Human,Mouse,Primate,Rat
Quantity1 ml (also 0.25 ml)
IsotypeIgG2a
ClonalityMonoclonal
PurityTissue culture supernatant
Research AreaNeuroscience, Neurotransmission, Oligodendrocyte Cell Markers, Stem Cell Markers
Antibody TypePrimary Antibodies
StorageStore at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles
NCBI Full Gene Namemyelin basic protein

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: NB600-717 reacts with myelin basic protein from a wide range of species. The antibody reacts weakly with peptides ending in the Phe 91 where the 91-92 Phe-Phe bond is broken. Synthetic peptide 82-99 reacts very well, as does intact MBP. Further epitope analysis indicates binding to a region defined by amino acids 82-87 (DENPVV). Clone 12 has been reported as being suitable for use in Western blotting.

Sizes Available: 1 ml (also 0.25 ml)

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Questions and Answers

Can this antibody be used to study myelination in mouse models of multiple sclerosis?

Yes, the MBP antibody has been utilized in studies involving cuprizone-induced mouse models of multiple sclerosis to assess myelination scores. In one study, the antibody was used for immunohistochemistry to evaluate myelin basic protein expression during demyelination and remyelination phases ().

Is this antibody effective for use in immunofluorescence applications?

The MBP antibody has been shown to work in immunofluorescence applications, as indicated by its use in various studies to visualize myelin basic protein in different species, including mice and rats (, ).

What species can this antibody react with, and is it suitable for cross-species studies?

The MBP antibody is reactive with a wide range of species, including humans, mice, rats, dogs, and non-human primates. This broad reactivity makes it suitable for cross-species studies in neuroscience research (, ).

Has this antibody been cited in research related to oligodendrocyte function?

Yes, the MBP antibody has been cited in multiple studies focusing on oligodendrocyte function and myelination processes, including investigations into the role of oligodendrocyte progenitor cells and their differentiation in various neurological contexts (, ).

Novus Biologicals, a Bio-Techne Brand

United States: 10771 E Easter Ave

Centennial, Colorado 80112

United States

Phone:
303-730-1950
888-506-6887

Email: novus@novusbio.com

Company Profile

Website: https://www.novusbio.com

(1) Acute Intermittent Hypoxia Exerts Beneficial Effects and Promotes Repair in Male Mice in the Experimental Autoimmune Encephalomyelitis Model of Multiple SclerosisGliaDecember 28, 2025Nataliya Tokarska, Josh Roberts, Sameer Rathnayaka Koralage, Justin M. A. Naniong, Jayne M. Johnston, Zelan Wei, Valerie M. K. Vergenitric oxide synthase (iNOS; Sigma‐Aldrich #N7782; 1:500), rat anti‐myelin basic protein (MBP; NovusBio #NB600‐717; 1:20), goat anti‐platelet derived growth factor receptor alpha (PDGFRα; R&D Systems #AF1062; 1:75),

(2) A mouse organoid platform for modeling cerebral cortex development and cis-regulatory evolution in vitroDevelopmental CellDecember 15, 2025Daniel Medina-Cano, Mohammed T. Islam, Veronika Petrova, Sanjana Dixit, Zerina Balic, Marty G. Yang, Matthias Stadtfeld, Emily S. Wong, Thomas Vierbuchenanti-POU3F3/BRN1,Novus Biologicals,Cat#NBP1-49872;RRID:AB_10012062 Rat mAb anti-MBP (Clone 12),Novus Biologicals,Cat#NB600-717;RRID:AB_2139899 Rabbit mAb anti-S100β (EP1576Y),Abcam,Cat#AB52642;RRID:AB_882426 Goat pAb anti-PDGFRα,R&D Systems

(3) Necl-4/CADM4 regulates GABAergic synaptic strength on GABAergic inhibitory neurons via ErbB4 activation and prevents neuronal impairmentsMolecular neurobiologyDecember 1, 2025Komaki R, Shiotani H, Kuriu T, Kameyama T, Miyata M, Kedashiro S, Katanazaka K, Nishii S, Chihara N, Matsumoto R, et al.(Synaptic Systems, 160 011), chicken anti-MAP2 pAb (Abcam, ab5392), rat anti-MBP mAb (Novus Biologicals, NB600-717), mouse anti-Necl-4 mAb (NeuroMab, 75–247), goat anti-PV pAb (FRONTIER INSTITUTE, PV-Go-Af460), Cy3-conjugated

(4) Five-year analysis of efficacy and safety of a bidirectional AAV gene therapy in Tay-Sachs sheepJournal of Clinical InvestigationDecember 1, 2025Toloo Taghian, Jillian Gallagher, Stephanie Bertrand, William C. Baker, Kalajan Lopez Mercado, Hector R. Benatti, Erin Hall, Yvette Lopez, Abigail McElroy, John T. McCarthy, et al.immunofluorescence included MAP2 (CH22103, Neuromics), recombinant anti-Olig2 antibody (ab109186, Abcam), MBP (NB600-717, Novus Biologicals), calcium-binding adapter molecule 1 (CP290, Biocare), and glial fibrillary acidic

(5) Five-year analysis of efficacy and safety of a bidirectional AAV gene therapy in Tay-Sachs sheepJ Clin InvestSeptember 30, 2025Toloo Taghian, Jillian Gallagher, Stephanie Bertrand, William C. Baker, Kalajan Lopez Mercado, Hector R. Benatti, Erin Hall, Yvette Lopez, Abigail McElroy, John T. McCarthy, et al.immunofluorescence included MAP2 (CH22103, Neuromics), recombinant anti-Olig2 antibody (ab109186, Abcam), MBP (NB600-717, Novus Biologicals), calcium-binding adapter molecule 1 (CP290, Biocare), and glial fibrillary acidic

(6) Laminar organization of the anterior olfactory nucleus—the interplay between neurogenesis timing and neuroblast migrationFrontiers in NeuroscienceApril 30, 2025Eduardo Martin-Lopez, Bowen Brennan, Marion Lefèvre, Natalie J. Spence, Kimberly Han, Charles A. Greer555,Thermo Fisher Scientific,"1:1,000" Myelin basic protein (MBP),Rat IgG2a clone 12,Novus Biologicals (nb600-717),AB_2139899,1:500,Goat α-rat IgG Alexa 488,Thermo Fisher Scientific,"1:1,000" NeuroD1,Mouse IgG2a,Abcam

(7) The X-Linked Intellectual Disability Gene, ZDHHC9 , Is Important for Oligodendrocyte Subtype Determination and MyelinationGliaMarch 19, 2025Rocio B. White, Angela R. Wild, Timothy P. O'Leary, Andrew J. Thompson, Stephane Flibotte, Angie Peng, Jason C. Rogalski, Mila Mair, Neeki Derhami, Shernaz X. Bamjiantibodies overnight at room temperature (MAG: 1:1000 dilution, 34–62,000, Invitrogen; MBP: 1:1000 dilution, NB600‐717, Novus bio; MOBP: 1:1000 dilution, #PA5‐100618, Invitrogen; CLDN11: 1:1000, 36–4500, Invitrogen; MOG:

(8) Anti-RGMa neutralizing antibody ameliorates vascular cognitive impairment in miceNeurotherapeuticsMarch 1, 2025Masaya Yamamoto, Takahide Itokazu, Hiroki Uno, Takakuni Maki, Nao Shibuya, Toshihide Yamashitaanti-Sox2 antibody (1:500; AB5603, Millipore), rat anti-myelin basic protein (MBP) antibody (1:200, NB600-717, NOVUS), rabbit anti-Cleaved caspase-3 antibody (1:1000; 9661, Cell signaling), and Alexa Fluor 647

(9) Characterizing and targeting glioblastoma neuron-tumor networks with retrograde tracingCellJanuary 23, 2025Tetzlaff SK, Reyhan E, Layer N, Bengtson CP, Heuer A, Schroers J, Faymonville AJ, Langeroudi AP, Drewa N, Keifert E, et al.

(10) Inhibiting the Cholesterol Storage Enzyme ACAT1/SOAT1 in Myelin Debris-Treated Microglial Cell Lines Activates the Gene Expression of Cholesterol Efflux Transporter ABCA1BiomoleculesOctober 14, 2024Thao N. Huynh, Matthew C. Havrda, George J. Zanazzi, Catherine C. Y. Chang, Ta Yuan Changanti-ACAT1 (DM102) produced in the Chang lab, anti-PLIN2 (Proteintech 15294-1-AP), anti-MBP (Novus NB600-717) and anti-vinculin (Millipore 05-386) as a protein loading control. Blots were then washed and incubated

(11) Control of OPC proliferation and repopulation by the intellectual disability gene PAK1 under homeostatic and demyelinating conditionsbioRxivMay 4, 2024Yan Wang, Bokyung Kim, Shuaishuai Gong, Joohyun Park, Meina Zhu, Evelyn M. Wong, Audrey Y. Park, Jonathan Chernoff, Fuzheng GuoNOVUS), P-PDGFRα(Y754) (1:1000, #ab5460, Abcam), P-PDGFRα(Y849) (1:1000, #ab79318, Abcam), MBP (1:1000, #NB600–717, Novus), ERK1/2 (1:1000, #9102, Cell Signaling Technology), P-ERK1/2 (Thr202/Tyr204) (1:1000, #9101,

(12) Bu Shen Huo Xue decoction promotes functional recovery in spinal cord injury mice by improving the microenvironment to promote axonal regenerationChinese medicineJuly 12, 2022Hou Y, Luo D, Hou Y, Luan J, Zhan J, Chen Z, E S, Xu L, Lin D.

(13) Krabbe disease successfully treated via monotherapy of intrathecal gene therapyJournal of Clinical InvestigationAugust 10, 2020Allison M. Bradbury, Jessica H. Bagel, Duc Nguyen, Erik A. Lykken, Jill Pesayco Salvador, Xuntian Jiang, Gary P. Swain, Charles A. Assenmacher, Ian J. Hendricks, Keiko Miyadera, et al.rabbit polyclonal anti-GALC (AB137750, Abcam; 1:200) and rat monoclonal anti–myelin basic protein (NB600717, Novus Biologics; 1:500) antibodies were diluted in antibody dilu- RESEARCH ARTICLE The Journal

(14) Sox2 Is Essential for Oligodendroglial Proliferation and Differentiation during Postnatal Brain Myelination and CNS RemyelinationThe Journal of NeuroscienceFebruary 14, 2018Sheng Zhang, Xiaoqing Zhu, Xuehong Gui, Christopher Croteau, Lanying Song, Jie Xu, Aijun Wang, Peter Bannerman, Fuzheng GuoRRID:AB_2199816,1:200; Cell Signaling Technology; sc8632, RRID:AB_2199825, 1:100; Santa Cruz Biotechnology), MBP (NB600717, RRID:AB_2139899, 1:200; Novus), SMI312 (SMI-312R, RRID: AB_2135329, 1:1000, Covance), active caspase-3

(15) ZPK/DLK and MKK4 Form the Critical Gateway to Axotomy-Induced Motoneuron Death in NeonatesJournal of NeuroscienceAugust 6, 2014Takayuki Itoh, Makoto Horiuchi, Raymond H Ikeda, Jie Xu, Peter Bannerman, David Pleasure, Josef M Penninger, Cathy Tournier, Aki Itoh#019-19741, RRID:AB_839504 Sigma-Aldrich catalog #N4142, RRID:AB_477272 Novus Biologicals catalog #NB600-717, RRID:AB_2139899 IB, Immunoblotting; IHC, immunohistochemistry. Figure 1. ZPK/DLK-deficient and CNS

(16) ZPK/DLK, a Mitogen-Activated Protein Kinase Kinase Kinase, Is a Critical Mediator of Programmed Cell Death of MotoneuronsThe Journal of NeuroscienceMay 18, 2011Aki Itoh, Makoto Horiuchi, Kouji Wakayama, Jie Xu, Peter Bannerman, David Pleasure, Takayuki Itohrabbit antiserum against anti-NF-200 (N4142, 1:500; Sigma), and rat monoclonal anti-myelin basic protein (MBP) (NB600 –717, 1:20; Novus Biologicals). Then, the sections were washed with PBS, incubated for 1 h in appropriate

(17) Krabbe disease successfully treated via monotherapy of intrathecal gene therapyThe Journal of clinical investigationJanuary 1, 0001"Bradbury AM, Bagel JH, Nguyen D, Lykken EA, Pesayco Salvador J, Jiang X, Swain GP, Assenmacher CA, Hendricks IJ, Miyadera K, et al."rabbit polyclonal anti-GALC (AB137750, Abcam; 1:200) and rat monoclonal anti–myelin basic protein (NB600717, Novus Biologics; 1:500) antibodies were diluted in antibody dilu- RESEARCH ARTICLE The Journal

(18) Microglia processes associate with diffusely injured axons following mild traumatic brain injury in the micro pigJournal of neuroinflammationJanuary 1, 0001"Lafrenaye AD, Todani M, Walker SA, Povlishock JT."Iba-1 (1:1000; Cat.# 019-19741, Wako, Osaka, Japan), and rat anti-myelin basic protein (1:1000; Cat#NB600-717, Novus Biologicals, Littleton, CO, USA). To reduce the amount of lipid within the tissue and enhance

(19) Combination HSCT and intravenous AAV-mediated gene therapy in a canine model proves pivotal for translation of Krabbe disease therapyMolecular therapy : the journal of the American Society of Gene TherapyJanuary 1, 0001"Bradbury AM, Bagel J, Swain G, Miyadera K, Pesayco JP, Assenmacher CA, Brisson B, Hendricks I, Wang XH, Herbst Z, et al."

(20) Pleiotrophin-Neuregulin1 promote axon regeneration and sorting in conduit repair of critical nerve gap injuriesResearch squareJanuary 1, 0001"Gu X, Rahman FS, Bendale G, Tran B, Miyata JF, Hernandez A, Anand S, Romero-Ortega MI."

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1/28/2020Excellent Myelin Basic Protein AntibodyDr. David SunnenA very sensitive myelin marker and generates robust fluorescence signals.

MBP Antibody (12)

MBP Antibody (12)

MBP Antibody (12)

MBP Antibody (12)

MBP Antibody (12) - BSA Free from Novus Biologicals, a Bio-Techne Brand

Specifications/Features

Questions and Answers

Novus Biologicals, a Bio-Techne Brand

Citations (61)

Reviews

MBP Antibody (12) - BSA Free from Novus Biologicals, a Bio-Techne Brand

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