MBP Antibody (12)

Product Specs
ItemMBP Antibody (12) - BSA Free
CompanyNovus Biologicals, a Bio-Techne Brand
Price $419.00Supplier Page View Company Product Page
Catalog NumberNB600-717
ImmunogenBovine MBP
Conjugate/TagUnconjugated
ApplicationsIF/IHC,ELISA,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Radioimmunodiffusion,Radioimmunoassay,Immunocytochemistry/ Immunofluorescence
Clone12
Format0.1 M Tris
TargetMBP
HostRat
ReactivityBovine,Canine,Feline,Guinea Pig,Human,Mouse,Primate,Rat
Quantity1 ml (also 0.25 ml)
IsotypeIgG2a
ClonalityMonoclonal
PurityTissue culture supernatant
Research AreaNeuroscience, Neurotransmission, Oligodendrocyte Cell Markers, Stem Cell Markers
Antibody TypePrimary Antibodies
StorageStore at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles
NCBI Full Gene Namemyelin basic protein

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: NB600-717 reacts with myelin basic protein from a wide range of species. The antibody reacts weakly with peptides ending in the Phe 91 where the 91-92 Phe-Phe bond is broken. Synthetic peptide 82-99 reacts very well, as does intact MBP. Further epitope analysis indicates binding to a region defined by amino acids 82-87 (DENPVV). Clone 12 has been reported as being suitable for use in Western blotting.

Sizes Available: 1 ml (also 0.25 ml)

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Questions and Answers

Can this antibody be used to study myelination in mouse models of multiple sclerosis?

Yes, the MBP antibody has been utilized in studies involving cuprizone-induced mouse models of multiple sclerosis to assess myelination scores. In one study, the antibody was used for immunohistochemistry to evaluate myelin basic protein expression during demyelination and remyelination phases ().

Is this antibody effective for use in immunofluorescence applications?

The MBP antibody has been shown to work in immunofluorescence applications, as indicated by its use in various studies to visualize myelin basic protein in different species, including mice and rats (, ).

What species can this antibody react with, and is it suitable for cross-species studies?

The MBP antibody is reactive with a wide range of species, including humans, mice, rats, dogs, and non-human primates. This broad reactivity makes it suitable for cross-species studies in neuroscience research (, ).

Has this antibody been cited in research related to oligodendrocyte function?

Yes, the MBP antibody has been cited in multiple studies focusing on oligodendrocyte function and myelination processes, including investigations into the role of oligodendrocyte progenitor cells and their differentiation in various neurological contexts (, ).

Novus Biologicals, a Bio-Techne Brand

United States: 10771 E Easter Ave

Centennial, Colorado 80112

United States

Phone:
303-730-1950
888-506-6887

Email: novus@novusbio.com

Company Profile

Website: https://www.novusbio.com

(1) Acute Intermittent Hypoxia Exerts Beneficial Effects and Promotes Repair in Male Mice in the Experimental Autoimmune Encephalomyelitis Model of Multiple SclerosisGliaDecember 28, 2025Nataliya Tokarska, Josh Roberts, Sameer Rathnayaka Koralage, Justin M. A. Naniong, Jayne M. Johnston, Zelan Wei, Valerie M. K. Vergenitric oxide synthase (iNOS; Sigma‐Aldrich #N7782; 1:500), rat anti‐myelin basic protein (MBP; NovusBio #NB600‐717; 1:20), goat anti‐platelet derived growth factor receptor alpha (PDGFRα; R&D Systems #AF1062; 1:75),

(2) A mouse organoid platform for modeling cerebral cortex development and cis-regulatory evolution in vitroDevelopmental CellDecember 15, 2025Daniel Medina-Cano, Mohammed T. Islam, Veronika Petrova, Sanjana Dixit, Zerina Balic, Marty G. Yang, Matthias Stadtfeld, Emily S. Wong, Thomas Vierbuchenanti-POU3F3/BRN1,Novus Biologicals,Cat#NBP1-49872;RRID:AB_10012062 Rat mAb anti-MBP (Clone 12),Novus Biologicals,Cat#NB600-717;RRID:AB_2139899 Rabbit mAb anti-S100β (EP1576Y),Abcam,Cat#AB52642;RRID:AB_882426 Goat pAb anti-PDGFRα,R&D Systems

(3) Five-year analysis of efficacy and safety of a bidirectional AAV gene therapy in Tay-Sachs sheepJournal of Clinical InvestigationDecember 1, 2025Toloo Taghian, Jillian Gallagher, Stephanie Bertrand, William C. Baker, Kalajan Lopez Mercado, Hector R. Benatti, Erin Hall, Yvette Lopez, Abigail McElroy, John T. McCarthy, et al.immunofluorescence included MAP2 (CH22103, Neuromics), recombinant anti-Olig2 antibody (ab109186, Abcam), MBP (NB600-717, Novus Biologicals), calcium-binding adapter molecule 1 (CP290, Biocare), and glial fibrillary acidic

(4) Five-year analysis of efficacy and safety of a bidirectional AAV gene therapy in Tay-Sachs sheepJ Clin InvestSeptember 30, 2025Toloo Taghian, Jillian Gallagher, Stephanie Bertrand, William C. Baker, Kalajan Lopez Mercado, Hector R. Benatti, Erin Hall, Yvette Lopez, Abigail McElroy, John T. McCarthy, et al.immunofluorescence included MAP2 (CH22103, Neuromics), recombinant anti-Olig2 antibody (ab109186, Abcam), MBP (NB600-717, Novus Biologicals), calcium-binding adapter molecule 1 (CP290, Biocare), and glial fibrillary acidic

(5) Laminar organization of the anterior olfactory nucleus—the interplay between neurogenesis timing and neuroblast migrationFrontiers in NeuroscienceApril 30, 2025Eduardo Martin-Lopez, Bowen Brennan, Marion Lefèvre, Natalie J. Spence, Kimberly Han, Charles A. Greer555,Thermo Fisher Scientific,"1:1,000" Myelin basic protein (MBP),Rat IgG2a clone 12,Novus Biologicals (nb600-717),AB_2139899,1:500,Goat α-rat IgG Alexa 488,Thermo Fisher Scientific,"1:1,000" NeuroD1,Mouse IgG2a,Abcam

(6) The X-Linked Intellectual Disability Gene, ZDHHC9 , Is Important for Oligodendrocyte Subtype Determination and MyelinationGliaMarch 19, 2025Rocio B. White, Angela R. Wild, Timothy P. O'Leary, Andrew J. Thompson, Stephane Flibotte, Angie Peng, Jason C. Rogalski, Mila Mair, Neeki Derhami, Shernaz X. Bamjiantibodies overnight at room temperature (MAG: 1:1000 dilution, 34–62,000, Invitrogen; MBP: 1:1000 dilution, NB600‐717, Novus bio; MOBP: 1:1000 dilution, #PA5‐100618, Invitrogen; CLDN11: 1:1000, 36–4500, Invitrogen; MOG:

(7) Anti-RGMa neutralizing antibody ameliorates vascular cognitive impairment in miceNeurotherapeuticsMarch 1, 2025Masaya Yamamoto, Takahide Itokazu, Hiroki Uno, Takakuni Maki, Nao Shibuya, Toshihide Yamashitaanti-Sox2 antibody (1:500; AB5603, Millipore), rat anti-myelin basic protein (MBP) antibody (1:200, NB600-717, NOVUS), rabbit anti-Cleaved caspase-3 antibody (1:1000; 9661, Cell signaling), and Alexa Fluor 647

(8) Inhibiting the Cholesterol Storage Enzyme ACAT1/SOAT1 in Myelin Debris-Treated Microglial Cell Lines Activates the Gene Expression of Cholesterol Efflux Transporter ABCA1BiomoleculesOctober 14, 2024Thao N. Huynh, Matthew C. Havrda, George J. Zanazzi, Catherine C. Y. Chang, Ta Yuan Changanti-ACAT1 (DM102) produced in the Chang lab, anti-PLIN2 (Proteintech 15294-1-AP), anti-MBP (Novus NB600-717) and anti-vinculin (Millipore 05-386) as a protein loading control. Blots were then washed and incubated

(9) Control of OPC proliferation and repopulation by the intellectual disability gene PAK1 under homeostatic and demyelinating conditionsbioRxivMay 4, 2024Yan Wang, Bokyung Kim, Shuaishuai Gong, Joohyun Park, Meina Zhu, Evelyn M. Wong, Audrey Y. Park, Jonathan Chernoff, Fuzheng GuoNOVUS), P-PDGFRα(Y754) (1:1000, #ab5460, Abcam), P-PDGFRα(Y849) (1:1000, #ab79318, Abcam), MBP (1:1000, #NB600–717, Novus), ERK1/2 (1:1000, #9102, Cell Signaling Technology), P-ERK1/2 (Thr202/Tyr204) (1:1000, #9101,

(10) Zfp488 promotes oligodendrocyte differentiation of neural progenitor cells in adult mice after demyelinationScientific reportsJanuary 1, 0001"Soundarapandian MM, Selvaraj V, Lo UG, Golub MS, Feldman DH, Pleasure DE, Deng W."

(11) Amyloid β1-42 oligomer inhibits myelin sheet formation in vitroNeurobiology of agingJanuary 1, 0001"Horiuchi M, Maezawa I, Itoh A, Wakayama K, Jin LW, Itoh T, Decarli C."

(12) Loss of osteoprotegerin expression in the inner ear causes degeneration of the cochlear nerve and sensorineural hearing lossNeurobiology of diseaseJanuary 1, 0001"Kao SY, Kempfle JS, Jensen JB, Perez-Fernandez D, Lysaght AC, Edge AS, Stankovic KM."

(13) The Importance of Inter-Species Variation in Traumatic Brain Injury-Induced Alterations of Microglial-Axonal InteractionsFrontiers in neurologyJanuary 1, 0001"Gorse KM, Lafrenaye AD."anti-Iba-1 (1:1,000; Cat.# 019-19741, Wako, Osaka, Japan), and rat anti-myelin basic protein (1:1,000; Cat.#NB600-717, Novus Biologicals, Littleton, CO, USA). For rat tissue, mouse anti-myelin MBP clone SMI-99 (1:1,000;

(14) IFN-gamma signaling in the central nervous system controls the course of experimental autoimmune encephalomyelitis independently of the localization and composition of inflammatory fociJournal of neuroinflammationJanuary 1, 0001"Lee E, Chanamara S, Pleasure D, Soulika AM."Goat anti-mouse IL4 R&D (Cat# AF-404-NA) MBP Rat anti-bovine myelin basic protein Novus biologicals (Cat# NB600-717) on a Nikon laser scanning confocal microscope, and images were tiled together using the Nikon NIS-Elements

(15) Oligodendroglial glycolytic stress triggers inflammasome activation and neuropathology in Alzheimer's diseaseScience advancesJanuary 1, 0001"Zhang X, Wang R, Hu D, Sun X, Fujioka H, Lundberg K, Chan ER, Wang Q, Xu R, Flanagan ME, et al."GSDMDC1 (catalog no. NBP2-33422, 1:500), AIF1/Iba1 (catalog no. NB100-1028, 1:1000), and MBP (catalog no. NB600717, 1:500) were purchased from Novus. Antibodies against phospho-Drp1 (Ser616, catalog no. 3455, 1:500),

(16) Quantitative proteomic analysis of the brainstem following lethal sarin exposureBrain researchJanuary 1, 0001"Meade ML, Hoffmann A, Makley MK, Snider TH, Schlager JJ, Gearhart JM."

(17) "Neuronal development in the cochlea of a nonhuman primate model, the common marmoset"Developmental neurobiologyJanuary 1, 0001"Hosoya M, Fujioka M, Murayama AY, Ozawa H, Okano H, Ogawa K."

(18) Krabbe disease successfully treated via monotherapy of intrathecal gene therapyThe Journal of clinical investigationJanuary 1, 0001"Bradbury AM, Bagel JH, Nguyen D, Lykken EA, Pesayco Salvador J, Jiang X, Swain GP, Assenmacher CA, Hendricks IJ, Miyadera K, et al."rabbit polyclonal anti-GALC (AB137750, Abcam; 1:200) and rat monoclonal anti–myelin basic protein (NB600717, Novus Biologics; 1:500) antibodies were diluted in antibody dilu- RESEARCH ARTICLE The Journal

(19) Microglia processes associate with diffusely injured axons following mild traumatic brain injury in the micro pigJournal of neuroinflammationJanuary 1, 0001"Lafrenaye AD, Todani M, Walker SA, Povlishock JT."Iba-1 (1:1000; Cat.# 019-19741, Wako, Osaka, Japan), and rat anti-myelin basic protein (1:1000; Cat#NB600-717, Novus Biologicals, Littleton, CO, USA). To reduce the amount of lipid within the tissue and enhance

(20) Combination HSCT and intravenous AAV-mediated gene therapy in a canine model proves pivotal for translation of Krabbe disease therapyMolecular therapy : the journal of the American Society of Gene TherapyJanuary 1, 0001"Bradbury AM, Bagel J, Swain G, Miyadera K, Pesayco JP, Assenmacher CA, Brisson B, Hendricks I, Wang XH, Herbst Z, et al."

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1/28/2020Excellent Myelin Basic Protein AntibodyDr. David SunnenA very sensitive myelin marker and generates robust fluorescence signals.

MBP Antibody (12)

MBP Antibody (12)

MBP Antibody (12)

MBP Antibody (12)

MBP Antibody (12) - BSA Free from Novus Biologicals, a Bio-Techne Brand

Specifications/Features

Questions and Answers

Novus Biologicals, a Bio-Techne Brand

Citations (57)

Reviews

MBP Antibody (12) - BSA Free from Novus Biologicals, a Bio-Techne Brand

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