Fig 1: siRNA knockdown of Nrf2 reverses the phenotypic transformation induced by ACT and FA in BV2 cells. A) Schematic diagram of plasmid transfection efficiency with GFP fluorescence in different time points (1/8/16/24 h), and qRT‐PCR was used to detect the knockdown efficiency of siRNA targeted Nrf2. B) The expression levels of IL‐1β, IL‐6, IL‐17A, and IL‐10 in BV2 cells were tested by ELISA. C)The levels of polarization biomarkers of CD86 and CD206 were detected by flow cytograms of BV2 cells in each group. D) Relative mRNA expression levels of M1 and M2 markers, and Nrf2/TREM2/DAP12 in the different groups. Results are presented as the mean ± SEM of three independent experiments. * p<0.05, ** p<0.01, and *** p<0.001, compared with the Si‐NC group; # p<0.05, ## p<0.01, and ### p<0.001, compared with the LPS‐induced cell model treated by ACT and FA.