Fig 1: Investigating the impact of KIT overexpression on PC-3 cell migration, invasion, and NK cell cytotoxicity. (A) Real-time qPCR validation of KIT overexpression in NK-92 cells. (B, C) Flow cytometry analysis of CD107a expression, a marker of NK cell degranulation, after co-culturing KIT-overexpressing NK-92 cells with PC-3 cells for 48 h. (D) Effect of KIT overexpression on the secretion of key NK cell cytotoxicity-related factors, including IFN-γ, Granzyme A (Gzms-A), Granzyme B (Gzms-B), and Perforin, after co-culturing KIT-overexpressing NK-92 cells with PC-3 cells for 48 h. (E, F) Apoptosis of PC-3 cells was assessed by flow cytometry after co-culture with KIT-overexpressing, vector control, or untreated NK-92 cells for 48 h. (G) Cell viability of PC-3 cells was evaluated by CCK-8 assay with KIT-overexpressing, vector control, or untreated NK-92 cells for 48 h. (H, I) The effects of KIT-overexpressing NK-92 cells on the migration and invasion of PC-3 cells were evaluated using Transwell assays. Quantification was based on the number of PC-3 cells that migrated or invaded through the membrane pores. *p-value < 0.05, **p-value < 0.01, ***p-value < 0.001, ****p-value < 0.0001.