Fig 2: TYMP inhibition partially rescues RIG-I activation induced phenotypes.A TYMP induction was measured using qRT-PCR. B HUVECs were treated with a control or RIG-I agonist in combination with Tipiracil. B Cell Death was assessed using a Caspase Glo assay (C) Annexin V luminescence assay and (D) Necrosis assay. E–F HUVECs were transfected with either a control siRNA or TYMP siRNA and treated with a control agonist or RIG-I agonist at the indicated doses. E mRNA levels showing efficient knockdown of TYMP 24 h post transfection. F Cell death was measured using Caspase-Glo assay. G–H Migration was assessed using a scratch assay in a 6-well TC plate. Scale bar = 100 uM. *P < 0.05, **P < 0.01, ***P < 0.005, ****P < 0.001 using ANOVA or two-tailed Student’s T-test. Bars represent mean ± SEM of independent replicates. One of two independent experiments.