Fig 1: Histologically damaged glomeruli have increased expression of C5aR1(A) C5AR1, the target of avacopan, is significantly elevated in damaged compared to unaffected Class III LN glomeruli. Each dot is expression from a 55μM spot. N=3 healthy control. N=3 Class III LN. N=3 Class V LN. N=1 C3G. N=1 PSGN. One way ANOVA with Tukey’s post-test for multiple comparisons. (B) Overlay of C5AR1 expression on UMAP from AMP Dataset (16). (C) Representative immunofluorescence (IF) co-staining of C5aR1 with cell-type specific markers CD68 (myeloid), PODXL (podocyte), CD31 (endothelial), aSMA (mesangial) in a glomerulus from a biopsy of a patient with Class III LN. (D) IF pixel-intensity based colocalization analysis of C5aR1 with CD68, PODXL, CD31, aSMA in Class III LN glomeruli and glomeruli from patients with non-SLE GN where C5aR1 was detectable at the protein level. Pearson correlation of 1 is perfect overlay. Dots are single glomeruli. Class III LN N=2 patients, C3G N=1 patient, PSGN N=1 patient.