Fig 2: SETDB1 regulated MMP3/10 expression in porcine SSCs. (A) qRT-PCR analysis of the relative mRNA level of MMP3/10 in porcine SSCs. (B) Western blot analysis of the protein levels of MMP3/10 and ACTB in porcine SSCs. The protein level of MMP3/10 relative to ACTB is shown in the column diagram. (C) Western blot analysis of the protein levels of H3K9me3 and histone H3 in porcine SSCs. The protein level of H3K9me3 relative to histone H3 is shown in the column diagram. Significance was presented as * p < 0.05, ** p < 0.01 or *** p < 0.001. Error bars represented the standard error of the mean (SEM).

Fig 3: MMP-2, MMP-10 and VEGF immunoexpression in canine melanoma. Immunohistochemistry results: (a) Strong VEGF immunoexpression in canine melanoma (white arrows), ×400. (b) Moderate MMP-2 immunoexpression in canine melanoma (black arrows), ×400. (c) Strong MMP-10 immunoexpression in canine melanoma (black arrows), ×400. (d) Negative control. No specific immunopositivity, ×400.

Fig 4: MK5 regulates matrix metalloproteinases’ expression in PC3 cells. (A–C) mRNA expression of several matrix metalloproteinases (MMPs): (A) MMP2, (B) MMP9, and (C) MMP10 in LNCaP and PC3 cells treated with different concentrations of GLPG. GAPDH mRNA was used as an internal control. (D) Immunoblots of the indicated proteins in LNCaP and PC3 cells treated with different concentrations of GLPG. GAPDH was used as a loading control. Images are the representation of three independent experiments (n = 3). (E,F) Quantifications of (E) MMP2, and (F) MMP9 level normalized to GAPDH in LNCaP and PC3 cells. (G) Immunoblots of the indicated proteins in PC3 cells upon MK5 depletion. GAPDH was used as a loading control. (H–J) Quantifications of (H) ERK3, (I) MMP2, and (J) MMP9 level normalized to GAPDH in PC3 cells upon MK5 depletion. VC = vehicle control (DMSO); shSCR = scrambled shRNA. One-way ANOVA, followed by Tukey’s post hoc analysis, was used for multiple group comparison: * = p < 0.05, ** = p < 0.005, and *** = p < 0.0005. Error bar represents standard error of the mean (SEM). The whole blots of Figure 5 could be found in Figure S2.