RNF20 Antibody

Product Specs
ItemRNF20 Antibody - BSA Free
CompanyNovus Biologicals, a Bio-Techne Brand
Price $459.00Supplier Page View Company Product Page
Catalog NumberNB100-2242
Quantity100 ul
ApplicationsChemotaxis,Immunoprecipitation,Western Blot,Chromatin Immunoprecipitation (ChIP)
Concentration1.0 mg/ml
Conjugate/TagUnconjugated
FormatTris-Citrate/Phosphate (pH 7.0 - 8.0)
PurityImmunogen affinity purified
ReactivityHuman,Mouse
TargetRNF20
HostRabbit
IsotypeIgG
Antibody TypePrimary Antibodies
ImmunogenThe immunogen recognized by this antibody maps to a region between residue 1 and 50 of human Ring Finger 20 using the numbering given in entry NP_062538.5 (GeneID 56254)
ClonalityPolyclonal
Research AreaChromatin Research, Zinc Finger
StorageStore at 4C. Do not freeze
NCBI Full Gene Namering finger protein 20
NCBI Gene AliasesBRE1A, BRE1, hBRE1

Specifications/Features

Available conjugates: Unconjugated

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Novus Biologicals, a Bio-Techne Brand

United States: 10771 E Easter Ave

Centennial, Colorado 80112

United States

Phone:
303-730-1950
888-506-6887

Email: novus@novusbio.com

Company Profile

Website: https://www.novusbio.com

(1) Hypoxia-inducible factor 1 recruits FACT and RNF20/40 to mediate histone ubiquitination and transcriptional activation of target genesCell reportsApril 23, 2024Lyu Y, Yang Y, Talwar V, Lu H, Chen C, Salman S, Wicks EE, Huang TY, Drehmer D, Wang Y, et al.

(2) CRISPR screen in regulatory T cells reveals modulators of Foxp3NatureJune 1, 2020Cortez JT, Montauti E, Shifrut E, Gatchalian J, Zhang Y, Shaked O, Xu Y, Roth TL, Simeonov DR, Zhang Y, et al.(Cell Signaling, Cat# 8204S) ub-Histone H2B Lys120 (Cell Signaling, Cat# 5546P) RNF20 Novus Biologicals, Cat# NB1002242 44 CHAPTER 3: METHODS 45 Mice for screen and RNP validation B6 Foxp3GFP-Cre mice41 were crossed

(3) Histone H2B monoubiquitination is a critical epigenetic switch for the regulation of autophagyNucleic acids researchFebruary 17, 2017Chen S, Jing Y, Kang X, Yang L, Wang DL, Zhang W, Zhang L, Chen P, Chang JF, Yang XM, et al.antibodies. Antibodies and western blot analysis The antibody against RNF20 was purchased from Novus (#NB100-2242). The anti-H2Bub1 antibody was purchased from Medimabs (#MM-0029). The anti-ubiquitin antibody was purchased

(4) Microarray Analysis Reveals Potential Biological Functions of Histone H2B MonoubiquitinationPloS oneJanuary 1, 2015Wu Y, Chen P, Jing Y, Wang C, Men YL, Zhan W, Wang Q, Gan Z, Huang J, Xie K, et al.purchased from Medimabs (#MM0029). Antibodies against RNF20 were purchased from Novus Biologicals (#NB100-2242). All HRP-conjugated secondary antibodies were purchased from Zhongshan Golden Bridge. Cells were lysed

(5) The epigenetic modifier ubiquitin-specific protease 22 (USP22) regulates embryonic stem cell differentiation via transcriptional repression of sex-determining region Y-box 2 (SOX2)The Journal of biological chemistryAugust 16, 2013Sussman RT, Stanek TJ, Esteso P, Gearhart JD, Knudsen KE, McMahon SB.

(6) Histone H2B ubiquitin ligase RNF20 is required for MLL-rearranged leukemiaProceedings of the National Academy of Sciences of the United States of AmericaMarch 5, 2013Wang E, Kawaoka S, Yu M, Shi J, Ni T, Yang W, Zhu J, Roeder RG, Vakoc CR.nitrocellulose for blotting. Antibodies. Anti–β-actin HRP (Sigma; A3854), anti-Rnf20 antibody (Novus Biological; NB100-2242), anti-H3K4me3 (Millipore; 07-473), anti-H3K79me2 (Abcam; ab3594), anti-H2Bub (Millipore; 05-1312),

(7) Promoters active in interphase are bookmarked during mitosis by ubiquitinationNucleic acids researchNovember 1, 2012Arora M, Zhang J, Heine GF, Ozer G, Liu HW, Huang K, Parvin JD.(Millipore -07-145), alexafluor647 tagged anti-Rabbit (Invitrogen—A21244), anti-RNF20 (Novus Biologicals—NB100-2242), anti-Lamin-B (Abcam—ab16048), anti-ubH2B (cell signaling technology) and anti-TFIIH p89 subunit (25). HeLa cells

(8) Histone H2B ubiquitin ligases RNF20 and RNF40 in androgen signaling and prostate cancer cell growthMolecular and cellular endocrinologyMarch 5, 2012Jääskeläinen T, Makkonen H, Visakorpi T, Kim J, Roeder RG, Palvimo JJ.

(9) The tumor suppressor CDC73 interacts with the ring finger proteins RNF20 and RNF40 and is required for the maintenance of histone 2B monoubiquitinationHuman molecular geneticsFebruary 1, 2012Hahn MA, Dickson KA, Jackson S, Clarkson A, Gill AJ, Marsh DJ.

(10) A member of the ETS family, EHF, and the ATPase RUVBL1 inhibit p53-mediated apoptosisEMBO reportsJuly 1, 2011Taniue K, Oda T, Hayashi T, Okuno M, Akiyama T.(Ab-1, CALBIOCHEM), BAX (6A7, BD Biosciences), Actin (A-2066, Sigma), ubH2B (NRO3, MEDIMABS), RNF20 (NB1002242, Novus Biologicals), PAF1 (A300-172A, Bethyl Laboratories) and H3K9Me3(sb8898, Abcam). RNA interference.

(11) Nucleosomal H2B ubiquitylation with purified factorsMethods (San Diego, Calif.)July 1, 2011Kim J, Roeder RG.

(12) Menin and RNF20 recruitment is associated with dynamic histone modifications that regulate signal transducer and activator of transcription 1 (STAT1)-activated transcription of the interferon regulatory factor 1 gene (IRF1)Epigenetics & chromatinSeptember 8, 2010Buro LJ, Chipumuro E, Henriksen MA.TX, USA, A300-105A), RNF20 (Abcam ab70495), RNF20-ChIP-grade (Novus Biologicals, Littleton, CO, USA, NB100-2242), FLAG (Sigma), Anti-rabbit or anti-mouse horseradish peroxidase (HRP) (Jackson Immunoresearch). Cell

(13) Histone ubiquitination associates with BRCA1-dependent DNA damage responseMolecular and cellular biologyFebruary 1, 2009Wu J, Huen MS, Lu LY, Ye L, Dou Y, Ljungman M, Chen J, Yu X.

(14) The histone H2B-specific ubiquitin ligase RNF20/hBRE1 acts as a putative tumor suppressor through selective regulation of gene expressionGenes & developmentOctober 1, 2008Shema E, Tirosh I, Aylon Y, Huang J, Ye C, Moskovits N, Raver-Shapira N, Minsky N, Pirngruber J, Tarcic G, et al.Millipore), anti-H3K4me3 (ab8580, Abcam), anti-PolII (ab5408, Abcam). Anti-RNF20 (Novus Biologicals, NB100-2242) and a noncommercial anti-RNF20 antibody (Y. Shiloh, unpubl.) were used for ChIP and Western blots,

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RNF20 Antibody

RNF20 Antibody

RNF20 Antibody

RNF20 Antibody - BSA Free from Novus Biologicals, a Bio-Techne Brand

Specifications/Features

Novus Biologicals, a Bio-Techne Brand

Citations (14)

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RNF20 Antibody - BSA Free from Novus Biologicals, a Bio-Techne Brand

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