LC3B Antibody - BSA Free NB100-2220 from Novus Biologicals, a Bio-Techne Brand

Product Specs
ItemLC3B Antibody - BSA Free
CompanyNovus Biologicals, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberNB100-2220
ImmunogenPolyclonal LC3B Antibody was made to a synthetic peptide made to an N-terminal portion of the human LC3B protein sequence (between residues 1-100). [UniProt# Q9GZQ8]
Concentration1.0 mg/ml
TargetLC3B
Conjugate/TagUnconjugated
FormatPBS
ReactivityAlligator,Avian,Avian - Chicken,Bacteria,Bovine,Canine,Chicken,Chinese Hamster,Golden Syrian Hamster,Guinea Pig,Hamster,Human,Insect - Drosophila melanogaster,Invertebrate,Monkey,Mouse,Porcine,Primate,Primate - Macaca mulatta (Rhesus Macaque),Rabbit,Rat,Zebrafish,Fish - Danio rerio (Zebrafish),Nematode - Caenorhabditis elegans
Quantity0.1 ml (also 0.025 ml)
HostRabbit
ClonalityPolyclonal
PurityImmunogen affinity purified
Research AreaAutophagy, Cancer, Cardiovascular Biology, Cellular Markers, Hypoxia, Innate Immunity, Lipid and Metabolism, Macroautophagy, Neuroscience
IsotypeIgG
ValidationKnockout Validated
Molecular Weight14.688 kDa
Antibody TypePrimary Antibodies
StorageStore at -20C
ApplicationsChemotaxis,IF/IHC,Flow Cytometry,ELISA,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunoblotting,Proximity Ligation Assay,Electron Microscopy,SDS-Page,Simple Western,Chromatin Immunoprecipitation (ChIP),Immunocytochemistry, Knockdown Validated,Block/Neutralize,Immunocytochemistry/ Immunofluorescence

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Sizes Available: 0.1 ml (also 0.025 ml)

Questions and Answers

Can this antibody be used for studying autophagy in cancer cells?

Yes, the LC3B antibody has been shown to be effective in studies investigating autophagy in cancer cells. For instance, it was utilized in the research by Ahn et al. (2016) to demonstrate how autophagy negatively regulates Notch1 signaling, which is crucial for tumor suppression.

What types of applications is this antibody suitable for?

The LC3B antibody has been employed in various applications, including immunoblotting, immunoprecipitation, and immunofluorescence staining, as evidenced by its use in studies such as those by Alotaibi et al. (2013) and Flower et al. (2020).

Is this antibody effective across different species?

Yes, the LC3B antibody is reactive with a wide range of species, including humans, mice, rats, zebrafish, and various other vertebrates and invertebrates, making it versatile for comparative studies in diverse biological contexts.

How has this antibody been validated in scientific research?

The LC3B antibody has been cited in numerous studies (over 1487 articles), indicating its widespread acceptance and validation in the scientific community for research related to autophagy and cellular processes. For example, it was used in the study by Han et al. (2022) to explore the degradation of Smurf1 in glioblastoma cells through autophagy.

Novus Biologicals, a Bio-Techne Brand

United States: 10771 E Easter Ave

Centennial, Colorado 80112

United States

Phone:
303-730-1950
888-506-6887

Email: novus@novusbio.com

Company Profile

Website: https://www.novusbio.com

(1) Chemically Derived Hepatic Progenitors Are Reprogrammed through Autophagy Suppression by A83-01International Journal of Stem CellsFebruary 28, 2028Hayoon Kim, Seunghee Kim, Soraya Salas-Silva, Dongho Choi, Ji Hyun Shinused for analysis: p62/SQSTM1 antibody (5114S, Cell Signaling Technology, 1：1,000), LC3B antibody (NB100-2220, Novus Biologicals, 1：10,000), and GAPDH antibody (ab128915, Abcam, 1：10,000). Horseradish peroxidase-conjugated

(2) KIF1A-mediated trafficking is required for neuronal autophagy in human neuronsbioRxivJuly 24, 2026Carris Borland, Jacob Popolow, Erika L. F. Holzbaurantibody,Abcam,"Cat# ab24170, RRID: AB_775978" polyclonal rabbit LC3 antibody,Novus Biologicals,"Cat# NB100-2220, RRID: AB_10003146" monoclonal rabbit Phospho-TFEB (Ser211) (E9S8N),Cell signaling Technology,Cat#

(3) Down-Regulation of TFEB With Defective Autophagy in the Susceptibility of Aging Kidneys to Septic Acute Kidney InjuryAging CellJuly 21, 2026Yu Xiang, Ying Fu, Zhiwen Liu, Yu Han, Wenwen Wu, Juan Cai, Dongshan Zhang, Zheng Dongtissues were blocked for 1 h with blocking buffer. Then the tissues were exposed to 1:5000 anti‐LC3 (NB100‐2220, Novus Biologicals) at 4°C overnight and incubated with poly‐HRP‐conjugated secondary antibody and goat

(4) PRMT5-Dependent Stabilization of VPS34 Orchestrates Copper Trafficking to Shield Cancer Cells from Cuproptosis and RadiotherapyAdv Sci (Weinh)July 8, 2026Wei Chen, Ming-Ying Xiao, Lan-Lan Guo, Hao-Yue Hu, Sui-Xian Zhang, Bai-Qiang Dong, Chen-Fei Wu, Xia-Hang Huang, Li Li, Run-Zhe Chen, et al.(sc‐28359) antibody was purchased from Santa Cruz Biotechnology (Dallas, TX, USA), and an anti‐LC3 (NB100‐2220) antibody was purchased from Novus Biologicals (Centennial, CO, USA). Antibodies against EEA1 (28347‐1‐AP),

(5) VPS35 mutation inhibits PINK1/parkin-mediated mitophagy via increased LRRK2 kinase activityBrainJuly 7, 2026Liselot Manders, Thibaut Heyninck, Dorien Imberechts, Bjørn Holst, Rejko Krüger, Wim Vandenbergheab53109), mouse anti-ATP5F1B (IF, 1:200; Abcam, ab14730), mouse anti-LC3B (WB, 1:2000; Novus Biologicals, NB100-2220), rabbit anti-PINK1 (WB, 1:1000; Novus Biologicals, BC100-494), mouse anti-mitofusin 2 (MFN2) (WB, 1:1000;

(6) The C-terminal region of TENT5 proteins drives ER-associated mRNA polyadenylation via FNDC3 interactionCell RepJune 23, 2026Lisa Viviani, Daniel Lacidogna, Sara Pennacchio, Maria Vittoria Mengozzi, Ugo Orfanelli, Leone Giordano, Tommaso Perini, Simone Cenci, Enrico MilanRRID:AB_2069033 rabbit anti-lambda light chains,Dako,Cat# A0194 rabbit anti-LC3B,Novus Biological,Cat# NB100-2220; RRID:AB_10003146 rabbit anti-LDHA,Cell Signaling Technology,Cat# 3582; RRID:AB_2066887 rabbit anti-PDI,"Dr.

(7) Biological Activity of Copper(II) and Palladium(II) Complexes with a Tetradentate S,O-Donor LigandInternational Journal of Molecular SciencesJune 23, 2026Anita Sarić, Marina Mitrović, Ana Barjaktarević, Snežana Jovanović Stević, Biljana Petrović, Žiko Milanović, Dušan Lj. Tomović, Andriana M. Bukonjić, Djordje Petrović, Mirjana Jakovljević, et al.Monoclonal Antibody (Biocompare, # M00300) and Rabbit Anti-LC3B Polyclonal Antibody (Novus Biologicals, # NB100-2220). The cells were then stained with secondary antibodies (1:200): anti-rabbit Alexa Fluor® 594 Conjugate

(8) Multi-omics reveal phosphatidic acid phosphatases modify Niemann-Pick type C disease severityiScienceJune 19, 2026Andrew B. Munkacsi, Gabriella E. Conway, Keri Multerer, Michael D. Jackson, Lauren Csaki, Tamayanthi Rajakumar, Jeffrey P. Sheridan, Eliatan Niktab, Wuh-Liang Hwu, Yin-Hsiu Chien, et al.REAGENT or RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, LC3,Novus,NB100-2220 GAPDH,GeneTex,GTX1001118 ,, "Chemicals, peptides, and recombinant proteins","Chemicals, peptides,

(9) Impact of BECLIN1 haploinsufficiency on goblet cell function and susceptibility to colitisCell Death & DiseaseJune 17, 2026Juliani Juliani, Sharon Tran, Tiffany J. Harris, Sarah L. Ellis, Aysha H. Al-Ani, Yashodha Wijebandara, Komal M. Patel, Samuel N. Young, Marco Evangelista, David Baloyan, et al.USA); p62/SQSTM1, 1:500 (5114, Cell Signalling Technology, Danvers, Massachusetts, USA); LC3B, 1:500 (NB100-2220, Novus Biologicals, Centennial Colorado, USA); β-Actin, 1:5000 (A2228, Sigma-Aldrich, St. Louis, Missouri,

(10) HIV Tat-Stimulated Microglial Extracellular Vesicles Are Enriched for Ferroptosis Mediators: Role of Dysregulated AutophagyJ Extracell BiolJune 11, 2026Seema Singh, Elias Horanieh, Craig L. Semerad, Palsamy Periyasamy, Susmita Sil, Shilpa BuchAnti‐β‐actin (Cat No. A5316) were obtained from Sigma‐Aldrich, St. Louis, MO, USA. Anti‐LC3B (Cat No. NB100‐2220), Anti‐lysosome‐associated membrane protein 2 (LAMP2; Cat No. NB300‐591), and Anti‐ferritin heavy chain

(11) Dietary Quercetin-3-Glucuronide Mitigates Oxidative Stress, Inflammation, and Fibroblast Transition by Regulating Nrf2 and Autophagy in Pulmonary FibrosisJournal of Agricultural and Food ChemistryJune 3, 2026Pei-Rong Yu, Chiao-Yun Tseng, Yu-Hsuan Liang, Yu-Ci Chang, Jing-Hsien Chen, Hui-Hsuan Linwere stained with primary antibodies Nrf2 (AF0639; Affinity Biosciences, Cincinnati, OH) and LC3-II (NB100–2220; Novus Biologicals, Centennial, CO); or α-SMA (NBP2–33006; Novus Biologicals, Centennial, CO) and vimentin

(12) Ruxolitinib reverses alopecia areata via a triple mechanism: JAK-STAT inhibition, localized oxidative stress attenuation and selective apoptosis modulationJournal of Translational AutoimmunityJune 1, 2026Meiling Zhu, Hao Mu, Bo Wang, Mengting Han, Zhao Ma, Xiaopan Nie, Tong Wang, Liyuan Zhang, Feng Liangfollowing primary antibodies: anti-LC3B (microtubule-associated protein 1 light chain 3 beta; NOVUS, NB100-2220), anti-cleaved caspase-3 (CST, 9662) (1:1000), and anti-GAPDH (glyceraldehyde-3-phosphate dehydrogenase;

(13) Huaier Polysaccharides Sensitize Anti-PD-L1 Therapy via Promoting Antitumor Immune Response in Triple-Negative Breast CancerInt J Biol SciMay 18, 2026Lin-Xi Zhou, Zi-Wei Wu, Yuan Tian, Ke-Fei Luo, Qin-Wen Pan, Yuan-Yin Xi, Peng Tang, Ling-Juan Zeng, Ling Zhong, Jun Jiang, et al.(AF7018) antibodies were from Affinity Biosciences (Cincinnati, OH, USA). Anti-PD-L1 (NBP1-43262), LC3 (NB100-2220), CD4 (NBP1-19371), CD8 (NB200-578), FoxP3 (NB100-39002) and Ki67 (NB110-89717) antibodies were from

(14) A human Staufen1 BAC transgenic mouse exhibits abnormal autophagy and neurodegeneration across the central nervous systemCell Death & DiseaseMay 14, 2026Stefan M. Pulst, Sharan Paul, Hieu Nguyen, Warunee Dansithong, Karla P. Figueroa, Mandi Gandelman, Nancy M. Bonini, Daniel R. Scolesantibody (Cell Signaling Technology, 5114); rabbit polyclonal anti-LC3B antibody (Novus Biologicals, NB100-2220); mouse monoclonal anti-TDP-43 (human specific) antibody [(1:7000), Proteintech Group, Inc., (Cat #60019-2-Ig)];

(15) RPS6KA3/RSK2-mediated phosphorylation of DRAM2 promotes lysosomal targeting and autophagic flux in melanomaAutophagyMay 3, 2026Ga-Eun Lee, Soo-Bin Nam, Eunyoung Moon, Yang Hoon Huh, Hye Sun Park, Seungjin Na, Jin Young Kim, Eun Hee Han, Hana Cho, Jung Hoon Choi, et al.648,002), anti-CD63 (Invitrogen, 10628D), anti-CANX (Cell Signaling Technology, 2433), anti-LC3B (Novus, NB100-2220), anti-SQSTM1 (Cell Signaling Technology, 39,749), anti-VCL (Santa Cruz Biotechnology, sc-25336), anti-GST

(16) Antitumor Activity of Sodium Selenite, Palbociclib, and Disulfiram Against Osteosarcoma and Rhabdomyosarcoma Cell LinesDrug Dev ResMay 3, 2026María Ángeles Chico, Kevin Doello, Raul Ortiz, Mercedes Peña, Consolación Melguizo, Cristina Mesas, Jose PradosAbcam), rabbit anti‐PARP1 antibody (1:1000, ab32138, Abcam) and rabbit anti‐LC3β antibody (1:1000, NB100‐2220, Novus Biologicals). Then, they were incubated for 1 h at room temperature with anti‐mouse IgGκ BP‐HRP

(17) Mitochondrial protein import stress causes lysosomal damage and progressive tissue atrophyEMBO ReportsApril 27, 2026Nicholas A Brennan, Xiaowen Wang, Arnav Rana, Sanaea Z Bhagwagar, Jason Horton, Patricia M Kane, Xin Jie Chen(1:1000; #2535, CST); rabbit polyclonal AMPKα (1:1000; #2532, CST); rabbit polyclonal LC3B (1:1000; #NB100-2220, Novus Biologicals); rat monoclonal LAMP2 (1:100; #ABL-93, DSHB); rabbit polyclonal GAA (1:1000; #PA5-117464,

(18) Targeting VPS4 elicits STING-driven anti-tumor immunity to suppress rhabdomyosarcoma growthOncogeneApril 24, 2026Ray Zhang, Longgui Chen, Xinwen Liang, Jiawen Zhang, Kouta Hamamoto, Tatsuya Hattori, Venugopal Vangala, Todd D. Schell, Giselle Saulnier Sholler, Yoshinori Takahashi, et al.ATF-3 (Cell Signaling Technology #18665S, 1:1000), and LC3B (Novus Biologicals [Centennial, CO, USA] #NB100-2220, 1:5000). The following inhibitors were used to dissect innate immune signaling pathways: BMS-345541

(19) Autophagy selectively clears ER in TNF-α-induced muscle atrophyAutophagy RepApril 17, 2026Ursula K. Dueren, Alan An Jung Wei, A. Elisabeth Gressler, Simon Rapp, Oliver Popp, Robert Kerridge, Viviana Buonomo, Paolo Grumati, Philipp Mertins, Matthias Selbach, et al.monoclonal anti-LAMP1-FITC (BioLegend, sc-121606 1:1000), rabbit polyclonal anti-LC3B (Novus Biologicals, NB100-2220 1:500), mouse monoclonal anti-MyHC (Sigma-Aldrich, M4276 1:1000), rabbit polyclonal anti-p62 (Invitrogen,

(20) Ghrelin Receptor Deletion or Pharmacological Inhibition Improves Muscle Function in Aging Male MiceAging CellApril 15, 2026Haiming L. Kerr, Kora Krumm, Nornubari Myree, Artur Rybachok, Elizabeth Dacek, Brynn Irwin, Siyi Jiang, Lucas Caeiro, Barbara Anderson, Theresa Li, et al.antibodies: anti‐SQSTM1/p62 (1:1000, Ab56416, Abcam), anti‐PGC1 alpha (1:1000, ab191838, Abcam), LC3B (1:500, NB100‐2220 Novus), total OXPHOS Rodent WB Antibody Cocktail (1:1000, ab110413, Abcam), anti‐Parkin antibody (1:500,

Write a Review

(1)

5/10/2019Excellent Antibody for LC3B Detection by Immunoblotting from Novus Elena SeranovaWould definitely recommend this antibody for western blotting. So far it has worked in all of the cell lines we tried it on in our lab.
(1)

4/20/2017LC3B Antibody - Works Well KAVITHA SUBRAMANIANLC3B - I and II was probed by Western blotting on murine primary bone marrow derived macrophage lysates. Antibody detects two bands of LC3B I and II around ~17 kDa.