Fig 2: LRRFIP1 and FLII interact with human NLRP1 inflammasome A–F Interaction of NLRP1 with LRRFIP1 and FLII in K562 cells (A, D–F), HEK293 cells (B) and M‐PBMCs (C) assayed by co‐immunoprecipitation assays and PLA (D–F). Endogenous proteins (A, C–F) and transfected FLAG‐tagged full‐length NLRP1, NLRP1ΔCARDΔPYD, NLRP1_S1213A and UPA‐CARD (B) were analyzed. The number of interactions per cell (E) and the percentage of cytosolic interactions (F) are shown (n = 100 cells). Data are shown as the means ± SEM. P values were calculated using one‐way ANOVA and Tukey's multiple range test. n.s., non‐significant; ****P < 0.0001. #, non‐specific band. Source data are available online for this figure.

Fig 3: Lrrfip1 regulates hematopoiesis through the Nlrp1 inflammasome in zebrafish A–H Number of erythrocytes (A), neutrophils (B, F, H), macrophages (C), and HSPCs (D), and caspase‐1 activity (E) in nlrp1 crispant larvae of 2 dpf obtained by injecting one‐cell stage embryos with standard, nlrp1, lrrfip1a and/or lrrfip1b crRNAs/Cas9 complexes. Note that either lrrfip1a or lrrfip1b crRNAs/Cas9 complexes target both lrrfip1 paralogs (see Appendix Fig S10). (G, H) Larvae were also treated by bath immersion with 100 μM of the caspase‐1 inhibitor VX‐765. Representative images of neutrophils in lrrfip1 crispant larvae using Tg(lyz:dsRED) reporter line are shown in (G). Each dot represents one individual and the means ± SEM for each group are also shown. P values were calculated by Student's t‐test. n.s., non‐significant; *P<0.05; **P<0.01; ****P<0.0001. Source data are available online for this figure.