GLI-2 Antibody AF3526 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemGLI-2 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $489.00Supplier Page View Company Product Page
Catalog NumberAF3526
ApplicationsBioassay,Immunohistochemistry,Western Blot,Proximity Ligation Assay,Chromatin Immunoprecipitation (ChIP),Chromatin Immunoprecipitation Sequencing
ReactivityHuman,Mouse
Quantity100 ug (also 25 ug)
IsotypeIgG
TargetGLI-2
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
HostSheep
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant human GLI-2, Ala2-Glu91, Accession # BAA25668
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Full Gene NameGLI family zinc finger 2
NCBI Gene AliasesCJS, HPE9, PHS2, THP1, THP2

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human GLI-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant mouse GLI-2 and 5% cross-reactivity with recombinant human (rh) GLI-1 and rhGLI-3 is observed.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Loss-of-function variants in ciliary genes confer high risk for tetralogy of FallotSci AdvOctober 10, 2025Yan Zhou, Tao Jiang, Jimiao Gao, Jie Zang, Xuming Mo, Shen Yue, Yiqiang Cui, Qiuye Wang, Min Da, Jing Xu, et al.primary antibodies against GLI1 and GLI2 (GLI1: R&D Systems, AF3324, RRID:AB_663902; GLI2: R&D Systems, AF3526, RRID:AB_2279108) for 2 hours, followed by incubation with a secondary antibody for 1 hour at room temperature.

(2) A splicing variant in EFCAB7 hinders ciliary transport and disrupts cardiac developmentJournal of Biological ChemistryMarch 1, 2025Xin Yang, Qiuye Wang, Tianyuan Li, Yan Zhou, Jimiao Gao, Wanting Ma, Na Zhao, Xinyue Liu, Zihe Ai, Steven Y. Cheng, et al.incubated with anti-GLI1 and anti-GLI2 primary antibody (GLI: R&D Systems, AF3324; GLI2: R&D Systems, AF3526) for 2 h, and then for 1 h with secondary antibody at room temperature. Hyperactive pG-Tn5 transposonase

(3) The O-glycosyltransferase C1GALT1 promotes EWSR1::FLI1 expression and is a therapeutic target for Ewing sarcomaNature communicationsFebruary 2, 2025Banday S, Mishra AK, Rashid R, Ye T, Ali A, Li J, Yustein JT, Kelliher MA, Zhu LJ, Deibler SK, et al.with 5 µg anti-GLI1 antibody (R&D Systems, Cat#: AF3324) or anti-GLI2 antibody (R&D Systems, Cat#: AF3526) overnight at 4 °C with constant rotation. In the ChIP experiment shown in Fig. 3i, normal goat IgG

(4) A Druggable UHRF1/DNMT1/GLI Complex Regulates Sonic Hedgehog-Dependent Tumor GrowthMolecular cancer research : MCRNovember 3, 2022Yang F, Rodriguez-Blanco J, Long J, Swiderska-Syn M, Wynn DT, Li B, Shen C, Nayak A, Ban Y, Sun X, et al.antibodies were used for immunoprecipitation: GLI1 (prepared as described before [39]), GLI2 (Cat# AF3526, R&D Systems, RRID:AB_2279108), rabbit IgG (Cat# 12370, MilliporeSigma), sheep IgG (Cat# 5-001-A, R&D

(5) RNA-seq and ChIP-seq Identification of Unique and Overlapping Targets of GLI Transcription Factors in Melanoma Cell LinesCancersSeptember 19, 2022Kurtović M, Piteša N, Bartoniček N, Ozretić P, Musani V, Čonkaš J, Petrić T, King C, Sabol M.antibodies against GLI1 10 μg/sample (AF3324, R&D Systems, Minneapolis, MN, USA) [24], GLI2 4 μg/sample (AF3526, R&D Systems) [24], and GLI3 10 μg/sample (AF3690, R&D Systems) overnight. Library preparation for next-generation sequencing

(6) Regulation of GLI1 by cis DNA elements and epigenetic marksDNA repairJuly 1, 2019Taylor R, Long J, Yoon JW, Childs R, Sylvestersen KB, Nielsen ML, Leong KF, Iannaccone S, Walterhouse DO, Robbins DJ, et al.

(7) Distinct immunocyte-promoting and adipocyte-generating stromal components coordinate adipose tissue immune and metabolic tenorsScience immunologyMay 3, 2019Spallanzani RG, Zemmour D, Xiao T, Jayewickreme T, Li C, Bryce PJ, Benoist C, Mathis D.ProLong Diamond Antifade Mountant (Thermofisher). Primary Abs were goat anti-mouse IL-33 (R&D Systems, #AF3526, 1:50 dilution), APC-conjugated Syrian Hamster anti-mouse PDPN (Biolegend, #AF3526, 1:100), or rabbit

(8) LncRNA wires up Hippo and Hedgehog signaling to reprogramme glucose metabolismThe EMBO journalNovember 15, 2017Zheng X, Han H, Liu GP, Ma YX, Pan RL, Sang LJ, Li RH, Yang LJ, Marks JR, Wang W, et al.Santa Cruz Biotechnology. The following antibodies were used for ChIP or RNA immunoprecipitation (RIP): GLI2 (AF3526) from R&D Systems; p300 (61402), H3K27Ac (39133) from Active Motif. RNAScope® assay, immunohistochemistry

(9) SOX2 regulates self-renewal and tumorigenicity of human melanoma-initiating cellsOncogeneSeptember 18, 2014Santini R, Pietrobono S, Pandolfi S, Montagnani V, D'Amico M, Penachioni JY, Vinci MC, Borgognoni L, Stecca B.

(10) GLI2 transcription factor mediates cytokine cross-talk in the tumor microenvironmentThe Journal of biological chemistryJune 17, 2011Elsawa SF, Almada LL, Ziesmer SC, Novak AJ, Witzig TE, Ansell SM, Fernandez-Zapico ME.

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GLI-2 Antibody

GLI-2 Antibody

GLI-2 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (10)

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GLI-2 Antibody from R&D Systems, a Bio-Techne Brand

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