Phospho-Src Family (Tyr416) (D49G4) Rabbit mAb 6943S from Cell Signaling Technology

Product Specs
ItemPhospho-Src Family (Tyr416) (D49G4) Rabbit mAb
CompanyCell Signaling Technology
Price $401.00 Inquire
Catalog Number6943S
Quantity100 µl
ApplicationsW, W-S, IP
ReactivityH, M, R, Mk, Dg
ModificationsFyn (Tyr420) phosphate/Hck (Tyr411) phosphate/Lck (Tyr394) phosphate/Lyn (Tyr397) phosphate/Src (Tyr419) phosphate/Yes (Tyr426) phosphate
HostRabbit
ClonalityMonoclonal
TargetYes (Tyr426) phosphate;Lyn (Tyr397) phosphate;Lck (Tyr394) phosphate;Hck (Tyr411) phosphate;Src (Tyr419) phosphate;Fyn (Tyr420) phosphate
NCBI Full Gene NameLYN proto-oncogene, Src family tyrosine kinase, YES proto-oncogene 1, Src family tyrosine kinase, LCK proto-oncogene, Src family tyrosine kinase, SRC proto-oncogene, non-receptor tyrosine kinase, HCK proto-oncogene, Src family tyrosine kinase, FYN proto-oncogene, Src family tyrosine kinase
NCBI Gene AliasesJTK9, JTK8, p61Hck, IMD22, p59Hck, p56lck, ASV, SAIDV, AIPCV, LSK, YT16, HsT441, SLK, c-yes, Yes, p59-FYN, c-SRC, p56Lyn, SYN, THC6, p60-Src, SRC1, pp58lck, P61-YES, p53Lyn
Research AreaMAP Kinase Signaling
IsotypeIgG
CloneD49G4
Molecular Weight60

Questions and Answers

What applications has the Phospho-Src Family (Tyr416) antibody been used for in research studies?

The Phospho-Src Family (Tyr416) antibody has been shown to work in applications such as Western blotting and immunocytochemistry, where it is utilized to detect phosphorylated Src family kinases, which are critical in various signaling pathways related to cancer and cell migration.

Can this antibody be used to study the role of Src family kinases in cancer progression?

Yes, the Phospho-Src Family (Tyr416) antibody is relevant for studying Src family kinases in cancer progression, as it has been cited in numerous studies that investigate their role in tumorigenesis and metastasis, particularly in the context of signaling pathways that regulate cell motility and invasion.

Is there any evidence of this antibody being used in vivo for cancer research?

While specific in vivo applications are not detailed in the provided information, the antibody's role in detecting phosphorylated Src family kinases suggests its potential utility in vivo, particularly in studies examining tumor growth and metastasis in animal models, as indicated by its citation in a substantial number of scientific articles.

How does the specificity of this antibody contribute to its effectiveness in research?

The Phospho-Src Family (Tyr416) antibody is designed to specifically recognize the phosphorylated form of Src family kinases at Tyr416, which is crucial for their activation. This specificity allows researchers to accurately assess the activation state of these kinases in various cellular contexts, enhancing the reliability of experimental results related to signaling pathways in cancer and other diseases.

Cell Signaling Technology

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Danvers, Massachusetts 01923

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Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) PTPRE promotes gastric cancer cell resistance to 5-fluorouracil by inhibiting ferroptosis via the Src/FAK/TRIB3 axisPLoS OneJune 18, 2026Ming Liu, Xiaomei Liao, Fangchao Wang, Pengqing Jiao, Zhongkai Wang1:1000 dilution), rabbit anti-p-FAK (Cat. No. 8556S, 1:1000 dilution), and rabbit anti-p-Src (Cat. No. 6943S, 1:1000 dilution; Cell Signalling Technology, Danvers, MA, USA); Mouse anti-TRIB3 (Cat. No. 43043S,

(2) A MUC1-ALIX complex regulates extracellular vesicle cargo loading with activated SRC to enhance pancreatic cancer progressionCancer LettersMay 28, 2026Ying Huang, Kristine V. Hoagstrom, Clara S. Mundry, Tuo Hu, Heather C. Jensen-Smith, Jacob Wragge, Phong Le, Amina Baniya, Kyle L. McAndrews, Thomas C. Caffrey, et al.(Tyr527),Cell Signaling Technology,Cat#: 2105 Phospho-Src Family (Tyr416),Cell Signaling Technology,Cat#: 6943 Rab7 (Host: rabbit),Cell Signaling Technology,Cat#: 9367 Src (Host: rabbit),Cell Signaling Technology,Cat#:

(3) NCKAP1 coordinates focal adhesion and adherens junction dynamics in endothelial barrier maintenance and angiogenesisiScienceMay 15, 2026Yunyun Xiao, Paul-Lennard Mendez, Christian Hiepen, Jerome Jatzlau, Petra KnausRRID:AB_2160882 Rabbit Monoclonal anti-Phospho-Src Family (Tyr416) (D49G4); WB 1:1000,Cell Signaling Technology,Cat#6943; RRID:AB_10013641 Rabbit Monoclonal anti-Phospho-FAK (Tyr397) (D20B1); WB 1:1000,Cell Signaling Technology,Cat#8556;

(4) Dual Role of Cancer Epithelial-Specific TRAF3 in Regulating Breast Cancer Cell Survival and Lymphocyte ActivityInt J Mol SciMay 15, 2026Chaido Sirinian, Anne-Lise de Lastic, Harry Zaverdas, Martha Nifora, Dimitra Georgakopoulou, Martina Samiotaki, Maria Ioanna Argentou, Stavros Peroukidis, Søren E. Degn, Maria Rusan, et al.in this study were the following: Phospho-AKT (#4060 1:2000, CST, Danvers, MA, USA), phospho-src (#6943, 1:1000, CST), phospho-EGFR (AF3394, 1:200, R&D systems, Minneapolis, MN, USA), Vimentin (#5741, 1:1000

(5) CD1d functions as a ligand for PIRA2 to drive macrophage activation in nonalcoholic fatty liver diseaseCell Death & DiseaseApril 27, 2026Xiaosheng Tan, Qingwen Li, Zhibo Ma, Lingjuan Sun, Xiangli Zhao, Jianlin Chen, Jingzeng Wang, Xiufang Weng, Li Chen, Zhishui Chen, et al.Mouse mAb,Servicebio,cat#: GB14023; Phospho-Src Family (Tyr416) Rabbit mAb (clone D49G4),CST,cat#: 6943 T; RRID: AB_10013641 Src Rabbit mAb (clone 36D10),CST,cat#: 2109S; RRID: AB_2106059 NF-κB p65 Mouse

(6) Gemcitabine-induced β4 integrin drives cancer progression and gemcitabine resistance in pancreatic cancerJournal of Experimental & Clinical Cancer ResearchApril 24, 2026Yoshinobu Kariya, Haruto Suzuki, Yukiko Kariya, Michiru Nishita1:10,000), phospho-Src (Tyr416) (#2101, Cell Signaling Technology, 1:1,000), phospho-Src (Tyr416) (D49G4, #6943, Cell Signaling Technology, 1:1,000), phospho-Src (Tyr530) (#2105, Cell Signaling Technology, 1:1,000),

(7) Sulfation of Chondroitin Sulfate Regulates Neuronal Morphology via Src-Family Signaling with Likely Contribution from FynCellsApril 22, 2026Saya Kubosaka, Tadahisa Mikami, Hiroshi Kitagawapurchased from BioLegend (San Diego, CA, USA). Anti-phospho-Src family (tyrosine 416 [Tyr416]) (D49G4, #6943) and anti-Fyn (#4023) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Alexa

(8) Cardiomyocyte Cyclin-dependent kinase 9 directly binds to and phosphorylates NF-κB p65 subunit to drive cardiac inflammation and remodelingNature CommunicationsMarch 24, 2026Shiju Ye, Yanbo Zhao, Hanxiao Tu, Xue Han, Tong Liu, Yingchao Gong, Jiangting Lu, Tingting Jin, Wu Luo, Xuefeng Qu, et al.(#3033), IκB kinase-beta (IKKβ) (#8943), Phospho-IKKα/β (Ser176/180) (#2697), Phospho-c-Src (Tyr416) (#6943), Phospho-PKA C (Thr197) (#5661), HEXIM1(#12604 T), PKC-alpha (#2056), Phospho-PKC alpha (Thr638) (#9375)

(9) Sphingosine‑1‑phosphate receptor 1 enhances olfactory receptor 51E1‑mediated inhibition of proliferation via Src/JNK signaling in prostate cancer cellsOncol RepMarch 19, 2026Kiheon Kim, Jinwoo Lee, Chulwon Choi, Jungnam Bae, Hee-Jung Choi, Won-Ki Huhanti-phospho-ERK1/2 (T202/Y204) (cat. no. 4370), anti-SRC (cat. no. 2109), anti-phospho-SRC (Tyr416; cat. no. 6943), anti-JNK (cat. no. 9252), anti-phospho-JNK (T183/Y185; cat. no. 4668), anti-p38 MAPK (cat. no. 8690)

(10) PTEN Loss Promotes PI3Kβ Phosphorylation and EPHA2/SRC/p-PI3KβY962 Complex Assembly to Drive TumorigenesisCancer DiscoveryMarch 2, 2026Shuang Tang, Qian Zhou, Johann S. Bergholz, Tao Jiang, Weihua Wang, Renlei Ji, Wendi Huang, Jiachen Wu, Yutong Li, Xiaochun Wan, et al.(Cell Signaling Technology, cat. #2109, RRID: AB_2106059), p-SRCY416 (Cell Signaling Technology, cat. #6943, RRID: AB_10013641), GST (Cell Signaling Technology, cat. #2622, RRID: AB_331670), c-Myc (Abcam, cat.

(11) Role of lipid rafts in the FGFR2c-mediated oncogenic signaling by involvement of TRPA1 channel in pancreatic ductal adenocarcinoma cellsCell Death & DiseaseFebruary 24, 2026Vanessa Mancini, Valeria Manganelli, Tina Garofalo, Maurizio Sorice, Salvatore Raffa, Dafne Scullari, Danilo Ranieri, Francesca Belleudianti-phospho-Sarcoma kinase (Src) Family (Tyr416, D49G4) (Cell Signaling Technology, Beverly, MA; USA;6943), monoclonal antibodies anti-p-MTOR (Ser 2448; Cell Signaling; Beverly, MA, USA; 5536S), anti-p-p44/42

(12) Pan-cancer N-glycoproteomic atlas of patient-derived xenografts uncovers FAT2 as an actionable surface targetCell Reports MedicineFebruary 17, 2026Meinusha Govindarajan, Salvador Mejia-Guerrero, Shawn C. Chafe, Shahbaz Khan, Wei Shi, Matthew Waas, Martin A. Rossotti, Amanda Khoo, Lydia Y. Liu, Vladimir Ignatchenko, et al.RRID:AB_2106047 Phospho-Src Family (Tyr416) (D49G4) Rabbit monoclonal antibody,Cell Signaling Technology,Cat# 6943; RRID:AB_10013641 EGFR (D38B1) Rabbit monoclonal antibody,Cell Signaling Technology,Cat# 4267; RRID:AB_2246311 Anti-Human EGFR

(13) An Extracellular Matrix–Producing Subset of Cancer-Associated Fibroblasts Drives Chemoresistance in Breast Cancer via SRC Activation and G0S2 UpregulationCancer ResearchFebruary 16, 2026Isabella Hofer, Yann Kieffer, Arianna Mencattini, Hugo Croizer, Rana Mhaidly, Stéphanie Descroix, Christophe Le Tourneau, Maud Kamal, Constance Lamy, Claire Bonneau, et al.(Cell Signaling Technology, #999S) or 1:2,000 phospho-SRC Tyr416 (Cell Signaling Technology, cat. #6943, RRID: AB_10013641) in TBST supplemented with 5% BSA (EUROMEDEX, # 04-100-812-C). On the next day, membranes

(14) Nbeal2 Inactivation Triggers Abl1 Stabilisation and Dysregulated Subcellular Localisation of the Multi‐Drug‐Resistant Protein MDR1 (ABCB1) in Mast CellsImmunologyFebruary 1, 2026Raphaela Marquardt, Nico Andreas, Ronja Herrnberger, Claudia Küchler, Katrin Hornung, Bernhard Nieswandt, Marco Groth, Paul M. Jordan, Oliver Werz, Julia Drube, et al.(#9251), JNK1/2 (#9262), ABCB1 (#13978), tubulin (#3873), p‐tyrosine (Y) (#9411), pan‐pY‐416 SFK (#6943), Lyn (#2732), c‐Kit (#3074), Fyn (#4023), pAbl1 (#2861), Abl1 (#2862) (all from Cell Signalling Technology),

(15) SIRT2-mediated deacetylation of LCK governs the magnitude of T cell receptor signalingNature ImmunologyJanuary 29, 2026Imene Hamaidi, Pingyan Cheng, Soo Young Jun, Alak Manna, Min-Hsuan Wang, Anh Nguyen, Ismail Can, Min G. Zhang, Odesha O. Taylor, Luis Uriel Lopez Bailon, et al.dilution), Phospho-ZAP70 (Tyr319)/Syk (Tyr352) (2717, 1:1,000 dilution), Phospho-Src Family (Tyr416) (6943, 1:1,000 dilution), phospho-LCK (Tyr505) (37458, 1:1,000 dilution) and Phospho-p44/42 MAPK (Erk1/2)

(16) Human organoid tumor transplantation identifies functional glioblastoma-microenvironment communication mediated by PTPRZ1Cell ReportsJanuary 27, 2026Weihong Ge, Ryan L. Kan, Can Yilgor, Elisa Fazzari, Patricia R. Nano, Daria J. Azizad, Heer Shinglot, Matthew Li, Joyce Y. Ito, Christopher Tse, et al.Sigma-Aldrich A1978), anti-SRC (1:10000, Abcam ab109381), anti-phospho-SRC (1:1000, Cell Signaling Tech 6943T). The membranes were incubated overnight at 4°C. Secondary antibodies targeting mouse (1:10000, Fisher

(17) Formononetin ameliorates SP-induced urticaria in mice via suppressing TAK1/MAK signaling pathwayPLOS OneJanuary 23, 2026Yan Wu, Chunyu Liantibodies (Cell Signaling Technology) incubated at 4˚C overnight: anti-GAPDH (#2118,1:2000), anti-p-TAK1 (#6943, 1:1000), anti-TAK1 (#4023, 1:1000), anti-p-p38 (#2731, 1:1000), anti-p38 (#2796, 1:1000), anti-p-Erk1/2

(18) RSPO4 exerts tumor suppression through antagonizing canonical and non-canonical Wnt signalingInternational Journal of Biological SciencesJanuary 1, 2026Zhenfang Du, Lili Li, Xingsheng Shu, Chen Li, Qian Taop44/42 MAPK (Erk1/2) (#9102), RhoA (67B9) (#2117), Src (#2108), phospho-Src Family (Tyr416) (D49G4) (#6943), phospho-c-Jun (Ser63) II (#9261) and E-cadherin (#3195) (Cell Signaling, Beverly, MA); phospho-RhoA

(19) The Crosstalk Between CRL5 and APC/C E3 Ligases Regulates Metastasis and Chemosensitivity of Cancer CellsAdvanced ScienceJanuary 1, 2026Danrui Cui, Ruirui Qu, Tianqi Li, Linchen Wang, Xiaoyu Chen, Shengpeng Shao, Xue Liang, Jun Xu, Yi Sun, Xiufang Xiong, et al.ab183748), integrin β1 (Cell Signaling Technology, 34 971), p‐SRC (Y416) (Cell Signaling Technology, 6943), ATM (Cell Signaling Technology, 2873), Cyclin B1 (Cell Signaling Technology, 4138), RPB1 (Cell Signaling Technology

(20) Mechanotransduction-driven macrophage polarization via Integrin-SRC-STAT6 pathway in distraction osteogenesisJournal of Orthopaedic TranslationDecember 24, 2025Xu Yan, Haixing Wang, Xuan Lu, Linlong Li, Shanshan Bai, Zitong Li, Han Su, Rongjie Wu, Hui Chen, Yinuo Fan, et al.Cell Signaling Technology, Danvers, USA), SRC (1:1000, #2109, Cell Signaling Technology, Danvers, USA), p-SRC (1:1000, #6943, Cell Signaling Technology, Danvers, USA), Arg-1 (1:500, PTM-5730, PTMBio, Hangzhou, China), GAPDH (1:5000,

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12/18/2015Great Phospho-Src Family Antibody A MOur lab works to elucidate the impact of kinases in cancer progression, and our research often includes Western Blots in order to determine kinase protein and activity levels in various cancer cell ... Read Review

Phospho-Src Family (Tyr416) (D49G4) Rabbit mAb

Phospho-Src Family (Tyr416) (D49G4) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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Phospho-Src Family (Tyr416) (D49G4) Rabbit mAb from Cell Signaling Technology

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