Fig 1: Single-cell RNA-sequencing analysis of the cell population in OA.A Gene expression plots of CHI3L1, AEBP1, STEAP1 and PLIN2. Dot colour corresponds to the level of gene expression in each cell. t-SNE, t-distributed stochastic neighbour embedding. B Representative immunohistochemistry staining of AEBP1, CHI3L1 and STEAP1 in OA and healthy cartilage tissues. Scale bar, left, 500 µm; right, 50 µm, and comparative quantification of positive cells of different areas (superficial, middle, deep) between OA and healthy cartilage tissues displayed by box plot (n = 5). *p < 0.05.