Fig 2: The JAK2-STAT pathway serves as the major downstream signaling pathway of the mutated PDGFB. mIF demonstrate the important downstream signaling pathway markers of PDGFRβ (p-JAK, p-Src, p-Erk1/2 and p-PLCγ) in FIA sections and NCA sections (A). Scale bar, 100 μm. Statistical analysis is performed to compare the mean fluorescence intensity of different signal markers between NCAs (n = 4) and FIAs (n = 5) (C). The expression of p-STAT1 and p-STAT3, which are downstream of p-JAK2, is detected by mIF in FIAs and NCAs. Scale bar, 50 μm (B). Statistical analysis of mean fluorescence intensity of these marks between NCAs (n = 4) and FIAs (n = 5) (D). Western blotting displays the expression of the important downstream signal pathway markers of PDGFRβ (p-JAK2, p-Src, p-Erk1/2 and p-PLCγ) in the HBVSMCs underwent different treatment in vitro (E). Relative density (normalized to Control) of immunoblot bands from (E) corresponding to pPLCγ, p-JAK2, p-Src, and p-ERK1/2 (n = 3) (F). 'n' represented the number of samples. Three random fields were selected for statistical analysis in each sample, and the average value represented the detection value of this marker in this sample. Tukey's multiple comparisons test is conducted to evaluate the statistical differences. ns, no significant; ***padj < 0.001, ****padj < 0.0001

Fig 3: GLP regulated the JAK2/STAT3 signaling pathway in RIR injury. (A) Schematic diagram of the JAK2/STAT3 signaling pathway by which GLP suppressed the microglia-mediated inflammation. (B) Representative bands for JAK2/STAT3 pathway proteins (P-JAK2, JAK2, P-STAT3, STAT3). (C–D) The phosphorylation levels of JAK2 and STAT3 were quantified using the ratios of P-JAK2/JAK2 and P-STAT3/STAT3, the results of which indicated that GLP inhibited the JAK2/STAT3 signaling pathway. All data were presented as mean ± SD (n = 3). #p < 0.05, ##p < 0.01 compared with the control group, ∗p < 0.05, ∗∗p < 0.01 compared with the RIR group.

Fig 4: JAK2/STAT3 pathway inhibition as potential mechanism of Ranunculus ternatus Thunb. For the treatment of breast cancer.

Fig 5: Effect of RRTS on JAK2/STAT3 pathway. (A–C) Relative protein expression of p-JAK2, p-STAT3, JAK2, and STAT3. (D) mRNA expression of JAK2 and STAT3. Data were shown as the mean ± SD of three independent experiments. Compared with control group, *P < 0.05, **P < 0.01.