Immunofluorescence, Extracellular: (Left) COS-7 cells transiently transfected human PD-1 were stained with PD-1 Monoclonal Antibody (CSB0023) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (Top right) COS-7 cells transiently transfected with an empty control vector stained with PD-1 Monoclonal Antibody. (Bottom right) Isotype control: COS-7 cells transfected with human PD-1 and stained with control MAb.

Immunofluorescence, Intracellular: (Left) COS-7 cells transiently transfected human PD-1 were permeabilized and stained with PD-1 Monoclonal Antibody (CSB0023) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (Top right) COS-7 cells transiently transfected with an empty control vector stained with PD-1 Monoclonal Antibody. (Bottom right) Isotype control: COS-7 cells transfected with human PD-1 and stained with control MAb.

Membrane Proteome Specificity: The specificity of PD-1 Monoclonal Antibody (CSB0023) was tested on the Membrane Proteome Arrayâ„¢ and shown to be specific for human PD-1. The Membrane Proteome Arrayâ„¢ contains 6,000 different human membrane proteins, each expressed in unfixed human cells to ensure native conformation and post-translational modifications. The Membrane Proteome Arrayâ„¢ represents the industry standard for determining the binding specificity of antibodies and other protein ligands.