Flow Cytometry, Extracellular: HEK-293F cells transiently transfected with human CD4 were stained with CD4 Monoclonal Antibody (CSB0118) (green) or isotype control antibody (gray), followed by AlexaFluor 647- conjugated anti-Mouse IgG secondary antibody. HEK-293F cells transiently transfected with an empty control vector were also stained with CD4 Monoclonal Antibody (CSB0118) (blue).
Immunofluorescence, Extracellular: (Left) COS-7 cells transiently transfected human CD4 were stained with CD4 Monoclonal Antibody (CSB0118) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (Top right) COS-7 cells transiently transfected with an empty control vector stained with CD4 Monoclonal Antibody. (Bottom right) Isotype control: COS-7 cells transfected with human CD4 and stained with control MAb.
Immunofluorescence, Intracellular: (Left) COS-7 cells transiently transfected human CD4 were permeabilized and stained with CD4 Monoclonal Antibody (CSB0118) followed by AlexaFluor 647 anti-Mouse IgG secondary antibody (red) and DAPI (blue). (Top right) COS-7 cells transiently transfected with an empty control vector stained with CD4 Monoclonal Antibody. (Bottom right) Isotype control: COS-7 cells transfected with human CD4 and stained with control MAb.
Membrane Proteome Specificity: The specificity of CD4 Monoclonal Antibody (CSB0118) was tested on the Membrane Proteome Arrayâ„¢ and shown to be specific for human CD4. The Membrane Proteome Arrayâ„¢ contains 6,000 different human membrane proteins, each expressed in unfixed human cells to ensure native conformation and post-translational modifications. The Membrane Proteome Arrayâ„¢ represents the industry standard for determining the binding specificity of antibodies and other protein ligands.
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