Anti-Histone H3 (citrulline R2 + R8 + R17) antibody [RM1001] ab281584 from Abcam

Product Specs
ItemAnti-Histone H3 (citrulline R2 + R8 + R17) antibody [RM1001]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab281584
Quantity1 mL, 20 µL, 100 µL
ApplicationsDot, Flow Cyt (Intra), ICC/IF, IP, PepArr, WB
Conjugate/TagUnconjugated
ReactivityHuman, Mouse, Rat
TargetH3C1 citrulline R2 + R8 + R17
ModificationsCitrulline-R17, Citrulline-R17, Citrulline-R17
CloneRM1001
HostRabbit
Antibody TypePrimary Antibody
Gene NameH3C1
ClonalityMulticlonal
Research AreaEpigenetics
IsotypeIgG
NCBI Full Gene NameH3 clustered histone 1
NCBI Gene AliasesH3/A, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, HIST1H3A

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Abcam

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Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Immunoregulatory properties of the human acellular amniotic membrane for diabetic wound healingiScienceAugust 21, 2026Rui-Peng Cai, Zhen Xiang, Lin-Jun Huang, Zhen-Yu Huang, Chao-Jun Zhuo, Kai Liu, Ornella Parolini, Yong-Can Huangtargeting MPO (Servicebio, GB11224, 1:5000), CD44 (Servicebio, GB112054, 1:3000), and CITH3 (Abcam, ab281584, 1:5000). Histological staining images were captured using an optical microscope (Leica DM4B), whereas

(2) S100A8/A9 Inhibition Reduces Neutrophil Extracellular Trap Formation and Mitigates Sepsis-Related Liver InjuryJ Inflamm ResAugust 14, 2026Yuye Zhang, Shiqi Yan, Jiayi Feng, Qiuyan Zhang, Junxian Xu, Xudong Han, Lijun Tianmembranes. The membranes were incubated overnight with primary antibodies, including antihistone H3 (1:1000, ab281584; Abcam, Cambridge, UK) and anti-NE (1:1000, ab131260; Abcam). Immunofluorescence Assay Liver tissue

(3) Protocol to investigate the role of NETosis in health and disease via a high-throughput neutrophil-like model using quantitative imagingSTAR ProtocAugust 5, 2026Gavin Li, Sara Versales, Brett Guadagnino, Kavya Krishnan, Michele Matsuoka, Hyesun Jang, Marcelo FreireRRID: AB_10596801" Anti-Histone H3 (citrulline R2 + R8 + R17) antibody [RM1001] (1:2000),Abcam,"Cat# ab281584, RRID: AB_3697176" "Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™

(4) Tunable Self-Assembling Cellular Microarray for Single-Neutrophil Vital and Suicidal Extracellular TrapsLab on a ChipJuly 6, 2026Jacob Doon-Ralls, Sophia Mayone, Xilal Y. Rima, Dharti Shantaram, Kim Truc Nguyen, Ajeet Singh, Bradley J. Needleman, Sabrena Noria, Stacy Brethauer, Kyle A. Perry, et al.AlexaFluor™ 488-conjugated anti-H3 Histone (citrulline R2 + R8 + R17, 1 : 100 dilution, Abcam Cat# ab281584), to allow for the real time quantification of suicidal NETosis. 4 nM PMA was chosen as 4 times the

(5) Spectral Flow Cytometry Method for Immunophenotyping Neutrophil Activation and NETs in an Acute Dust Exposure ModelImmunity, Inflammation and DiseaseJune 30, 2026Logan S. Dean, Maëlis J. L. Wahl, Pinaki Mondal, Tara M. Nordgren, Juwon ParkBSA in PBS with 0.02% Triton X‐100 at room temperature for 1 h. CitH3 (Abcam, Waltham, MA. Cat. No. ab281584) and MPO (R&D, Minneapolis, MN. Cat. No. AF3667) were diluted to 1:250 and 1:100 in the 0.5% blocking

(6) CSF2 drives radiation-induced lung injury by eliciting PADI4-high pathogenic neutrophilsJournal of Translational MedicineJune 10, 2026Yun Wang, Maidina Abulaihaiti, Yan Pan, Jianghong Zhang, Chunlin ShaoServicebio, China), and anti-citrullinated histone H3 (CitH3; citrulline R2 + R8+R17; clone RM1001, ab281584, Abcam). Alexa Fluor 488-conjugated anti-rabbit, Alexa Fluor 555-conjugated anti-rat, and Alexa Fluor

(7) Clitocine suppresses TNBC progression by boosting CCRL2 to block survival signals and neutrophil-driven inflammationJournal of Biological EngineeringJune 8, 2026Qi Chen, Yanyun Ruan, Shuanshuan Liu, Hozeifa M. Hassan, Shaoyin Bao, Weiliang Liu, Jianguo Sun, Hongsheng LuName,Brand,Number,Application N-cadherin,proteintech,22018-1-AP,WB Vimentin,CST,5741,WB E-cadherin,proteitech,20874-1-AP,WB c-PARP,CST,9541P,WB c-Caspase8,CST,9496,WB c-Caspase9,CST,9505,WB CCRL2,proteintech,66611-1-PBS,WB&IHC chemerin,proteintech,10216-1-AP,IHC TLR4,Affinity,AF7017,WB&IHC p-IKKα,CST,2697T,WB&IHC IKKα,CST,2682P,WB TRAF6,CST,8028T,WB p-PI3K,Affinity,AF3242,WB PI3K,proteintech,60225-1-Ig,WB p-AKT,proteintech,80455-1-RR,WB&IHC AKT,proteintech,10176-2-AP,WB p-NFκB,CST,3033T,WB p-NFκB,Affinity,AF3219,IHC NFκB,CST,8242T,WB p-ERK,proteintech,28733-1-AP,WB&IHC ERK,proteintech,20874-1-AP,WB Flag,CST,"14,793",WB MPO,Abcam,ab208670,IF CitH,Abcam,ab281584,IF "Anti-rabbit IgG, HRP-linked Antibody",CST,7074P2,WB "Anti-mouse IgG, HRP-linked Antibody",CST,7076P2,WB Goat

(8) Hypoxia‐preconditioned adipose‐derived mesenchymal stem cells‐derived exosomes transferring H19 obstruct neutrophil extracellular traps formation via HOXA5 ‐mediated inactivation of TLR4 / NF ‐ κB / NLRP3 inflammatory signalingJournal of Diabetes InvestigationJune 8, 2026Li Qian, Xianxi Meng, Bairong Fang, Li Piparaffin sections were incubated with the marker antibodies for NETs, including anti‐CitH3 (1:50, #ab281584, Abcam, Cambridge, UK) and anti‐Myeloperoxidase (MPO, 1:50, #ab45977, Abcam), as well as the marker

(9) Mechanistic and functional characterization of NETs/IL-17 as a therapeutic target in EMT and brain metastasis of lung adenocarcinomaFrontiers in ImmunologyMay 25, 2026Yong Cai, Bin Su, Jiying Wangthen incubated overnight at 4 C with anti-MPO (1:50, 66177-1-Ig, Proteintech) and anti-CitH3 (1:2000, ab281584, Abcam) primary antibodies. Subsequently, samples were treated with AF488-conjugated goat anti-rabbit

(10) S100A9 deletion ameliorates acute lung injury by attenuating excessive neutrophil activation via activating the NRF2/HO-1 signaling axisRespiratory ResearchMay 22, 2026Shuang Yin, Xinyu Hu, Hao Cai, Ruoxuan Jiang, Jiali Li, Yan ChengUSA). Anti-myeloperoxidase (MPO, Cat. No. ab208670) and anti-citrullinated histone H3 (CitH3, Cat. No. ab281584) antibodies were obtained from Abcam (Cambridge, UK). Antibodies against interleukin-6 (IL-6, Cat. No.

(11) Activated Platelet-Released Heat Shock Protein 90α Triggers Autophagy-Dependent Neutrophil Extracellular Trap Formation and Amplifies SepsisAdvanced science (Weinheim, Baden-Wurttemberg, Germany)May 1, 2026Wang C, Leng M, Sun C, Cao J, Li L, Jia Y, Han Y, Liu Y, Zhang Y, Zhang C, et al.G1231). After antigen repair again, the anti‐Histone H3 (citrulline R2 + R8 + R17) antibody (Abcam, ab281584) was incubated, followed by sequential incubation with secondary antibodies and iF647‐Tyramide dye (Servicebio,

(12) Lipid asymmetry disruption by XKR8 orchestrates neutrophil extracellular trap formation and inhibits fungal infectionNature immunologyMay 1, 2026Liu W, Ping J, Deng L, Wang K, Xu J, Peng N, Yang M, Tang H, Liu Z, Wang W, et al.(LLSC20230277). Antibodies and reagents Anti-citrullinated histone H3 (citrulline R2 + R8 + R17) (ab5103, 1:1,000 dilution; ab281584, 1:1,000 dilution) and anti-neutrophil elastase (ab314916, 1:100 dilution) were purchased from Abcam.

(13) Integrated pharmacology and experimental validation reveals potential multiple mechanisms of the neutrophil elastase inhibitor sivelestat in attenuating myocarditisFrontiers in PharmacologyApril 30, 2026Ruguo Ren, Ning Wang, Chen Chen, Miao Yang, Bin Li, Yuxuan Yang, Hang Zhang, Bo Yang, Yu Wu, Wei Gao, et al.identify NETs, mouse myocardial tissue samples were immunostained for citrullinated histone 3 (Abcam, #ab281584, 1:1000) and Neutrophil Elastase (Affinity, #AF0010, 1:100). The samples were fixed with 4% paraformaldehyde,

(14) PD-1 deficiency exacerbates Mycobacteroides abscessus lung infection via metabolic rewiring and dysregulated neutrophil/T cell responsesFrontiers in ImmunologyApril 24, 2026Xiaowen Yu, Lu Huang, Jianping Xie(1:3000, GB11229, Servicebio, China), MPO (1:5000, GB150006, Servicebio, China), and H3cit (1:3000, AB281584, Abcam, USA). Primary antibodies were incubated at 37°C for 1 h. Secondary antibodies (Servicebio, China)

(15) Hub genes of neutrophil extracellular traps in abdominal aortic aneurysm: a bioinformatics analysisHereditasApril 20, 2026Jinxin Liu, Bixuan Yue, Zhiying Shen, Bo Yang, Yuxuan Ou, Xiaowu Wang(Abmart, TD6685S), MMP9 (Abcam, ab283575), MPO (R&D, AF3667), citrullinated histone H3 (CitH3) (Abcam, ab281584), IL6 (Wanleibio, WL02841), and GAPDH (Proteintech, 60004-1-Ig) at 4 °C overnight and with corresponding

(16) Development of motif-specific monoclonal antibodies for global protein citrullination detection with minimal cross-reactivity to homocitrullinationCell Reports MethodsApril 20, 2026Sophia Laposchan, Erik Riedel, Andrew Flatley, Selina Pasquero, Regina Feederle, Chien-Yun LeeWB Abcam,ab212082,histone H3 (cit R26),rabbit (IgG),monoclonal [EPR20606],not disclosed,"Dot, ELISA, and WB" Abcam,ab281584,histone H3 (cit R2+ cit R8 + cit R17),rabbit (IgG),multiclonal [RM1001],not disclosed,"Dot, Flow Cyt

(17) Integration of bioinformatics analysis and experimental verification reveals the role of neutrophil extracellular traps in silica nanoparticles-induced lung injuryJournal of NanobiotechnologyApril 14, 2026Xiu He, Xiaoli Yuan, Xuedong Sa, Jiaqi Ban, Shuai Fu, Mingdan You, Jun Li(China). The following primary antibodies were used in this study: anti-αM (ab128797) and anti-CitH3 (ab281584) was purchased from Abcam (Cambridge, MA, USA); anti-αL (abs115674), anti-IL-18 (abs120003) ,and anti-IL-6

(18) Galectin-9high Neutrophils Exacerbate Radiation-Induced FrailtyAging CellApril 7, 2026Zhuo Cheng, Le Ma, Yan Chen, Jialun Li, Yali Dai, Wentao Xie, Yingjie Li, Tianlang Xu, Zhihe Hu, Weilin Huang, et al.were Gal‐9 (1:500, Abcam #ab69630), Myeloperoxidase (1:50, Abcam #ab90810), Histone H3 (1:1000, Abcam #ab281584), Ly6g + Ly6c (1:500, Abcam #ab25377), and CD47 (1:500, Abmart #T55251S). 2.3 Flow Cytometry Analysis Mouse

(19) Citrullinated histone H3 identifies neutrophil extracellular trap formation and correlates with renal disease activity in ANCA-associated vasculitisClin Kidney JApril 6, 2026Natsumi Kamijo, Akiko Mii, Takashi Tani, Rei Nakazato, Arimi Ishikawa, Tetsuya Kashiwagi, Ryuji Ohashi, Masataka Kuwana, Akira Shimizu, Yukinao Sakai, et al.formalin-fixed, paraffin-embedded (FFPE) renal biopsy specimens. For H3Cit, two rabbit antibodies, ab281584 (Abcam, Cambridge, UK) and #97272 (Cell Signaling Technology, Danvers, MA, USA), were initially evaluated

(20) Orally Administered Rhamnan Sulfate from Monostroma nitidum Significantly Inhibits Melanoma Metastasis in Lungs and Aorta of Mice Implanted with B16 CellsMarine DrugsMarch 29, 2026Keiichi Hiramoto, Masashi Imai, Masahiro Terasawa, Koji Suzuki1:1000; 551459, BD Biosciences, Franklin Lakes, NJ, USA), citH3 (neutrophil activation marker, 1:1000; ab281584, Abcam), PAD4 (neutrophil activation marker, 1:1000; ab214810, Abcam), and β-actin (1:5000; #58169,

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Anti-Histone H3 (citrulline R2 + R8 + R17) antibody [RM1001] from Abcam

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Anti-Histone H3 (citrulline R2 + R8 + R17) antibody [RM1001] from Abcam

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