Western blot analysis of NF-H in Different tissue lysates. Samples were incubated in NF-H polyclonal antibody (Product # PA3-16721 using a dilution of 1:10,000. Antibody in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord [4] mouse brain, and [5] mouse spinal cord lysate. Strong band at about 220 kDa corresponds to the phosphorylated axonal form of the NF-H subunit. Smaller proteolytic fragments of NF-H are also detected with RPCA-NF-H antibody.

Section of rat spinal cord stained with PA3-16721 (green) and monoclonal antibody to ubiquitin C-terminal hydrolase 1 (UCHL1) MA1-46079 (red). The neurofilament NF-H antibody binds primarily to phosphorylated axonal forms of NF-H, and so stains axons coursing between the large UCHL1 positive neurons. These large cells are a-motorneurons and UCHL1 protein is a major component of the perikarya and dendrites of these cells.