Western Blot analysis in HT-1080 whole cell lysate (20ug) using 034316 (1:2000). Secondary: IgG, (H+L) (HRP) goat anti-rabbit (1:10,000). Predicted band size: 18kD

Western Blot analysis using 034316 (1:1000) Lane 1: HT-1080 whole cell lysate Lane 2: U266B1 whole cell lysate Lysates/proteins at 20ug/lane. Secondary: IgG (HRP) (H+L) goat anti-rabbit IgG (1:10,000). Predicted band size: 18kD. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemistry analysis of CTAG1A in human testis tissue sections using 034316 (1:25). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5hrs at RT; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with 034316 (1:25) for 1hr at 37°C. An undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Immunohistochemistry analysis of CTAG1A in human gastric carcinoma tissue sections using 034316 (1:250). Samples were incubated with 034316 (1:250) for 1hr at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.