Western Blot analysis of lysate from SH-SY5Y cell line (35ug), using 041591 (1:1000). A goat anti-rabbit IgG H&L (HRP) at 1:5000 was used as the secondary antibody.

Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue using 041591 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of 041591 for immunohistochemistry.

Confocal immunofluorescent analysis of HepG2 cells using 041591 followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Flow Cytometric analysis of HepG2 cells using 041591 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.