Western blot of DARPP-32 in rat striatal lysate showing specific immunolabeling of a band at ~32 kDa corresponding to Phospho-DARPP-32 (Thr34) polyclonal antibody (Product # PA1-4609) in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (1,200 units for 30 min).