Cleaved Caspase-8 (Asp374) (18C8) Rabbit mAb 9496S from Cell Signaling Technology

Product Specs
ItemCleaved Caspase-8 (Asp374) (18C8) Rabbit mAb
CompanyCell Signaling Technology
Price $401.00 Inquire
Catalog Number9496S
Quantity100 µl
ApplicationsW
ReactivityH
TargetCasp8 (Asp374) cleaved
IsotypeIgG
HostRabbit
NCBI Gene AliasesALPS2B, CAP4, Casp-8, FLICE, MACH, MCH5
NCBI Full Gene Namecaspase 8
Clone18C8
Molecular Weight18, 41, 43
ClonalityMonoclonal
Research AreaApoptosis

Questions and Answers

Can this antibody be used to study apoptosis in human cells?

Yes, the Cleaved Caspase-8 (Asp391) Rabbit mAb has been shown to be effective in detecting cleaved caspase-8, a key marker of apoptosis, in various studies. For instance, it was utilized in research examining the apoptotic effects of different treatments on cancer cells, highlighting its relevance in apoptosis studies (, ).

What specific applications has this antibody been used for in scientific research?

The Cleaved Caspase-8 antibody has been primarily used in Western Blot applications to analyze apoptosis-related pathways. It has been cited in studies investigating the mechanisms of cell death in various contexts, including cancer therapy and cellular responses to stress (, ).

Is this antibody suitable for use in immunoblotting of human tissues?

Yes, the Cleaved Caspase-8 antibody has been successfully employed in Western Blot analyses of human tissues, as indicated by its use in studies examining apoptosis in human placental tissues and cancer cell lines (, ).

What is the significance of detecting cleaved caspase-8 in experimental models?

Detecting cleaved caspase-8 is crucial for understanding the apoptotic process, as it indicates the activation of the caspase cascade, which is a hallmark of programmed cell death. This antibody has been referenced in studies that elucidate the role of caspase-8 in various apoptotic pathways, making it a valuable tool for researchers studying cell death mechanisms (, ).

Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) GO-Y078 Triggers Program Cell Death in Human Cervical Cancer Cells Via MAPK Activation-Dependent Apoptotic Caspases SignalingIntegrative Cancer TherapiesApril 30, 2026Chung-Yuan Lee, Po-Hui Wang, Yi-Hsien Hsieh, Yi-Hsuan Hsiao, Chih-Hsin Tang, Tzu-Yu Huang, Shun-Fa Yang, Pei-Ni Chen(ADP-ribose) polymerase (PARP; CST, Cat #9542, RRID: AB_2160739), anti-cleaved caspase-8 (CST, Cat #9496, RRID: AB_561381), anti-cleaved caspase-9 (CST, Cat #9505, RRID: AB_2290727), anti-cleaved caspase-3

(2) Cryptocaryone Exhibits ROS/MAPK-Dependent Antiproliferative and Apoptosis-Inducing Effects on Triple-Negative Breast Cancer Cells and Proof-of-Concept Breast Cancer Mouse ModelDrug Dev ResApril 19, 2026Ya-Ting Chuang, Wangta Liu, Tsu-Ming Chien, Ammad Ahmad Farooqi, Hsun-Shuo Chang, Jun-Ping Shiau, Hsueh-Wei ChangAntibodies (1:1000) were derived from an apoptosis sampler antibody kit [#9915] and a cleaved caspase 8 [#9496] (Cell Signaling Technology), accompanied by the internal control antibody GAPDH (1:10000) [#GT239]

(3) Antigenic cancer persister cells survive direct T cell attackbioRxivApril 12, 2026Michael X. Wang, Brandon E. Mauch, August F. Williams, Tania Barazande-Pour, Filipe Araujo Araujo Hoffmann, Sophie H. Harris, Cooper P. Lathrop, Claire E. Turkal, Bryan S. Yung, Michelle H. Paw, et al.(CST #9176, 1:1000), PD-L1 (CST #13684, 1:1000), IDO-1 (CST #86630, 1:1000), Cleaved Caspase 8 (CST #9496, 1:1000), Cleaved Caspase 7 (CST #9491, 1:1000), Cleaved Caspase 3 (CST #9664, 1:1000), Phospho-Histone

(4) Lipid droplet-induced T cell death sustains autoimmune tissue inflammationCell MetabolismApril 7, 2026Jitendra Kumar, Yoshinori Takashima, Jose Morales, Brandon Wong, Lina Werner, Isabel N. Goronzy, Selene Rubino, Robert T. Trousdale, Gerald J. Berry, Jörg J. Goronzy, et al.,Cell Signaling Technology,#8438; P55210 Caspase-8 Cleaved (Asp374) (18C8) Rabbit mAb,Cell Signaling Technology,#9496; Q14790 CD44 Antibody,Cell Signaling Technology,#3578: P16070 Cleaved Gasdermin D (Asp275) (E7H9G) Rabbit mAb

(5) UPR/ATF4/Noxa pathway overactivation through SERCA2 inhibition or ONC201 treatment combined with ABT-737 triggers apoptosis in chemoresistant ovarian cancer cells and patient-derived tumor organoidsCell Death & DiseaseMarch 27, 2026Sahra Messaoudi, Romane Florent, Louis-Bastien Weiswald, Guillaume Desmartin, Lucie Lecouflet, Steven Lohard, Léa Grunchec, Edwige Abeilard, Marilyne Guillamin, Jordane Divoux, et al.(#2774), anti-BCL-xL (#2764), anti-BIM (#2819), anti-BiP (#3177), anti-CASPASE 3 (#9662), anti-CASPASE 8 (#9496), anti-CHOP (#2895), anti-DR5 (#8074), anti-MCL-1 (#5453), anti-PARP (#9542), anti-PUMA (#12450) (Cell Signaling Technology

(6) Macrophage metabolic exhaustion and PANoptotic cell death drive chronic tissue inflammation in rheumatoid arthritisImmunityMarch 6, 2026Tao Huang, Yoshinori Takashima, Jitendra Kumar, Jose Morales, Kevin Le, Zhensheng Hu, Selene Rubino, Robert T. Trousdale, Gerald J. Berry, Jorg J. Goronzy, et al.(KP1) (ThermoFisher Scientific, 14–0688-82), cleaved caspase-7 (CST, 9491S), cleaved caspase-8 (CST, 9496S), goat anti-rat IgG (H+L) Cyanine3 (Invitrogen, A10522), donkey anti-sheep IgG (H+L) Alexa Fluor 647

(7) Notch-1 in macrophages promoted the ischemia-reperfusion via modulating EZH2/HSF1/BRD4/SIRPα/SHP2 induced ROS and apoptosis in cardiomyocyteScientific ReportsFebruary 25, 2026Chengbi Tong, JiaWei Zhang, YaJing Zuo, XiaoTong Yu, ShaSha Zang, Sha Liucleaved-caspase3(CST, #9664, 1:1000), Caspase-8(CST, #4790, 1:1000), GAPDH(CST, #2118, 1:2000) cleaved-caspase8(CST, #9496, 1:1000), and PCNA(CST, #13110, 1:1000) at 4 °C overnight. After adding secondary antibodies tagged

(8) Caspase-activation powers anti-Desmoglein 3-induced acantholysis in human epidermisCell Death DiscoveryFebruary 19, 2026Morna F. Schmidt, Maria A. Feoktistova, Diana Panayotova-Dimitrova, Eva Miriam Buhl, Peter Boor, Tim Ruhl, Jens Waschke, Ritva Tikkanen, Martin Röcken, Jens M. Baron, et al.Mouse IgG1 Alexa Fluor® 488-conjugated Antibody (IC002G, R&D, Minneapolis, USA), Cleaved caspase-8 (#9496, Cell Signaling, Danvers, USA), anti-Desmoglein 3 antibody (ab183743, Abcam, Cambridge, United Kingdom).

(9) A Novel Pyrazole Pyrimidine Derivative MBP346 Induces Cell Death via ROS-Mediated Mitochondrial Damage in Human Head and Neck Squamous Cell CarcinomaMoleculesFebruary 17, 2026Chunsheng Hu, Pengcheng Xu, Juan Hu, Bo Fang, Jiangping Meng, Yan Tang(9532, CST, Danvers, MA, USA), Cleaved-Caspase 3 (9664, CST, Danvers, MA, USA), Cleaved-Caspase 8 (9496, CST, Danvers, MA, USA), MCL1 (220062, Zen BIO, Chengdu, China), Bcl2 (12789-1-AP, Proteintech, Wuhan,

(10) Ebastine targets HER2/HER3 signaling and cancer stem cell traits to overcome trastuzumab resistance in HER2‑positive breast cancerInt J Mol MedFebruary 2, 2026Eunsun Jung, Ji Young Kim, Dongmi Ko, Juyeon Seo, Sang Yoon Lee, Daeun Lee, Subeen Kim, Minsu Park, Seongjae Kim, Soeun Park, et al.(Cell Signaling Technology, Inc.; cat. no. 8438) and -8 (Cell Signaling Technology, Inc.; cat. no. 9496), Bax (Cell Signaling Technology, Inc.; cat. no. 2772) and vimentin (Cell Signaling Technology, Inc.;

(11) Epithelial Gab1 Restricts Sepsis‐Induced Intestinal Injury by Orchestrating TNF/NF‐κB AxisMediators of InflammationJanuary 31, 2026Wei Jin, Yanchuang Wu, Xiaoqing Cheng, Yu Pan, Lifeng He, Yun Xu, Jiaqi Xu, Xue Zhang, Hengrui Liu14064‐1‐AP, 1:1000) cl‐Caspase3 (CST, 9664, 1:1000), Caspase3 (CST, 9662, 1:1000), cl‐Caspase8 (CST, 9496, 1:1000), Caspase8 (CST, 4790, 1:1000), Bcl‐2 (Proteintech, 12789‐1‐AP, 1:1000), Bax (Proteintech, 50599‐2‐Ig,

(12) EGCG promotes apoptosis in BT-549 triple-negative breast cancer cells by targeting STAT3Translational Cancer ResearchJanuary 31, 2026Ke Zhang, Xinghua Tan, Xufeng Zhou, Lushan Yu, Zhihua Tangcaspase-3 (CST, 9662), cleaved caspase-3 (CST,9661), caspase-8 (CST, 4790), cleaved caspase-8 (CST, 9496), ERK (CST, 4695), p-ERK (CST, 5726), JAK (CST, 3332), p-JAK (CST,3331), GAPDH (CST, 2118). Flow cytometry

(13) Homoisoflavanone Delays Colorectal Cancer Progression via DNA Damage‐Induced Mitochondrial Apoptosis and Parthanatos‐Like Cell DeathAdvanced ScienceJanuary 28, 2026Hongjie Fan, Huzi Zhao, Pei Zhang, Pengfei Yu, Yunfei Ji, Gang Chen, Hongli Jin, Yanfang Liu, Jin Liu, Zhe‐Sheng Chen, et al.antibodies against the following proteins: cleaved Caspase 3 (25128‐1‐AP, Proteintech), cleaved Caspase 8 (9496, CST), PARP (9542, CST), cleaved PARP (9541, CST), P21 (2947, CST), P53 (2527, CST), Cyclin A2 (91500,

(14) Pharmacologic Modulation of the PAR-2–ERK Axis by Statins Converts Inflammatory Survival Signalling into Apoptosis in Colorectal Cancer CellsInternational Journal of Molecular SciencesJanuary 16, 2026Layla Amiri, Rajashree Patnaik, Riah Lee Varghese, Bintul Huda, Yajnavalka Banerjeecleaved caspase-3 (25128-1-AP, Proteintech), pro-caspase-8 (13423-1-AP, Proteintech), cleaved caspase-8 (9496T, Cell Signaling), BCL-2 (3498T, Cell Signaling), BAX (50599-2-Ig, Proteintech), and GAPDH (2118T, Cell Signaling

(15) A Novel Autophagy Inhibitor p -Hydroxylcinnamaldehyde Suppresses Esophageal Squamous Cell Carcinoma by Targeting LDHA Phosphorylation-Mediated Metabolic ReprogrammingResearch (Wash D C)January 12, 2026Sisi Wei, Jingjing Wang, Zhe Zhang, Yuhui Fu, Leyang Zhao, Yanna Bi, Xiaoya Li, Suli Dai, Cong Zhang, Wenjiao Zhu, et al.and antibodies Antibodies against cleaved caspase-3 (#9664), caspase-3 (#9662), cleaved caspase-8 (#9496), caspase-8 (#4790), cleaved caspase-9 (#9505), caspase-9 (#9508), p-AMPK (#2535), and p-ULK1 (#14202)

(16) Targeting FOXM1 regulates metabolic signatures through ROS-dependent JNK/Bmi1/Skp2 axis in human cutaneous T-cell lymphomaCell Death & DiseaseJanuary 7, 2026Abdul Q. Khan, Maha Agha, Fareed Ahmad, Rasheeda Anver, Majid Alam, Joerg Buddenkotte, Shahab Uddin, Martin Steinhoff9252), FOXM1 (Cat# 20459), Caspase-3 (Cat# 9662), Cleaved caspase-3 (Cat# 9661), Cleaved caspase-8 (Cat# 9496), Skp2 (Cat# 4358), Bmi1(Cat# 6964), p21 (Cat# 64016), PARP (Cat# 9542), Cleaved PARP (Cat# 9544), LC3A/B

(17) Betulinic Acid Suppresses UBE2T Expression via MAPK/ERK Inhibition to Block FANCI and FANCD2 Monoubiquitination in GlioblastomaJ Cell Mol MedJanuary 4, 2026Yifeng Bao, Maode WangNo. 8033), UAF1 (Abcam, Cat. No. ab122473), Pro‐Caspase 8 (Asp374), p43/p41 and p18 (CST, Cat. No. 9496), YY1 (CST, Cat. No. 46395), Lamin B1 (CST, Cat. No. 13435), ERβ (CST, Cat. No. 89954), pERK1/2 (Thr202/Tyr204)

(18) The PI3K Inhibitor HCD Promotes Caspase Activation in Head and Neck Squamous Cells by Upregulating the Extrinsic and Intrinsic Apoptosis PathwaysJournal of Oral Pathology & MedicineJanuary 1, 2026Leong‐Perng Chan, Ya‐Ping Tseng, Hui‐Ching Wang, Chen‐Yu Chien, Che‐Wei Wu, Ling‐Feng Wang, Tung‐Wen Yen, Po‐Chun Wang, Chih‐Chuang Liaw, Chia‐Hua Liang(Santa Cruz Biotechnology, sc‐17784, RRID: AB_673489), cleaved Caspase‐8 (Cell signaling technology, #9496, RRID: AB_561381, 1:1000), cleaved Caspase‐3 (Cell signaling technology, #9661, RRID: AB_2341188, 1:1000),

(19) FOXM1 down-regulation is a trigger of isatuximab-induced direct cell death in multiple myeloma cells with 1q+HaematologicaJanuary 1, 2026Jiro Kikuchi, Naoki Osada, Sae Matsuoka, Tomomi Ohta, Hirotaka Kazama, Heigoro Shirai, Marco Meloni, Marielle Chiron, Hiroshi Yasui, Hideki Nakasone, et al.standard method using the following antibodies: anti-cleaved caspase-3 (#9661), anti-cleaved caspase-8 (#9496), and anti-cleaved PARP (#9541) (Cell Signaling Technology). Lower panel: reactice oxygen species (ROS)

(20) Downregulation of Engulfment and cell motility 1 (Elmo1) induces quiescence and resistance to poly(I:C)-induced apoptosis in endothelial cellsCell Death & DiseaseDecember 20, 2025Yukako Kayashima, Anshulika A. Deshmukh, Yuki Kiyokawa, Niroshani M. W. Wariyapperuma Appuhamillage, Jiayi Zhou, Mohamed-Yahia S. Monawar, Melanie Nassar-Guifarro, Feng Li, Nobuyo Maeda(LI-COR, Lincoln, NE, USA). Primary antibodies against Elmo1 (1:1000, #14457), cleaved caspase-8 (1:1000, #9496), cleaved caspase-3 (1:1000, #9661), caspase-3 (1:1000, #9662), and caspase-7 (1:1000, #9492) were purchased

Write a Review

(1)

2/9/2024Apoptotic Marker From Cell Signaling Gives Great Western Blot Results Gherardo BaudoI used the Cleaved Caspase-8 antibody in my study on neuronal cells stimulated with beta-amyloid to induce Alzheimer's. The antibody reliably detected apoptotic markers, providing consistent and ... Read Review

Cleaved Caspase-8 (Asp374) (18C8) Rabbit mAb

Cleaved Caspase-8 (Asp374) (18C8) Rabbit mAb from Cell Signaling Technology

Description

Questions and Answers

Cell Signaling Technology

Citations (367)

Reviews

Cleaved Caspase-8 (Asp374) (18C8) Rabbit mAb from Cell Signaling Technology

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map