Apoptotic Marker From Cell Signaling Gives Great Western Blot Results

Houston Methodist Research Institute

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Cell Signaling Technology

Product Name:

Cleaved Caspase-8 (Asp374) (18C8) Rabbit mAb

Catalog Number:

9496S

Clone Number:

I used the Cleaved Caspase-8 antibody in my study on neuronal cells stimulated with beta-amyloid to induce Alzheimer's. The antibody reliably detected apoptotic markers, providing consistent and reproducible results.

The Western blot process, including overnight incubation at 4°C with 5% BSA in 1xTBST, followed by three washes and a 2-hour incubation with the secondary antibody, proved efficient in signal detection. The antibody's reliability and straightforward protocol make it a valuable tool for Alzheimer 's-related apoptosis studies. Highly recommended for researchers in the field.

Experimental Design and Results Summary

Applications

Western blot

Sample

Neuronal cells

Primary Incubation

1:1000 overnight

Blocking Agent

5% BSA in TBST at 4 degrees C

Secondary Incubation

1:1000

Tertiary Incubation

N/A

Detection

Chemiluminescence

Results Summary

I used the Cleaved Caspase-8 antibody in my study on neuronal cells stimulated with beta-amyloid to induce Alzheimer's. The antibody reliably detected apoptotic markers, providing consistent and reproducible results.The Western blot process, including overnight incubation at 4°C with 5% BSA in 1xTBST, followed by three washes and a 2-hour incubation with the secondary antibody, proved efficient in signal detection. The antibody's reliability and straightforward protocol make it a valuable tool for Alzheimer's-related apoptosis studies.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

High quality.

The Bad

N/A

The Bottom Line

Highly recommended for researchers in the field.

Share your experience with other scientists. Write a Review! »

Join the discussion