Histone H3 (D1H2) XP ® Rabbit mAb 4499L from Cell Signaling Technology

Product Specs
ItemHistone H3 (D1H2) XP ® Rabbit mAb
CompanyCell Signaling Technology
Price $911.00 Inquire
Catalog Number4499L
Quantity300 µl
ApplicationsW, IHC-P, IF-IC, FC-FP
ReactivityH, M, R, Mk
TargetH3
HostRabbit
NCBI Gene AliasesH3/A, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, HIST1H3A
ClonalityMonoclonal
NCBI Full Gene NameH3 clustered histone 1
Research AreaChromatin Regulation / Nuclear Function
IsotypeIgG
CloneD1H2
Molecular Weight17

Questions and Answers

What applications has the Histone H3 (D1H2) XP Rabbit mAb been used for in scientific research?

The Histone H3 (D1H2) XP Rabbit mAb has been shown to work in various applications including Western Blotting, Flow Cytometry, Immunocytochemistry, Immunofluorescence, and Immunohistochemistry. It has been utilized in studies to analyze histone modifications and their roles in gene regulation and cellular processes.

Can this antibody be used to study the effects of toxic substances on protein synthesis?

Yes, the Histone H3 (D1H2) XP Rabbit mAb has been referenced in studies examining the effects of toxic substances, such as Thallium(I), on protein synthesis. For instance, in one study, it was used to assess the impact of Thallium(I) treatment on histone levels and ribosomal function, highlighting its utility in understanding cellular responses to environmental toxins ().

Is this antibody suitable for use in human cell lines, and what evidence supports this?

The Histone H3 (D1H2) XP Rabbit mAb is suitable for use in human cell lines, as it has been cited in multiple studies involving human cells, including those investigating the mechanisms of cancer and gene expression regulation. Its reactivity with human samples has been confirmed through various applications in published research.

How has this antibody contributed to understanding histone modifications in cancer research?

The Histone H3 (D1H2) XP Rabbit mAb has been utilized in cancer research to investigate histone modifications, such as H3K27 methylation, which are critical for understanding tumorigenesis. For example, studies have shown that mutations in histone H3 can inhibit Polycomb Repressive Complex 2 activity, affecting gene expression in cancer cells ().

Cell Signaling Technology

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Phone: 978-867-2388
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Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Damage to newly synthesized proteins is a major cause of Cd(II) toxicity counteracted by proteasomes and integrated stress response in human cellsChemico-Biological InteractionsJune 1, 2026Giorgiana Madalina Ursu, Anatoly Zhitkovich11815), SOD1 (Cell Signaling, 2770), fibrillarin (Cell Signaling, 2639), histone H3 (Cell Signaling, 4499), HK2 (Cell Signaling, 2106), RRM1 (Cell Signaling, 3388), XPA (Santa Cruz, sc-56813). Horseradish peroxidase-conjugated

(2) KDM6B safeguards mineralized tissue homeostasis from mechanical stress through epigenetic control of PIEZO1-mediated mechanotransduction in the mouse incisorBone ResMay 28, 2026Lin Meng, Mingyi Zhang, Jifan Feng, Tingwei Guo, Hana Hekmat, Heliya Ziaei, Peng Chen, Aaron Harouni, Thach-Vu Ho, Yang ChaiCell Signaling Technology, 9733); rabbit anti-Histone H3 (D1H2) (1:1 000, Cell Signaling Technology, 4499); mouse anti-CaMKII (phospho T286) antibody [22B1] (1:500, Abcam, ab171095); mouse anti-CaMKII antibody

(3) CXCL6 exacerbates metabolic dysfunction-associated steatohepatitis by suppressing LPIN1-mediated fatty acid oxidation in hepatocytesInt J Biol SciMay 1, 2026Ye Eun Cho, Min-Ju Kim, Yeonsoo Kim, Hyeokjin Lim, Yunseo Park, Sangok Kim, Seung-Jin Kim, Jin-Wook Yoo, Seungjin Ryu, Parkyong Song, et al.(#9216), p38 (#8690), phospho-JNK (#4668), JNK (#9252), LPIN1 (#14906), Lamin A/C (#4777), histone H3 (#4499), and α-tubulin (#2144) were obtained from Cell Signaling Technology (Danvers, MA, USA). Enzyme-linked immunosorbent assay

(4) L-2-hydroxyglutarate impairs neuronal differentiation through epigenetic activation of MYC expressionJournal of Clinical InvestigationMay 1, 2026Wen Gu, Xun Wang, Ashley Solmonson, Ling Cai, Yi Xiao, Alpaslan Tasdogan, Jordan Franklin, Yuannyu Zhang, Hua Zhang, Aundrea K. Westfall, et al.used 2 μg of histone extract. Histone H3 was used as a loading control (Cell Signaling Technology, 4499). DNA dot blot analysis. DNA dot blot analysis was performed using a Bio-Dot Microfiltration Apparatus

(5) Long-lived mammals contain more phosphorylation sites in the SIRT6 C-terminus that enhance PARP1 interaction and resistance to oxidative stressbioRxivApril 30, 2026Jonathan Gigas, Michael E. Meadow, Jing Guo, Catherine Lan, Eric Hillpot, John C. Martinez, Gregory Tombline, Philip Bellomio, Valeria Rivera Almodovar, Kevin A. Welle, et al.membranes blocked with 5% nonfat dry milk. Antibodies used are as follows: SIRT6 CST cat#12486, H3 CST cat#4499, p-mTOR CST cat#5536, PARP1 Thermo cat#13371–1-AP, Ku70 Abcam cat#ab202022, p-Rb CST cat#9308S, γ-H2AX

(6) Sphingosine-1-Phosphate Promotes FOS Activation in Osteosarcoma Under Tumor AcidosisActa Physiol (Oxf)April 26, 2026Nicolò Bozzini, Margherita Cortini, Alberto Righi, Agamemnon E. Grigoriadis, Michael Dack, Elizabeta Ilieva, Federica Torricelli, Veronica Manicardi, Nicola Baldini, Sofia Avnetvisualization. The primary antibodies and concentrations utilized were as follows: anti‐histone H3 1:50 (4499S, Cell Signaling Technology, Danvers, Massachusetts, USA); anti‐acetylated histone H3 1:50 (06–599, Millipore,

(7) QW-5-70 targets the colchicine site and demonstrates antitumor activity in P-gp-overexpressing cancer modelsMol Cancer TherApril 22, 2026Yang Xie, Ruida Hou, Najah Albadari, Hao Chen, Darcie J. Miller, Judith Quadrozzi Quadrozzi Gruntz, Michael L. Oldham, Mir Shahriar Kamal, Jianxiong Jiang, Zhongzhi Wu, et al.(CST, #9114, RRID: AB_2074652), phospho-Histone H3 (CST, #9701, RRID: AB_331535), HistoneH3 (CST, #4499, RRID: AB_10544537), phospho-Bcl-2 (CST, #2827, RRID: AB_659950), MDR1/ABCB1 (P-gp) (CST, #13342, RRID:

(8) Quantitative dissection of the metastatic cascade at single colony resolutionbioRxivApril 22, 2026Chris Roberts, Andy Xu, Xiangwei Fang, Adrienne Visani, Chien-Wei Peng, Xulei Qin, Irenaeus C. C. Chan, Madeline Dunterman, David A. Giles, Yaoxuan You, et al.study: GAPDH (Cell Signaling, 5174S, 1:2000), CREBBP (Cell Signaling, 7389S, 1:200), H3 (Cell Signaling, 4499S, 1:1000), CDX2 (Invitrogen, MA5–35215, 1:2000), H3K27Ac (Cell Signaling, 8173S, 1:1000), Goat-anti-Rabbit IgG Antibody, HRP-conjugate

(9) QW-5-70 targets the colchicine site and demonstrates antitumor activity in P-gp–overexpressing cancer modelsMol Cancer TherApril 22, 2026Yang Xie, Ruida Hou, Najah Albadari, Hao Chen, Darcie J. Miller, Judith Quadrozzi Gruntz, Michael L. Oldham, Mir Shahriar Kamal, Jianxiong Jiang, Zhongzhi Wu, et al.(CST, #9114, RRID: AB_2074652), phospho-Histone H3 (CST, #9701, RRID: AB_331535), HistoneH3 (CST, #4499, RRID: AB_10544537), phospho-Bcl-2 (CST, #2827, RRID: AB_659950), MDR1/ABCB1 (P-gp) (CST, #13342, RRID:

(10) A longitudinal, multi-omic atlas reveals the emergence of a spatially organized immunosuppressive ecosystem in resistant melanomaCell Reports MedicineApril 21, 2026Shiyou Wei, Yulan Deng, Jinho Lee, Hongbin Lan, Zhenyu Yang, Marilyne Labrie, Courtney B. Betts, Hao Duan, Benjamin Tate, Joanna Pucilowska, et al.H3 (clone D1H2) + Mouse anti-Nucleolin (clone NM106) cocktail,Cell Signaling Technology|Abcam,Cat# 4499S; RRID:AB_10544537|Cat# AB215396; RRID:AB_3677433 Rabbit anti-PD-L1 (clone E1L3N),Cell Signaling Technology,Cat#

(11) Epstein-Barr virus orchestrates spatial reorganization and immunomodulation in the classic Hodgkin lymphoma tumor microenvironmentCell Reports MedicineApril 21, 2026Yao Yu Yeo, Huaying Qiu, Yunhao Bai, Bokai Zhu, Yuzhou Chang, Fabio Iannelli, Stephanie Pei Tung Yiu, Jason Yeung, Hendrik A. Michel, Yuchen Wang, et al.EDHu-1),Novus Bio,Clone ID: EDHu-1Cat #: NB110-40686 Histone H3 (Clone D1H2),CST,Clone ID: D1H2Cat #: 4499 CD45RO (Clone UCHL1),BioLegend,Clone ID: UCHL1Cat #: 304202 CD153 (CD30L) (Polyclonal),R&D,Clone ID:

(12) Interaction of NDRG1 and MRE11 Modulates DNA Replication and RepairCancers (Basel)April 20, 2026Hanna M. Doh, Nina Kozlova, Zhipeng A. Wang, Hwan Bae, Philip A. Cole, Taru Muranen(#4895, CST), Vinculin (#13901, CST), Myc (#2276, CST), Tubulin (#ab7291, Abcam, Waltham, MA, USA), H3 (#4499, CST), Cyclin A2 (#), pCDK2 (#2561, CST) Appropriate secondary antibodies (peroxidase-conjugated IgG,

(13) Lysine Acetyltransferase 6A Drives M1 Macrophage Polarization Through Metabolic Reprogramming in Sepsis-Induced Acute Lung InjuryBiomoleculesApril 20, 2026Xin Wang, Junlin Chen, Yimei Lai, Yumeng Wang, Kaixia Hu, Mengshi Wu, Niansheng Yang, Yuefang Huang(1:1000, Cell Signaling Technology, Cat# 8173T), Histone H3 (1:2000, Cell Signaling Technology, Cat# 4499T) and β-actin (1:1000, Servicebio, Wuhan, China, Cat# GB15003). Membranes were washed with TBST, incubated

(14) Chromatin-derived histone proteins from Schmidtea mediterranea function as innate antimicrobial effectorsFrontiers in Cellular and Infection MicrobiologyApril 20, 2026Devika Nampoothiri, Riddhi Bhardwaj, Nimish Deshpande, Prasad Abnaveat room temperature and incubated overnight at 4°C with primary antibodies: anti-histone H3 (CST # 4499, 1:2000), anti-histone H4 (CST # 13919, 1:5000), and anti-histone H2A (Abcam # 18255, 1:5000). After

(15) SREBP2 regulates CCDC25 expression and promotes tumor metastasis in Triple-Negative Breast CancerOncogenesisApril 13, 2026Xingxian Lv, Hao Wang, Beibei Liang, Liyun Yang, Zhuozhuo Han, Wei Xie, Xiuhong Lu, Hao Yang, Gang Huang, Jian ZhaoMPO antibody (50 µg/ml, R&D, USA, #AF3667), H3Cit antibody (1:400, Cell Signaling Technology, USA, #4499S) and Ly6G antibody (1:200, Abcam, #ab238132). After washing, cells were incubated with the secondary

(16) Spermidine Attenuates Neuroimmune Dysfunction in Gulf War Illness via Modulation of the Gut- Brain AxisMol NeurobiolApril 10, 2026Ayushi Trivedi, Subhajit Roy, Madhura More, Dipro Bose, Punnag Saha, Ram Kumar, Subham Sarkar, Jonathan Skupsky, Ashok Tuteja, Kimberly Sullivan, et al.(1:800; 12721S), anti-HO-1 (1:1000; 43966S), anti-β actin (1:2500; 4970S), and anti-Histone H3 (1:1500; 4499S), sourced from Cell Signaling Technology [CST] (Danvers, MA, USA). Species-appropriate HRP-conjugated

(17) HDAC Inhibition Induces Transient Phenotypic Inertia in Dormant OCCC Spheroids by Derepression of Cell Cycle GenesCellsApril 10, 2026Sylvia Cheng, Bart Kolendowski, Yudith Ramos-Valdes, Trevor G. Shepherd, Gabriel E. DiMattia,Cell Signaling Technology,9725 H3K4me3,Cell Signaling Technology,9751 Histone H3,Cell Signaling Technology,4499 Ki67,"Abcam (Cambridge, UK)",ab16667 p-Histone H3 (Ser28),Cell Signaling Technology,9701 Antibodies

(18) Nuclear hexokinase 2 couples hyperglycemia to MYC-driven glycolytic and stemness programs in bladder cancerCell Death & DiseaseApril 8, 2026Shuangjie Liu, Xi Liu, Guangxu Liu, Zheyu Wang, Chengyi Li, Meng Yu, Jihang Yao, Haotian Xing, Yuyan Zhudry milk and incubated overnight at 4 °C with primary antibodies against HK1(#2024, CST), histone H3(#4499, CST), MYC(#5605, CST), Flag tag(#8146, CST), HA tag(#3724, CST), or β-actin(#3700, CST). The membranes

(19) Stress adaptation pathways and HA–CD44 signaling maintain the survival of pancreatic cancer cells with centrosome amplificationCell Communication and SignalingApril 7, 2026Selahattin Can Ozcan, Evrim Goksel, Batuhan Mert Kalkan, Enes Cicek, Beste Kanevetci, Ceyda Acilanantibodies: FLAG (Sigma, F1804, 1:1000), GAPDH (Cell Signaling, 2118, 1:2000), Histone H3 (Cell Signaling, 4499, 1:2000), NRF2 (Cell Signaling, 12721, 1:1000), ATF4 (Cell Signaling, 11815, 1:1000), ATF6 (Cell Signaling,

(20) Cullin-3 adaptor SHKBP1 inhibits SQSTM1/p62 oligomerization and Keap1 sequestrationJ Cell BiolApril 6, 2026Lin Luan, Xiaofu Cao, Zijun Xia, Shivanshi Vaid, Manuel D. Leonetti, Jeremy M. BaskinCell Signaling Technology (2128S, RRID:AB_823664); rabbit anti-Histone H3, Cell Signaling Technology (4499S, RRID:AB_10544537); mouse anti-Nrf2 (A-10), Santa Cruz Biotechnology (sc-365949, RRID:AB_ 10917561).

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6/25/2020Very Good Antibody for H3 Hong SunCan be reused multiple times. Definitely worth buying it.

Histone H3 (D1H2) XP ® Rabbit mAb

Histone H3 (D1H2) XP ® Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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Histone H3 (D1H2) XP ® Rabbit mAb from Cell Signaling Technology

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