H-Ras (259) AC Antibody sc-35 AC from Santa Cruz Biotechnology, Inc.

Product Specs
ItemH-Ras (259) AC Antibody
CompanySanta Cruz Biotechnology, Inc.
Price $416.00Supplier Page View Company Product Page
Catalog Numbersc-35 AC
Antibody TypePrimary Antibody Conjugates
Clonalitymonoclonal
Reactivitymouse, rat, human
FormatPurified
ApplicationsIP
Clone259
IsotypeIgG1
Hostrat
Quantity500 µg/ml, 25% ag
TargetH-Ras
NCBI Full Gene NameNRAS proto-oncogene, GTPase, neuroblastoma ras oncogene, Harvey rat sarcoma virus oncogene, Kirsten rat sarcoma viral oncogene homolog, HRas proto-oncogene, GTPase, KRAS proto-oncogene, GTPase
NCBI Gene Aliasesc-H-ras, Kras2, C-HA-RAS1, RASH1, C-H-RAS, p21ras, HAMSV, ras, ALPS4, HRAS1, 'C-K-RAS, C-BAS/HAS, c-Ki-ras, Ki-ras, NCMS, Ha-ras, c-K-ras, K-Ras 2, K-Ras, K-ras, Hras-1, p21B, H-ras, KI-RAS, K-RAS4A, RASK2, N-ras, K-RAS4B, NRAS1, K-RAS2B, K-RAS2A, C-K-RAS, CTLO, NS3, Harvey-ras, NS6, KRAS, Kras-2, NS, KRAS1, KRAS2, CMNS, OES, c-Ki-ras2, c-rasHa, c-Ha-ras, H-RASIDX, RALD, CFC2, Hras1
Conjugate/Tagagarose

Questions and Answers

Can this antibody be used for studying KRAS mutations in cancer research?

Yes, the H-Ras (259) AC Antibody has been utilized in studies investigating KRAS mutations, particularly in the context of oncogenic properties. For instance, it was employed to immunoprecipitate K-Ras proteins in a study that identified KRAS insertion mutations and their effects on GTPase activity and pathway dependencies in cancer cells ().

What species can I use this antibody with in my experiments?

The H-Ras (259) AC Antibody is reactive with multiple species, including human, mouse, and rat. This broad reactivity allows for its application in various experimental models across different research contexts.

How effective is this antibody for immunoprecipitation assays?

The H-Ras (259) AC Antibody has been shown to effectively immunoprecipitate K-Ras proteins, as demonstrated in a study where it was used to isolate mutant K-Ras proteins for further analysis of their GTPase activity and interactions with other proteins ().

Are there any specific applications or experimental setups where this antibody has been successfully utilized?

This antibody has been successfully used in immunoprecipitation assays to study the functional properties of KRAS mutations, including their impact on GTP hydrolysis rates and interactions with GTPase-activating proteins, as highlighted in recent research ().

Santa Cruz Biotechnology, Inc.

10410 Finnell Street

Dallas, Texas 75220

United States

Phone: 1-800-457-3801
831-457-3801

Company Profile

Website: http://www.scbt.com/

(1) Feedback activation of EGFR/wild-type RAS signaling axis limits KRAS(G12D) inhibitor efficacy in KRAS(G12D)-mutated colorectal cancerOncogeneMay 1, 2023Feng J, Hu Z, Xia X, Liu X, Lian Z, Wang H, Wang L, Wang C, Zhang X, Pang X.

(2) Targeting GRP78 suppresses oncogenic KRAS protein expression and reduces viability of cancer cells bearing various KRAS mutationsNeoplasia (New York, N.Y.)November 1, 2022Ha DP, Huang B, Wang H, Rangel DF, Van Krieken R, Liu Z, Samanta S, Neamati N, Lee AS.

(3) TTYH3 Modulates Bladder Cancer Proliferation and Metastasis via FGFR1/H-Ras/A-Raf/MEK/ERK PathwayInternational journal of molecular sciencesSeptember 10, 2022Biswas PK, Kwak Y, Kim A, Seok J, Kwak HJ, Lee M, Dayem AA, Song K, Park JY, Park KS, et al.

(4) USP28 enables oncogenic transformation of respiratory cells, and its inhibition potentiates molecular therapy targeting mutant EGFR, BRAF and PI3KMolecular oncologySeptember 1, 2022Prieto-Garcia C, Hartmann O, Reissland M, Braun F, Bozkurt S, Pahor N, Fuss C, Schirbel A, Schülein-Völk C, Buchberger A, et al.

(5) Vitexin isolated from Prosopis cineraria leaves induce apoptosis in K-562 leukemia cells via inhibition of the BCR-ABL-Ras-Raf pathwayThe Journal of pharmacy and pharmacologyJanuary 5, 2022Sarkar MK, Kar A, Jayaraman A, Kar Mahapatra S, Vadivel V.

(6) CDK5RAP2 loss-of-function causes premature cell senescence via the GSK3β/β-catenin-WIP1 pathwayCell death & diseaseDecember 20, 2021Wang X, Sipila P, Si Z, Rosales JL, Gao X, Lee KY.

(7) miR-1307 promotes hepatocarcinogenesis by CALR-OSTC-endoplasmic reticulum protein folding pathwayiScienceNovember 19, 2021Xie S, Jiang X, Qin R, Song S, Lu Y, Wang L, Chen Y, Lu D.

(8) Oncogene-dependent sloppiness in mRNA translationMolecular cellNovember 18, 2021Champagne J, Pataskar A, Blommaert N, Nagel R, Wernaart D, Ramalho S, Kenski J, Bleijerveld OB, Zaal EA, Berkers CR, et al.

(9) Apoptotic mechanisms of myricitrin isolated from Madhuca longifolia leaves in HL-60 leukemia cellsMolecular biology reportsJune 1, 2021Sarkar MK, Kar A, Jayaraman A, Shanmugam K, Vadivel V, Mahapatra SK.

(10) Combination of curcumin and ginkgolide B inhibits cystogenesis by regulating multiple signaling pathwaysMolecular medicine reportsMarch 1, 2021Li Y, Gao J, Yang X, Li T, Yang B, Aili A.

(11) Change of Ras and its guanosine triphosphatases (GTPases) during development and regression in bovine corpus luteumTheriogenologyMarch 1, 2020Lee SH, Lee S.

(12) Resistance Mechanisms to SYK Inhibition in Acute Myeloid LeukemiaCancer discoveryFebruary 1, 2020Cremer A, Ellegast JM, Alexe G, Frank ES, Ross L, Chu SH, Pikman Y, Robichaud A, Goodale A, Häupl B, et al.

(13) Novel compound cedrelone inhibits hepatocellular carcinoma progression via PBLD and Ras/Rap1Experimental and therapeutic medicineDecember 1, 2019Wu J, Niu Q, Yuan J, Xu X, Cao L.

(14) The Inhibitory NKR-P1B:Clr-b Recognition Axis Facilitates Detection of Oncogenic Transformation and Cancer ImmunosurveillanceCancer researchJuly 1, 2018Tanaka M, Fine JH, Kirkham CL, Aguilar OA, Belcheva A, Martin A, Ketela T, Moffat J, Allan DSJ, Carlyle JR.

(15) Ganoderma triterpenes retard renal cyst development by downregulating Ras/MAPK signaling and promoting cell differentiationKidney internationalDecember 1, 2017Su L, Liu L, Jia Y, Lei L, Liu J, Zhu S, Zhou H, Chen R, Lu HAJ, Yang B.

(16) KRT19 directly interacts with β-catenin/RAC1 complex to regulate NUMB-dependent NOTCH signaling pathway and breast cancer propertiesOncogeneJanuary 19, 2017Saha SK, Choi HY, Kim BW, Dayem AA, Yang GM, Kim KS, Yin YF, Cho SG.

(17) In vitro and in vivo inhibition of breast cancer cell growth by targeting the Hedgehog/GLI pathway with SMO (GDC-0449) or GLI (GANT-61) inhibitorsOncotargetFebruary 23, 2016Benvenuto M, Masuelli L, De Smaele E, Fantini M, Mattera R, Cucchi D, Bonanno E, Di Stefano E, Frajese GV, Orlandi A, et al.

(18) KRAS insertion mutations are oncogenic and exhibit distinct functional propertiesNature communicationsFebruary 8, 2016White Y, Bagchi A, Van Ziffle J, Inguva A, Bollag G, Zhang C, Carias H, Dickens D, Loh M, Shannon K, et al.were immunoprecipitated by the addition of 20 μl of anti-Ras–agarose slurry (Santa Cruz Biotechnology, sc-35 AC) followed by rocking agitation for 20 min at room temperature. Beads were washed four times with wash

(19) H-ras distribution and signaling in plasma membrane microdomains are regulated by acylation and deacylation eventsMolecular and cellular biologyJune 1, 2015Agudo-Ibáñez L, Herrero A, Barbacid M, Crespo P.

(20) Organizational metrics of interchromatin speckle factor domains: integrative classifier for stem cell adhesion & lineage signalingIntegrative biology : quantitative biosciences from nano to macroApril 1, 2015Vega SL, Dhaliwal A, Arvind V, Patel PJ, Beijer NR, de Boer J, Murthy NS, Kohn J, Moghe PV.

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H-Ras (259) AC Antibody

H-Ras (259) AC Antibody from Santa Cruz Biotechnology, Inc.

Questions and Answers

Santa Cruz Biotechnology, Inc.

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H-Ras (259) AC Antibody from Santa Cruz Biotechnology, Inc.

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