EpCAM (CD326) Chimeric Antibody MA5-48318 from Thermo Fisher Scientific

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Immunocytochemistry/Immunofluorescence analysis of EpCAM (CD326) in Caco-2 cells using EpCAM (CD326) Chimeric Monoclonal Antibody (Product # MA5-48318). Cells were fixed with paraformaldehyde and stained with EpCAM (CD326) Chimeric Monoclonal Antibody at a dilution of 1:100 for 1h followed by Alexa Fluor 488 secondary antibody (1:1,000 dilution), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom EpCAM (CD326) Chimeric Monoclonal Antibody, DAPI, merged channels and an isotype control. The isotype control was a Rabbit IgG Chimeric Monoclonal Antibody (Product # MA5-47825) followed by staining with Alexa Fluor 488 secondary antibody.
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Western Blot analysis of EpCAM (CD326) was performed using EpCAM (CD326) Chimeric Monoclonal Antibody (Product # MA5-48318). A431(A), Caco-2(B), MCF7(C) and HepG2(D) cell lysates (35 µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots probed with EpCAM (CD326) Chimeric Monoclonal Antibody at 2 µg/mL before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

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Thermo Fisher Scientific for
EpCAM (CD326) Chimeric Antibody

Thermo Fisher Scientific's EpCAM (CD326) Chimeric Antibody is a Rabbit monoclonal antibody. The EpCAM (CD326) Chimeric Antibody was generated using EPCAM, and epithelial cell adhesion molecule as the antigen. It reacts with Homo Sapiens, and Human. This antibody has been shown to work in applications such as: EIA, Immunoassay, Staining, ELISA, Flow Cytometry, Immunocytochemistry, and Western Blot.

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EpCAM (CD326) Chimeric Antibody from Thermo Fisher Scientific

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EpCAM (CD326) Chimeric Antibody from Thermo Fisher Scientific

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