Rabbit anti-RNA Polymerase II Antibody, Affinity Purified A300-653A from Bethyl Laboratories, Inc.

Product Specs
ItemRabbit anti-RNA Polymerase II Antibody, Affinity Purified
CompanyBethyl Laboratories, Inc.
Price $433.00Supplier Page View Company Product Page
Catalog NumberA300-653A
Quantity20 µg
ApplicationsWB, IP
Concentration200 µg/ml
Conjugate/TagUnconjugated
FormatWhole IgG
ReactivityHuman, Mouse
TargetRNA Polymerase II
HostRabbit
Antibody TypePrimary
NCBI Full Gene NameRNA polymerase II subunit A
NCBI Gene AliasesRPO2, RPB1, RPOL2, POLRA, NEDHIB, RPBh1, hRPB220, POLR2, RpIILS, hsRPB1
ClonalityPolyclonal
IsotypeWhole IgG
Domain/Region/TerminusBetween 1600 and 1650
Storage2 - 8°C

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Bethyl Laboratories, Inc.

P.O. Box 850

Montgomery, Texas 77356

United States

Phone: 1-800-338-9579
Fax: 1-866-597-6105

Email: orders@fortislife.com

Company Profile

Website: fortislife.com

(1) U2AF2 controls alternative splicing in speckle-proximal regions in an RS domain-dependent mannerNucleic Acids ResearchFebruary 28, 2026Serhii Pankivskyi, Asaki Kobayashi, Jean de Matha Salone, Kimberley Gargoly, David Pastré, Alexandre MaucuerA300-997A; anti-U2AF35 rabbit polyclonal Ab, A302-079A; anti-RNA Polymerase II rabbit polyclonal Ab, A300-653A; anti-Phospho RNA Polymerase II (S2) rabbit polyclonal Ab, A300-654A. Sigma: anti-U2AF2 mouse monoclonal

(2) Mitotic bookmarking by SWI/SNF subunitsNatureJune 1, 2023Zhu Z, Chen X, Guo A, Manzano T, Walsh PJ, Wills KM, Halliburton R, Radko-Juettner S, Carter RD, Partridge JF, et al.primary antibody (H3 (CST, 9715, 1: 10,000); S10P H3 (Millipore, 06–570, 1:1,000); RNA POLII (Bethyl, A300–653A, 1: 500); SOX2 (R&D Systems, AF2018, 1: 200); EZH2 (CST, 5246, 1: 1000); SUZ12 (CST, 3737, 1: 1000);

(3) Active mRNA degradation by EXD2 nuclease elicits recovery of transcription after genotoxic stressNature CommunicationsJanuary 20, 2023Jérémy Sandoz, Max Cigrang, Amélie Zachayus, Philippe Catez, Lise-Marie Donnio, Clèmence Elly, Jadwiga Nieminuszczy, Pietro Berico, Cathy Braun, Sergey Alekseev, et al.Antibodies employed for the PLA assay were as follows: GFP (Roche, 11814460001, 1:500) and RNAPII (Bethyl, A300-653A, 1:1000). Images were acquired with Zeiss Axio Observer Z1 MarianasTM Microscope attached with a CSU-W

(4) DDB1 prepares brown adipocytes for cold-induced thermogenesisLife MetabolismNovember 3, 2022Xu Wang, Shen-Ying Liu, Guo-Sheng Hu, Hao-Yan Wang, Guo-Liang Zhang, Xiang Cen, Si-Ting Xiang, Wen Liu, Peng Li, Haobin Ye, et al.(1:1000, CST, 81464), anti-CDK9 (1:1000, Santa Cruz, sc-13130), anti-RNA Polymerase II (1:1000, Bethyl, A300-653A), anti-GAPDH (1:5000, Proteintech, 60004-1), and anti-Flag M2 (1:1,000, Sigma, F1804). Membranes were

(5) Transcription suppression is mediated by the HDAC1-Sin3 complex in Xenopus nucleoplasmic extractThe Journal of biological chemistryNovember 1, 2022Quaas CE, Lin B, Long DT.07-347), H3K9/14ac (ThermoFisher 49-1010), H3K23ac (Cell Signaling 8848), and RNAPII (Bethyl Laboratories A300-653A). The Xenopus Sin3a antibody was a gift from Dr Peter L. Jones (University of Nevada, Reno). Antibodies

(6) CDK12 orchestrates super-enhancer-associated CCDC137 transcription to direct hepatic metastasis in colorectal cancerClinical and translational medicineOctober 1, 2022Dai W, Wu J, Peng X, Hou W, Huang H, Cheng Q, Liu Z, Luyten W, Schoofs L, Zhou J, et al.11973), CDK13 (BOSTER, A05292‐1), RNA Pol II CTD Ser2 (Bethyl, A300‐654A), total RNA Pol II (Bethyl, A300‐653A), RNA Pol II CTD Ser5 (Bethyl, A304‐408A), Poly ADP‐Ribose Polymerase (PARP) (CST, 9532), Caspase‐3

(7) ZWC complex-mediated SPT5 phosphorylation suppresses divergent antisense RNA transcription at active gene promotersNucleic acids researchApril 22, 2022Park K, Zhong J, Jang JS, Kim J, Kim HJ, Lee JH, Kim J.(48), generated in house; anti-CK2α (ab70774, Abcam); anti-SPT5 (sc-133217, Santa Cruz); anti-RNAPII (A300-653A, Bethyl Laboratories); anti-S5p RNAPII (ab5131, Abcam); anti-Sp2 RNAPII (A300-654A, Bethyl Laboratories);

(8) The Hox transcription factor Ultrabithorax binds RNA and regulates co-transcriptional splicing through an interplay with RNA polymerase IINucleic acids researchJanuary 25, 2022Carnesecchi J, Boumpas P, van Nierop Y Sanchez P, Domsch K, Pinto HD, Borges Pinto P, Lohmann I.1/3000e), Tubulin (Serotec, MCA77G, 1/2000e), GST (Cell signalling, 2624, 1/5000e), Pol II total (Bethyl, A300-653A, 1/2000e), Pol II S5Phos (Bethyl, A304-408A, 1/1000e), Pol II S2Phos (Bethyl, A300-654A, 1/1000e). Protein purification

(9) Transcriptional inhibition by CDK7/9 inhibitor SNS-032 suppresses tumor growth and metastasis in esophageal squamous cell carcinomaCell Death & DiseaseNovember 5, 2021Huishan Zeng, Huiru Yang, Yifan Song, Dong Fang, Liang Chen, Zhijun Zhao, Chaojie Wang, Songqiang Xie(#S1166) were purchased from Selleck Chemicals (Shanghai, China). The antibodies against RNA Pol II (#A300-653A), p-RNA Pol II (S5) (#A304-408A), and p-RNA Pol II (S2) (#A300-654A) were obtained from Bethyl Laboratories

(10) The Protexin complex counters resection on stalled forks to promote homologous recombination and crosslink repairMolecular cellNovember 4, 2021Adeyemi RO, Willis NA, Elia AEH, Clairmont C, Li S, Wu X, D'Andrea AD, Scully R, Elledge SJ.manufacturer’s instructions. Additional antibodies used for PLA include rabbit anti-RNA polymerase II (Bethyl, A300–653A), mouse monoclonal anti- Pol II (8WG16) (Santa Cruz, sc-56767) and anti-DNA-RNA Hybrid Antibody, clone S9.6

(11) Transcriptional control of DNA repair networks by CDK7 regulates sensitivity to radiation in MYC-driven medulloblastomaCell reportsApril 27, 2021Veo B, Danis E, Pierce A, Wang D, Fosmire S, Sullivan KD, Joshi M, Khanal S, Dahl N, Karam S, et al.#2916, α-CDK9 #2316, α-PARP #9542, α-cMYC #5605 (Cell Signaling Technology), and α-Total RNA Pol II #A300–653A (Bethyl Laboratories) were exposed overnight at 4°C. Secondary antibody, α-mouse-HRP Cat# 7076 and α-rabbit-HRP

(12) A hypermethylation strategy utilized by enhancer-bound CARM1 to promote estrogen receptor α-dependent transcriptional activation and breast carcinogenesisTheranosticsJanuary 1, 2020Peng BL, Li WJ, Ding JC, He YH, Ran T, Xie BL, Wang ZR, Shen HF, Xiao RQ, Gao WW, et al.temperature (RT) (for MED12 (Bethyl Laboratory Inc., A300-774A) and Pol II (Bethyl Laboratory Inc. A300-653A) ChIP), or fixed with disuccinimidyl glutarate (DSG) (2 mM) (Proteochem) for 45 mins at RT, washed twice

(13) Involvement of condensin in cellular senescence through gene regulation and compartmental reorganizationNature communicationsDecember 12, 2019Iwasaki O, Tanizawa H, Kim KD, Kossenkov A, Nacarelli T, Tashiro S, Majumdar S, Showe LC, Zhang R, Noma KI.mouse monoclonal anti-FLAG M2 (Sigma–Aldrich), rabbit polyclonal anti-Pol II (Bethyl Laboratories, A300-653A), rabbit polyclonal anti-Myc (Abcam, ab9106), and mouse monoclonal anti-Pol II (Covance, 8WG16). Cells

(14) de novo MEPCE nonsense variant associated with a neurodevelopmental disorder causes disintegration of 7SK snRNP and enhanced RNA polymerase II activationScientific reportsAugust 29, 2019Schneeberger PE, Bierhals T, Neu A, Hempel M, Kutsche K.Proteintech, #14917-1-AP); rabbit polyclonal anti-RNA Polymerase II (1:1,000, Bethyl Laboratories, #A300-653A); rabbit polyclonal anti-phospho RNA polymerase II (S2) (1:1,000, Bethyl Laboratories, #A300-654A);

(15) Lineage-specific control of TFIIH by MITF determines transcriptional homeostasis and DNA repairOncogeneMay 1, 2019Seoane M, Buhs S, Iglesias P, Strauss J, Puller AC, Müller J, Gerull H, Feldhaus S, Alawi M, Brandner JM, et al.cyclin H (Abcam, ab92376), anti-human CDK2 (Santa Cruz, sc-53220), anti-human RNA polymerase II (Bethyl, A300-653A), anti-human phospho-RNA polymerase II (S2) (Bethyl, A300-654A), anti-human phospho-RNA polymerase II

(16) Small-molecule targeting of brachyury transcription factor addiction in chordomaNature medicineFebruary 1, 2019Sharifnia T, Wawer MJ, Chen T, Huang QY, Weir BA, Sizemore A, Lawlor MA, Goodale A, Cowley GS, Vazquez F, et al.(clone 4E12, #04–1570-I; 1:1,000) were purchased from Millipore. The antibody against total POLR2A (#A300–653A; 1:400) was purchased from Bethyl Laboratories. The antibody against the V5 tag (#46–0705, 1:5,000)

(17) Targeting MYC dependency in ovarian cancer through inhibition of CDK7 and CDK12/13eLifeNovember 13, 2018Zeng M, Kwiatkowski NP, Zhang T, Nabet B, Xu M, Liang Y, Quan C, Wang J, Hao M, Palakurthi S, et al.(ABCAM # ab32072), anti-MCL-1(S-19) (Santa Cruz # sc-819), anti-RNA polymerase II (Bethyl Laboratories #A300-653A), anti-RNA polymerase II subunit B1 (phospho CTD Ser-2, clone 3E10) (Millipore #04–1571), anti-RNA polymerase II subunit B1

(18) Multiple CDK inhibitor dinaciclib suppresses neuroblastoma growth via inhibiting CDK2 and CDK9 activityScientific reportsJuly 5, 2016Chen Z, Wang Z, Pang JC, Yu Y, Bieerkehazhi S, Lu J, Hu T, Zhao Y, Xu X, Zhang H, et al.(Sigma-Aldrich Corp, St. Louis, MO, USA). Anti-phospho-RNAP II (Ser2) (A300-654A) and anti-RNAP II (A300-653A) antibodies were from Bethyl Laboratories (Bethyl Laboratories, Inc., Montgomery, TX, USA). Anti-CDK2 (M2

(19) A selective chemical probe for exploring the role of CDK8 and CDK19 in human diseaseNature chemical biologyDecember 1, 2015Dale T, Clarke PA, Esdar C, Waalboer D, Adeniji-Popoola O, Ortiz-Ruiz MJ, Mallinger A, Samant RS, Czodrowski P, Musil D, et al.Phospho-POLR2ASER2 (#A300-654A), Phospho-POLR2ASER5 (Bethyl Labs, #A300-655A) or total POLR2A (Bethyl Labs, #A300-653A). After washing with TBS-T, blots were incubated with HRP-conjugated secondary antibodies in blocking

(20) CDK9 inhibition strategy defines distinct sets of target genesBMC Research NotesDecember 1, 2014Judit Garriga, Xavier GrañaWestern blot analyses were performed as described previously [6] using the following antibodies: Anti-RNAPII (A300-653A), anti-Ser-2 (A300-654A), and anti-Ser-5 (A300-655A) rabbit polyclonal antibodies were obtained from

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Rabbit anti-RNA Polymerase II Antibody, Affinity Purified from Bethyl Laboratories, Inc.

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Bethyl Laboratories, Inc.

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Rabbit anti-RNA Polymerase II Antibody, Affinity Purified from Bethyl Laboratories, Inc.

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