Western Blot analysis of PIM2 in HEK293 lysate (10 µg protein in RIPA buffer) using PIM2 Polyclonal Antibody (Product # PA5-142720) at 0.1 µg/mL. Lane A: HEK293 lysate over expressing Human PIM2 with C-terminal MYC tag probed with PIM2 Antibody. Lane B: Mock-transfected HEK293 probed with PIM2 Antibody. Lane C: HEK293 lysate over expressing Human PIM2 with DYKDDDDK tag probed with anti-MYC Tag (1:1,000). Detected by chemiluminescence.