Western Blot of SETX in whole cell lysate (50 µg) from HeLa, HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28, Jurkat, K-562, RMPI-8226, and 786-O cells. Samples were treated with NETN lysis buffer, incubated with SETX monoclonal antibody (Product # MA5-44294) at a dilution of 1:1000, followed by HRP-conjugated goat anti-rabbit IgG (exposure time of 30 seconds).