Western blot analysis of SARS-CoV-2 Spike Protein RBD in HEK293 cell lysates. Samples were incubated in SARS-CoV-2 Spike Protein RBD monoclonal antibody (Product # MA5-47449) using a dilution of 1:3,000 (green). [1] protein standard, [2] non-transfected cells, [3] cells transfected with pCI-Neo-Mod containing the SARS-CoV2-bd cDNA, and [4] cells transfected with pCI-Neo-GFP vector expression construct containing containing the SARS-CoV2-bd cDNA. The band at 25 kDa mark in the transfected cells demonstrates expression of SARS-CoV-bd protein, and the band at about 50 kDa corresponds to a GFP-SARS-CoV2-bd fusion protein. The same blot was simultaneously probed with rabbit pAb to HSP60 (Product # PA5-143609) dilution 1:5,000, in red, revealing a single band at 60 kDa in both transfected and non-transfected cells.