Western blot analysis of USO1 in different lysates (10 µg/Lane). Exposure time: 1 minute; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. Samples were incubated in USO1 Monoclonal antibody (Product # MA5-44748) using a dilution of 1:1,000 in 5% NFDM/TBST at room temperature for 2 hours followed by Goat Anti-Rabbit IgG - HRP secondary antibody at a dilution of 1:300,000 for 1 hour at room temperature. Lane 1: Hela cell lysate; Lane 2: 293T cell lysate; Lane 3: HepG2 cell lysate; Lane 4: A431 cell lysate. Predicted band size: 108 kDa. Observed band size: 108 kDa.