Western blot analysis of Fibrillarin in different tissue and cell line lysates. Samples were incubated in Fibrillarin monoclonal antibody (Product # MA5-47424) using a dilution of 1:2,000 (green). [1] protein standard (red), [2] rat whole brain, [3] mouse whole brain, [4] NIH-3T3 cells,[5] HEK293, [6] HeLa, and [7] SH-SY5Y cells. Strong band at ~35 kDa corresponds to fibrillarin protein seen in all cell line lysates. A much weaker band is seen in tissue lysates since fibrillarin is more heavily expressed in rapidly dividing cells. The blot simultaneously was probed with rabbit pAb to UCHL1, dilution 1:3,000, in red, revealing the ~25 kDa UCHL1 protein in lysates of tissues containing neurons or cells with neuronal properties.