Immunohistochemical staining of human tonsil tissue using anti-CD3E antibody (TA385773). YTH 12.5 Anti-CD3E staining of paraffin embedded human tissue using the rabbit-chimeric version of YTH 12.5 (TA385773). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with TA385773 was carried out at 4 µg/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Strong membrane staining of T-cells at the periphery of the germinal centre and between follicles may be observed. Recommended concentration, 2-4 µg/ml.

Flow-cytometry using anti-CD3 antibody YTH 12.5 (TA385773). Rhesus monkey lymphocytes were stained with an isotype control (TA385644, red) or the rabbit-chimeric version of YTH 12.5 (TA385773, blue) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FlowJo single-cell flow cytometer.

Flow-cytometry using the anti-CD3E antibody YTH 12.5 (TA385773). Jurkat cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of YTH 12.5 (TA385773, blue line) at a concentration of 10 µg/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 µg/ml and cells analyzed on a FACSCanto flow-cytometer.

Flow-cytometry using anti-CD3 epsilon antibody YTH 12.5 (TA385773). Human lymphocytes were stained with an isotype control (TA385644, panel A) or the rabbit-chimeric version of YTH 12.5 (TA385773, panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.