Western blot analysis of extracts of various cell lines, using Phospho-CRMP2/DPYSL2-T514 antibody (TA375609) at 1:1000 dilution or CRMP2/DPYSL2 antibody (A14570). SH-SY5Y cells were treated by CIP(20uL/400ul) at 37‚ÑÉ for 1 hour. PC-12 cells were treated by CIP(20uL/400ul) at 37‚ÑÉ for 1 hour. |Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit .|Exposure time: 1s.