Western Blot using anti-Alpha-fetoprotein antibody AF5 (TA385816) HepG2 cell lysate (35µg protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of AF5 (TA385816) at 1 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Alpha-fetoprotein is 68.7kDa, though this protein is also glycosylated (Uniprot ID: P02771). TA385816 successfully detected human Alpha-fetoprotein in HepG2 cell lysates.