Western blot analysis of extract Western Blot analysis of extracts from MCF-7 cells, untreated or treated with doxorubicin (0.5uM, 36h), doxorubicin followed by Lambda Phosphatase (10,000Units/ml, 1h), nocodazole (50ng/ml, 36h) or nocodazole followed by Lambda Phosphatase, using R2039-22M (upper) or p53 Antibody (lower).s from MCF-7 cells, untreated or treated with doxorubicin (0.5 uM, 36h), doxorubicin followed by Lambda Phosphatase (10,000 Units/ml, 1h), nocodazole (50ng/ml, 36h) or nocodazole followed by Lambda Phosphatase, using R2039-22M (upper) or p53 Antibody (lower).